PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0072 [new locus tag: SAUSA300_RS00365 ]
• pan locus tag^?: SAUPAN000820000
• symbol: SAUSA300_0072
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0072 [new locus tag: SAUSA300_RS00365 ]
• symbol: SAUSA300_0072
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 79759..80397
• length: 639
• essential: unknown

⊟Accession numbers

• Gene ID: 3914144 NCBI
• RefSeq: YP_492790 NCBI
• BioCyc: GH3C-71 BioCyc
• MicrobesOnline: 1291587 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGCACGAGACCATCAAGAAGCTTGGGAGAGAACAATAAAAATGCTTGATACATCTGAC
  ATCGAAGGGAAAACGATTTTAGATGTGGGATGTAATCAAGGCGGATTTTTACGACAGTTA
  TACGATACAACACCGTTTAAAAAAGGTGTTGGCATAGATTTAGCACGTTTATCTTTGGAA
  AAGGCAGAGACATTAAAAGGACAACGTCCACTTACATACTATTTAACAGATAAACCGCAA
  GAAACGAAGCACGTGTTTGATACGGCAGTAAGTACGTCTGTCTTGTACTTAATAGAAGAT
  ATTCCGCAACATGCAAAAGATTTAAAAGAGGTATTGAAACCAGGCGGTGTTTATTACGCT
  TCATTCGCGGATTTAACTAATAACCCAAGTCGTCAGTTTATGGATGACACGATTAATCAA
  TATGGTGCAACACCTTCTCAGAATCACTCTCTAAAACATATCGTTGATAGCTTTGTGGAT
  GCAGGATTTGAAGTTGCAGTAATGAAAGAGCATGTACCTGACGTGATTGATTTAACACAT
  TATAGCGATTTTTATTTATCACCGAATGATTATTTACAAACACTATATGAAGAATCGTTT
  TTAATAAAAGCAAGTGTGAAAGAAGGTACTGAGAAATGA
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⊟Protein

⊟General

• locus tag: SAUSA300_0072 [new locus tag: SAUSA300_RS00365 ]
• symbol: SAUSA300_0072
• description: hypothetical protein
• length: 212
• theoretical pI: 4.84187
• theoretical MW: 24017.8
• GRAVY: -0.46934

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 35.3)
  Unknown function Enzymes of unknown specificity tRNA (mo5U34)-methyltransferase (TIGR00452; EC 2.1.1.-; HMM-score: 31)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 30.8)
  and 10 more
  2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 19.9)
  Genetic information processing Transcription RNA processing 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 19.1)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 19.1)
  pseudaminic acid biosynthesis-associated methylase (TIGR03587; HMM-score: 18.8)
  methyltransferase, Rta_06860 family (TIGR04290; EC 2.1.1.-; HMM-score: 18.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs putative 4-mercaptohistidine N1-methyltranferase (TIGR04345; HMM-score: 17.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 17.4)
  Metabolism Amino acid biosynthesis Aspartate family methionine biosynthesis protein MetW (TIGR02081; HMM-score: 14.3)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 12.4)
  Unknown function General TlyA family rRNA methyltransferase/putative hemolysin (TIGR00478; HMM-score: 11.5)
• TheSEED  :
  • FIG01108754: hypothetical protein
• PFAM:
  NADP_Rossmann (CL0063) Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 49.3)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 41.5)
  and 11 more
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 39.4)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 37.9)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 33.6)
  Methyltransf_9; Protein of unknown function (DUF1698) (PF08003; HMM-score: 28.1)
  NodS; Nodulation protein S (NodS) (PF05401; HMM-score: 20.7)
  FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 13.5)
  Uteroglobin (CL0370) Feld-I_B; Allergen Fel d I-B chain (PF09252; HMM-score: 13)
  NADP_Rossmann (CL0063) Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 12.4)
  Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 12)
  CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 11.5)
  MetW; Methionine biosynthesis protein MetW (PF07021; HMM-score: 11.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006116
  • TAT(Tat/SPI): 0.000398
  • LIPO(Sec/SPII): 0.005652
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160872 NCBI
• RefSeq: YP_492790 NCBI
• UniProt: A0A0H2XG45 UniProt

⊟Protein sequence

• MARDHQEAWERTIKMLDTSDIEGKTILDVGCNQGGFLRQLYDTTPFKKGVGIDLARLSLEKAETLKGQRPLTYYLTDKPQETKHVFDTAVSTSVLYLIEDIPQHAKDLKEVLKPGGVYYASFADLTNNPSRQFMDDTINQYGATPSQNHSLKHIVDSFVDAGFEVAVMKEHVPDVIDLTHYSDFYLSPNDYLQTLYEESFLIKASVKEGTEK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0072 > SAUSA300_0073 > opp-3B > opp-3C > SAUSA300_0076 > SAUSA300_0077

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications