PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0084 [new locus tag: SAUSA300_RS00435 ]
• pan locus tag^?: SAUPAN000488000
• symbol: SAUSA300_0084
• pan gene symbol^?: cstR
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0084 [new locus tag: SAUSA300_RS00435 ]
• symbol: SAUSA300_0084
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 92713..92973
• length: 261
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913845 NCBI
• RefSeq: YP_492802 NCBI
• BioCyc: see SAUSA300_RS00435
• MicrobesOnline: 1291599 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  ATGAATTATGATAAAAAAATGATTAATCGTATTAATAGAATACAAGGGCAACTAAATGGA
  ATTATTAAAATGATGGAGGAAGGAAAAGACTGTAAAGATGTCATTACACAAATAAGTGCA
  TCAAAGAGTTCACTCCAACGCTTGATGGGTATCATTATTAGTGAGAATTTAATAGAATGT
  GTAAAAGCAGCTGCGGATGATGAAGAAAGCTCCCAAGAGTTAATTAATGAAGCTGTAAAC
  TTATTGGTGAAAAGTAAGTAA
  60
  120
  180
  240
  261
  

⊟Protein

⊟General

• locus tag: SAUSA300_0084 [new locus tag: SAUSA300_RS00435 ]
• symbol: SAUSA300_0084
• description: hypothetical protein
• length: 86
• theoretical pI: 5.12449
• theoretical MW: 9641.17
• GRAVY: -0.293023

⊟Function

• TIGRFAM:
• TheSEED  :
  • FIG007303: uncharacterized protein
• PFAM:
  no clan defined Trns_repr_metal; Metal-sensitive transcriptional repressor (PF02583; HMM-score: 87.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004536
  • TAT(Tat/SPI): 0.000986
  • LIPO(Sec/SPII): 0.000445
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159958 NCBI
• RefSeq: YP_492802 NCBI
• UniProt: A0A0H2XFN1 UniProt

⊟Protein sequence

• MNYDKKMINRINRIQGQLNGIIKMMEEGKDCKDVITQISASKSSLQRLMGIIISENLIECVKAAADDEESSQELINEAVNLLVKSK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0083 < SAUSA300_0084

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Justin L Luebke, Jiangchuan Shen, Kevin E Bruce, Thomas E Kehl-Fie, Hui Peng, Eric P Skaar, David P Giedroc
The CsoR-like sulfurtransferase repressor (CstR) is a persulfide sensor in Staphylococcus aureus.
Mol Microbiol: 2014, 94(6);1343-60
[PubMed:25318663] [WorldCat.org] [DOI] (I p)Jiangchuan Shen, Mary E Keithly, Richard N Armstrong, Khadine A Higgins, Katherine A Edmonds, David P Giedroc
Staphylococcus aureus CstB Is a Novel Multidomain Persulfide Dioxygenase-Sulfurtransferase Involved in Hydrogen Sulfide Detoxification.
Biochemistry: 2015, 54(29);4542-54
[PubMed:26177047] [WorldCat.org] [DOI] (I p)