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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0918 [new locus tag: SAUSA300_RS04935 ]
  • pan locus tag?: SAUPAN003195000
  • symbol: SAUSA300_0918
  • pan gene symbol?: ugtP
  • synonym:
  • product: diacylglycerol glucosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0918 [new locus tag: SAUSA300_RS04935 ]
  • symbol: SAUSA300_0918
  • product: diacylglycerol glucosyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1007012..1008187
  • length: 1176
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGTTACTCAAAATAAAAAGATATTGATTATTACTGGCTCATTCGGTAACGGTCATATG
    CAAGTTACACAGAGTATCGTTAATCAACTTAATGATATGAATCTAGACCATTTAAGCGTC
    ATTGAGCACGATTTATTTATGGAAGCTCATCCAATTTTGACTTCTATTTGTAAAAAATGG
    TATATCAATAGCTTTAAATATTTTAGAAATATGTACAAAGGGTTTTATTACAGCCGCCCA
    GATAAACTAGACAAATGTTTTTACAAATACTATGGACTTAATAAGTTAATTAATTTATTG
    ATAAAAGAAAAGCCAGATTTAATATTATTAACGTTTCCTACACCAGTTATGTCGGTACTA
    ACTGAGCAATTTAACATTAATATTCCAGTTGCTACAGTGATGACAGACTATCGCTTACAT
    AAAAACTGGATTACGCCGTATTCAACAAGATATTATGTGGCAACAAAAGAAACGAAACAA
    GACTTCATAGACGTAGGTATTGATCCTTCAACAGTTAAAGTGACAGGTATTCCTATTGAT
    AACAAATTTGAAACGCCTATTAATCAAAAGCAGTGGTTAATAGACAACAACTTAGATCCA
    GATAAGCAAACTATTTTAATGTCAGCTGGTGCATTTGGTGTATCTAAAGGTTTTGACACG
    ATGATTACTGATATATTAGCGAAAAGTGCAAATGCACAAGTAGTTATGATTTGTGGTAAG
    AGCAAAGAGCTAAAGCGTTCTTTAACAGCTAAGTTTAAATCTAACGAGAATGTATTGATT
    CTAGGTTATACCAAACACATGAATGAATGGATGGCATCAAGTCAACTTATGATTACGAAA
    CCTGGTGGTATCACAATAACTGAAGGTTTCGCCCGTTGTATTCCAATGATTTTCCTAAAT
    CCTGCACCTGGTCAAGAGCTTGAAAATGCCCTTTACTTTGAAGAAAAAGGTTTTGGTAAA
    ATCGCTGATACTCCAGAAGAAGCAATAAAAATTGTAGCAAGTTTAACTAACGGTAATGAG
    CAATTAACTAACATGATTAGTACAATGGAACAAGATAAAATTAAGTATGCTACACAGACA
    ATATGTCGAGACTTACTTGATTTAATCGGTCATTCATCACAACCACAAGAGATTTATGGA
    AAGGTTCCTTTATATGCAAGATTCTTCGTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1176

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0918 [new locus tag: SAUSA300_RS04935 ]
  • symbol: SAUSA300_0918
  • description: diacylglycerol glucosyltransferase
  • length: 391
  • theoretical pI: 8.60534
  • theoretical MW: 44547.5
  • GRAVY: -0.180563

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.1.315?  ExPASy
    Diglucosyl diacylglycerol synthase (1,6-linking) UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP UDP-glucose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol?
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (TIGR01133; EC 2.4.1.227; HMM-score: 41.9)
    and 3 more
    UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase (TIGR03590; EC 3.6.1.57; HMM-score: 23.5)
    Hypothetical proteins Conserved conserved hypothetical protein (TIGR00661; HMM-score: 20.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 11.5)
  • TheSEED  :
    • diglucosyldiacylglycerol synthase (LTA membrane anchor synthesis)
    Cell Wall and Capsule Gram-Positive cell wall components Teichoic and lipoteichoic acids biosynthesis  diglucosyldiacylglycerol synthase (LTA membrane anchor synthesis)
  • PFAM:
    GT-B (CL0113) MGDG_synth; Monogalactosyldiacylglycerol (MGDG) synthase (PF06925; HMM-score: 162.4)
    and 4 more
    Glyco_tran_28_C; Glycosyltransferase family 28 C-terminal domain (PF04101; HMM-score: 40.6)
    Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 33)
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 22.7)
    Epimerase_2; UDP-N-acetylglucosamine 2-epimerase (PF02350; HMM-score: 19.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011009
    • TAT(Tat/SPI): 0.000161
    • LIPO(Sec/SPII): 0.002461
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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