⊟Summary[edit | edit source]
- pan ID?: SAUPAN003195000
- symbol?: ugtP
- synonym:
- description?: diacylglycerol glucosyltransferase
- diacylglycerol glucosyltransferase
- diacylglycerol beta-glucosyltransferase
- diglucosyl diacylglycerol synthase
- UDP-glucose diacylglycerol glucosyltransferase, putative
- diglucosyldiacylglycerol synthase (LTA membrane anchor synthesis)
- processive diacylglycerol glucosyltransferase
- 1,2-diacylglycerol 3-glucosyltransferase
- monogalactosyldiacylglycerol (MGDG) synthase family protein
- processive diacylglycerol glucosyltransferase YpfP
- UDP-glucose diacylglycerol glucosyltransferase
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 3586499..3587674
- synteny block?: BlockID0022820
- occurrence?: in 100% of 34 strains
ypfP (ugtP) : diacylglycerol glucosyltransferase [1]
Formation of diglucosyl-diacylglycerol on the inner leaflet of the cell membrane by diacylglycerol glucosyltransferase is the first committed step in teichoic acid synthesis. YpfP catalyzes the iterative, processive transfer of glucose from two equivalents of cytosolic UDP-glucose onto a membrane-bound diacylglycerol anchor, providing the platform upon which lipoteichoic acids will eventually be built. This activity is regulated by the membrane protein-processing metalloprotease FtsH which, when activated, degrades YpfP, halting lipoteichoic acid biosynthesis until new YpfP enzyme can be synthesized. Its activity can also be modulated allosterically by CozEa and CozEb proteins although they have a more dramatic role on the activity of the subsequent enzyme in the pathway, diglucosyl-diacylglycerol flippase.
⊟Orthologs[edit | edit source]
⊟Genome Viewer[edit | edit source]
COL | |
N315 | |
NCTC8325 | |
Newman | |
USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKW
N315 MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKW
NCTC8325 MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKW
Newman MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKW
USA300_FPR3757 MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKW
************************************************************
COL YINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVL
N315 YINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVL
NCTC8325 YINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVL
Newman YINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVL
USA300_FPR3757 YINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVL
************************************************************
COL TEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPID
N315 TEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPID
NCTC8325 TEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPID
Newman TEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPID
USA300_FPR3757 TEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPID
************************************************************
COL NKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGK
N315 NKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGK
NCTC8325 NKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGK
Newman NKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGK
USA300_FPR3757 NKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGK
************************************************************
COL SKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLN
N315 SKELKRSLIAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLN
NCTC8325 SKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLN
Newman SKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLN
USA300_FPR3757 SKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLN
******** ***************************************************
COL PAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQT
N315 PAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQT
NCTC8325 PAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQT
Newman PAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQT
USA300_FPR3757 PAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQT
************************************************************
COL ICRDLLDLIGHSSQPQEIYGKVPLYARFFVK
N315 ICRDLLDLIGHSSQPQEIYGKVPLYARFFVK
NCTC8325 ICRDLLDLIGHSSQPQEIYGKVPLYARFFVK
Newman ICRDLLDLIGHSSQPQEIYGKVPLYARFFVK
USA300_FPR3757 ICRDLLDLIGHSSQPQEIYGKVPLYARFFVK
*******************************
- ↑ M Y Kiriukhin, D V Debabov, D L Shinabarger, F C Neuhaus
Biosynthesis of the glycolipid anchor in lipoteichoic acid of Staphylococcus aureus RN4220: role of YpfP, the diglucosyldiacylglycerol synthase.
J Bacteriol: 2001, 183(11);3506-14
[PubMed:11344159] [WorldCat.org] [DOI] (P p)Angelika Gründling, Olaf Schneewind
Genes required for glycolipid synthesis and lipoteichoic acid anchoring in Staphylococcus aureus.
J Bacteriol: 2007, 189(6);2521-30
[PubMed:17209021] [WorldCat.org] [DOI] (P p)Iris Fedtke, Diana Mader, Thomas Kohler, Hermann Moll, Graeme Nicholson, Raja Biswas, Katja Henseler, Friedrich Götz, Ulrich Zähringer, Andreas Peschel
A Staphylococcus aureus ypfP mutant with strongly reduced lipoteichoic acid (LTA) content: LTA governs bacterial surface properties and autolysin activity.
Mol Microbiol: 2007, 65(4);1078-91
[PubMed:17640274] [WorldCat.org] [DOI] (P p)Won-Sik Yeo, Bohyun Jeong, Nimat Ullah, Majid Ali Shah, Amjad Ali, Kyeong Kyu Kim, Taeok Bae
Ftsh Sensitizes Methicillin-Resistant Staphylococcus aureus to β-Lactam Antibiotics by Degrading YpfP, a Lipoteichoic Acid Synthesis Enzyme.
Antibiotics (Basel): 2021, 10(10);
[PubMed:34680778] [WorldCat.org] [DOI] (P e)Troy A Burtchett, John C Shook, Laura E Hesse, Philip C Delekta, Robert S Brzozowski, Alhakam Nouri, Alexa J Calas, Catherine M Spanoudis, Prahathees J Eswara, Neal D Hammer
Crucial Role for Lipoteichoic Acid Assembly in the Metabolic Versatility and Antibiotic Resistance of Staphylococcus aureus.
Infect Immun: 2023, 91(7);e0055022
[PubMed:37347167] [WorldCat.org] [DOI] (I p)