PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0917 [new locus tag: SAUSA300_RS04930 ]
• pan locus tag^?: SAUPAN003193000
• symbol: SAUSA300_0917
• pan gene symbol^?: ltaA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0917 [new locus tag: SAUSA300_RS04930 ]
• symbol: SAUSA300_0917
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1005844..1007052
• length: 1209
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912948 NCBI
• RefSeq: YP_493617 NCBI
• BioCyc: see SAUSA300_RS04930
• MicrobesOnline: 1292432 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  
  ATGGAAAGGTTCCTTTATATGCAAGATTCTTCGTTAAATAATTACGCTAATCATAAAAAT
  TTCATTTTAATGCTTATTATCTTATTTTTAATGGAATTTGCGAGAGGCATGTATATCCTA
  AGCTATATTAATTTCTTGCCAACAGTTACATCCATTGCTGTAGCAATAACGTCTCTAGCA
  TTTTCAATACATTTTATTGCTGATGCATCTACAAACTTTGTTATAGGATTTTTACTGAAA
  AAATTCGGCACAAAAATCGTTTTAACGACTGGATTTATTTTAGCATTTACAAGTTTATTT
  TTAGTTATTTGGTTTCCAGCATCACCTTTTGTAATCATATTTAGTGCTATGATGCTCGGT
  ATTGCAGTTAGTCCTATTTGGGTCATTATGTTATCCAGTGTTGAAGAAGATAAACGTGGC
  AAACAAATGGGCTATGTTTATTTTTCATGGTTGTTAGGCCTATTGGTAGGTATGGTTTTT
  ATGAATCTACTTATCAAAGTGCACCCAACACGCTTTGCCTTCATGATGTCTTTAGTCGTT
  TTAATTGCATGGATATTATATTATTTTGTCGATGTAAAGTTAACAAATTATAATACTCGT
  CCAGTTAAAGCTCAATTGAGACAAATTGTTGATGTGACAAAGCGCCATTTATTGTTGTTC
  CCTGGTATTCTTTTACAAGGTGCCGCAATTGCTGCCCTAGTTCCTATATTACCAACATAT
  GCTACTAAGGTTATTAACGTAAGCACAATTGAATATACTGTTGCAATCATTATTGGTGGT
  ATCGGCTGTGCAGTTTCGATGCTATTTTTATCGAAACTAATAGATAACCGCAGCAGAAAC
  TTTATGTATGGTGTTATTCTAAGCGGATTTATTTTATACATGATATTAATTTTTACTCTA
  TCTATGATTGTTAATATTCACATCTTGTGGATTATCGCTTTAGCTATCGGTCTAATGTAT
  GGCATCTTATTACCAGCATGGAATACCTTTATGGCACGATTTATCAAATCAGATGAACAA
  GAAGAAACTTGGGGTGTTTTTAATAGTATCCAAGGATTCGGCTCAATGATAGGGCCATTA
  TTTGGCGGATTAATTACACAATTTACCAACAATTTAAACAATACATTTTATTTCTCGGCA
  TTAATCTTTTTAGTCTTAGCTGTATTTTATGGAAGCTACTTTATAGTAAATAGAGAAAAA
  GCTAAGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1209
  

⊟Protein

⊟General

• locus tag: SAUSA300_0917 [new locus tag: SAUSA300_RS04930 ]
• symbol: SAUSA300_0917
• description: hypothetical protein
• length: 402
• theoretical pI: 9.88498
• theoretical MW: 45457.4
• GRAVY: 0.936318

⊟Function

• TIGRFAM:
  multidrug resistance protein (TIGR00880; HMM-score: 49.9)
  and 5 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 27.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 19.5)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 10)
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 6.7)
  Metabolism Transport and binding proteins Other glycerol-3-phosphate transporter (TIGR00712; HMM-score: 5.2)
• TheSEED  :
  • FIG01107905: hypothetical protein
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 132)
  and 2 more
  MFS_1_like; MFS_1 like family (PF12832; HMM-score: 15.5)
  ATG22; Vacuole effluxer Atg22 like (PF11700; HMM-score: 7.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007922
  • TAT(Tat/SPI): 0.000126
  • LIPO(Sec/SPII): 0.001861
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 87160445 NCBI
• RefSeq: YP_493617 NCBI
• UniProt: Q2FI61 UniProt

⊟Protein sequence

• MERFLYMQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVTSIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPASPFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIKVHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGILLQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFMYGVILSGFILYMILIFTLSMIVNIHILWIIALAIGLMYGILLPAWNTFMARFIKSDEQEETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIVNREKAK

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0917 < SAUSA300_0918

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications