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⊟Summary[edit | edit source]

  • pan ID?: SAUPAN003195000
  • symbol?: ugtP
  • synonym:
  • description?: diacylglycerol glucosyltransferase

      descriptions from strain specific annotations:

    • diacylglycerol glucosyltransferase
    • diacylglycerol beta-glucosyltransferase
    • diglucosyl diacylglycerol synthase
    • UDP-glucose diacylglycerol glucosyltransferase, putative
    • diglucosyldiacylglycerol synthase (LTA membrane anchor synthesis)
    • processive diacylglycerol glucosyltransferase
    • 1,2-diacylglycerol 3-glucosyltransferase
    • monogalactosyldiacylglycerol (MGDG) synthase family protein
    • processive diacylglycerol glucosyltransferase YpfP
    • UDP-glucose diacylglycerol glucosyltransferase
  • strand?: -
  • coordinates?: 3586499..3587674
  • synteny block?: BlockID0022820
  • occurrence?: in 100% of 34 strains

ypfP (ugtP) : diacylglycerol glucosyltransferase [1]

Formation of diglucosyl-diacylglycerol on the inner leaflet of the cell membrane by diacylglycerol glucosyltransferase is the first committed step in teichoic acid synthesis. YpfP catalyzes the iterative, processive transfer of glucose from two equivalents of cytosolic UDP-glucose onto a membrane-bound diacylglycerol anchor, providing the platform upon which lipoteichoic acids will eventually be built. This activity is regulated by the membrane protein-processing metalloprotease FtsH which, when activated, degrades YpfP, halting lipoteichoic acid biosynthesis until new YpfP enzyme can be synthesized. Its activity can also be modulated allosterically by CozEa and CozEb proteins although they have a more dramatic role on the activity of the subsequent enzyme in the pathway, diglucosyl-diacylglycerol flippase.

⊟Orthologs[edit | edit source]

    COL:
    SACOL1022 (ypfP)
    N315:
    SA0875
    NCTC8325:
    SAOUHSC_00953
    Newman:
    NWMN_0887 (murG)
    USA300_FPR3757:
    SAUSA300_0918
    04-02981:
    SA2981_0971
    08BA02176:
    C248_1041
    11819-97:
    MS7_0973 (ugtP)
    6850:
    RSAU_000902 (ugtP)
    71193:
    ST398NM01_1013
    ECT-R 2:
    ECTR2_872
    ED133:
    SAOV_0962c
    ED98:
    SAAV_0979 (ypfP)
    HO 5096 0412:
    SAEMRSA15_08470
    JH1:
    SaurJH1_1034
    JH9:
    SaurJH9_1015
    JKD6008:
    SAA6008_00972 (ypfP)
    JKD6159:
    SAA6159_00873 (ypfP)
    JSNZ:
    JSNZ_000976
    LGA251:
    SARLGA251_09320 (ugtP)
    M013:
    M013TW_0942
    MRSA252:
    SAR0987
    MSHR1132:
    SAMSHR1132_08640
    MSSA476:
    SAS0886
    Mu3:
    SAHV_1011
    Mu50:
    SAV1017
    MW2:
    MW0898
    RF122:
    SAB0883c
    ST398:
    SAPIG1013
    T0131:
    SAT0131_01052
    TCH60:
    HMPREF0772_12217 (ypfP)
    TW20:
    SATW20_10140 (ugtP)
    USA300_TCH1516:
    USA300HOU_0975
    VC40:
    SAVC_04250

⊟Genome Viewer[edit | edit source]

COL
N315
NCTC8325
Newman
USA300_FPR3757

⊟Alignments[edit | edit source]

  • alignment of orthologues:
    CLUSTAL format alignment by MAFFT L-INS-i (v7.307)


    COL             MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKW
    N315            MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKW
    NCTC8325        MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKW
    Newman          MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKW
    USA300_FPR3757  MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKW
                    ************************************************************

    COL             YINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVL
    N315            YINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVL
    NCTC8325        YINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVL
    Newman          YINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVL
    USA300_FPR3757  YINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVL
                    ************************************************************

    COL             TEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPID
    N315            TEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPID
    NCTC8325        TEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPID
    Newman          TEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPID
    USA300_FPR3757  TEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPID
                    ************************************************************

    COL             NKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGK
    N315            NKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGK
    NCTC8325        NKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGK
    Newman          NKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGK
    USA300_FPR3757  NKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGK
                    ************************************************************

    COL             SKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLN
    N315            SKELKRSLIAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLN
    NCTC8325        SKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLN
    Newman          SKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLN
    USA300_FPR3757  SKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLN
                    ******** ***************************************************

    COL             PAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQT
    N315            PAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQT
    NCTC8325        PAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQT
    Newman          PAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQT
    USA300_FPR3757  PAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQT
                    ************************************************************

    COL             ICRDLLDLIGHSSQPQEIYGKVPLYARFFVK
    N315            ICRDLLDLIGHSSQPQEIYGKVPLYARFFVK
    NCTC8325        ICRDLLDLIGHSSQPQEIYGKVPLYARFFVK
    Newman          ICRDLLDLIGHSSQPQEIYGKVPLYARFFVK
    USA300_FPR3757  ICRDLLDLIGHSSQPQEIYGKVPLYARFFVK
                    *******************************

  1. ↑ M Y Kiriukhin, D V Debabov, D L Shinabarger, F C Neuhaus
    Biosynthesis of the glycolipid anchor in lipoteichoic acid of Staphylococcus aureus RN4220: role of YpfP, the diglucosyldiacylglycerol synthase.
    J Bacteriol: 2001, 183(11);3506-14
    [PubMed:11344159] [WorldCat.org] [DOI] (P p)    Angelika Gründling, Olaf Schneewind
    Genes required for glycolipid synthesis and lipoteichoic acid anchoring in Staphylococcus aureus.
    J Bacteriol: 2007, 189(6);2521-30
    [PubMed:17209021] [WorldCat.org] [DOI] (P p)    Iris Fedtke, Diana Mader, Thomas Kohler, Hermann Moll, Graeme Nicholson, Raja Biswas, Katja Henseler, Friedrich Götz, Ulrich Zähringer, Andreas Peschel
    A Staphylococcus aureus ypfP mutant with strongly reduced lipoteichoic acid (LTA) content: LTA governs bacterial surface properties and autolysin activity.
    Mol Microbiol: 2007, 65(4);1078-91
    [PubMed:17640274] [WorldCat.org] [DOI] (P p)    Won-Sik Yeo, Bohyun Jeong, Nimat Ullah, Majid Ali Shah, Amjad Ali, Kyeong Kyu Kim, Taeok Bae
    Ftsh Sensitizes Methicillin-Resistant Staphylococcus aureus to β-Lactam Antibiotics by Degrading YpfP, a Lipoteichoic Acid Synthesis Enzyme.
    Antibiotics (Basel): 2021, 10(10);
    [PubMed:34680778] [WorldCat.org] [DOI] (P e)    Troy A Burtchett, John C Shook, Laura E Hesse, Philip C Delekta, Robert S Brzozowski, Alhakam Nouri, Alexa J Calas, Catherine M Spanoudis, Prahathees J Eswara, Neal D Hammer
    Crucial Role for Lipoteichoic Acid Assembly in the Metabolic Versatility and Antibiotic Resistance of Staphylococcus aureus.
    Infect Immun: 2023, 91(7);e0055022
    [PubMed:37347167] [WorldCat.org] [DOI] (I p)
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