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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0918 [new locus tag: SAUSA300_RS04935 ]
- pan locus tag?: SAUPAN003195000
- symbol: SAUSA300_0918
- pan gene symbol?: ugtP
- synonym:
- product: diacylglycerol glucosyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0918 [new locus tag: SAUSA300_RS04935 ]
- symbol: SAUSA300_0918
- product: diacylglycerol glucosyltransferase
- replicon: chromosome
- strand: -
- coordinates: 1007012..1008187
- length: 1176
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3912877 NCBI
- RefSeq: YP_493618 NCBI
- BioCyc: see SAUSA300_RS04935
- MicrobesOnline: 1292433 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGGTTACTCAAAATAAAAAGATATTGATTATTACTGGCTCATTCGGTAACGGTCATATG
CAAGTTACACAGAGTATCGTTAATCAACTTAATGATATGAATCTAGACCATTTAAGCGTC
ATTGAGCACGATTTATTTATGGAAGCTCATCCAATTTTGACTTCTATTTGTAAAAAATGG
TATATCAATAGCTTTAAATATTTTAGAAATATGTACAAAGGGTTTTATTACAGCCGCCCA
GATAAACTAGACAAATGTTTTTACAAATACTATGGACTTAATAAGTTAATTAATTTATTG
ATAAAAGAAAAGCCAGATTTAATATTATTAACGTTTCCTACACCAGTTATGTCGGTACTA
ACTGAGCAATTTAACATTAATATTCCAGTTGCTACAGTGATGACAGACTATCGCTTACAT
AAAAACTGGATTACGCCGTATTCAACAAGATATTATGTGGCAACAAAAGAAACGAAACAA
GACTTCATAGACGTAGGTATTGATCCTTCAACAGTTAAAGTGACAGGTATTCCTATTGAT
AACAAATTTGAAACGCCTATTAATCAAAAGCAGTGGTTAATAGACAACAACTTAGATCCA
GATAAGCAAACTATTTTAATGTCAGCTGGTGCATTTGGTGTATCTAAAGGTTTTGACACG
ATGATTACTGATATATTAGCGAAAAGTGCAAATGCACAAGTAGTTATGATTTGTGGTAAG
AGCAAAGAGCTAAAGCGTTCTTTAACAGCTAAGTTTAAATCTAACGAGAATGTATTGATT
CTAGGTTATACCAAACACATGAATGAATGGATGGCATCAAGTCAACTTATGATTACGAAA
CCTGGTGGTATCACAATAACTGAAGGTTTCGCCCGTTGTATTCCAATGATTTTCCTAAAT
CCTGCACCTGGTCAAGAGCTTGAAAATGCCCTTTACTTTGAAGAAAAAGGTTTTGGTAAA
ATCGCTGATACTCCAGAAGAAGCAATAAAAATTGTAGCAAGTTTAACTAACGGTAATGAG
CAATTAACTAACATGATTAGTACAATGGAACAAGATAAAATTAAGTATGCTACACAGACA
ATATGTCGAGACTTACTTGATTTAATCGGTCATTCATCACAACCACAAGAGATTTATGGA
AAGGTTCCTTTATATGCAAGATTCTTCGTTAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0918 [new locus tag: SAUSA300_RS04935 ]
- symbol: SAUSA300_0918
- description: diacylglycerol glucosyltransferase
- length: 391
- theoretical pI: 8.60534
- theoretical MW: 44547.5
- GRAVY: -0.180563
⊟Function[edit | edit source]
- reaction: EC 2.4.1.315? ExPASyDiglucosyl diacylglycerol synthase (1,6-linking) UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP UDP-glucose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol?
- TIGRFAM: Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (TIGR01133; EC 2.4.1.227; HMM-score: 41.9)and 3 moreUDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase (TIGR03590; EC 3.6.1.57; HMM-score: 23.5)Hypothetical proteins Conserved conserved hypothetical protein (TIGR00661; HMM-score: 20.5)Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 11.5)
- TheSEED :
- diglucosyldiacylglycerol synthase (LTA membrane anchor synthesis)
- PFAM: GT-B (CL0113) MGDG_synth; Monogalactosyldiacylglycerol (MGDG) synthase (PF06925; HMM-score: 162.4)and 4 moreGlyco_tran_28_C; Glycosyltransferase family 28 C-terminal domain (PF04101; HMM-score: 40.6)Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 33)Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 22.7)Epimerase_2; UDP-N-acetylglucosamine 2-epimerase (PF02350; HMM-score: 19.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.011009
- TAT(Tat/SPI): 0.000161
- LIPO(Sec/SPII): 0.002461
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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