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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1783 [new locus tag: SAUSA300_RS09760 ]
  • pan locus tag?: SAUPAN004758000
  • symbol: hemE
  • pan gene symbol?: hemE
  • synonym:
  • product: uroporphyrinogen decarboxylase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1783 [new locus tag: SAUSA300_RS09760 ]
  • symbol: hemE
  • product: uroporphyrinogen decarboxylase
  • replicon: chromosome
  • strand: -
  • coordinates: 1965116..1966153
  • length: 1038
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTGCATAATAAAAACAATACAATTTTAAAAATGATCAAAGGTGAAGAAACATCACAT
    ACACCTGTTTGGTTTATGCGACAAGCTGGCCGTTCGCAACCAGAATATCGAAAATTGAAA
    GAAAAATATTCACTATTCGATATTACACATCAGCCGGAGTTGTGCGCTTATGTAACACAT
    TTACCAGTTGATAATTATCATACAGATGCAGCAATTTTATACAAAGATATTATGACACCA
    TTAAAGCCAATTGGTGTCGATGTAGAAATTAAATCGGGTATTGGTCCAGTGATTCATAAT
    CCAATCAAAACAATTCAAGATGTTGAGAAACTTTCTCAAATAGACCCCGAACGAGATGTA
    CCATATGTATTAGATACAATTAAACTTTTAACAGAAGAAAAGTTAAATGTGCCGCTAATA
    GGATTTACTGGGGCACCATTTACATTAGCGTCATATATGATTGAAGGCGGACCATCGAAA
    AATTACAATTTTACAAAAGCGATGATGTATAGAGATGAAGCAACATGGTTTGCTTTAATG
    AATCATTTAGTTGATGTATCTGTTAAATATGTAACAGCTCAAGTCGAAGCAGGTGCCGAA
    TTGATTCAAATTTTCGATTCATGGGTAGGTGCATTAAATGTCGAGGATTACAGACGTTAC
    ATTAAACCACATATGATTCGATTAATCAGTGAGGTTAAAGAAAAACATGATGTGCCGGTA
    ATTTTATTCGGTGTAGGTGCCAGTCATTTAATCAATGAATGGAATGATTTACCGATTGAT
    GTATTAGGCCTAGATTGGAGAACGTCTATTAATCAGGCTCAACAATTAGGCGTTACTAAA
    ACATTACAAGGGAATTTAGATCCATCAATTTTATTAGCACCATGGAATGTCATTGAAGAG
    AGATTGAAACCAATATTAGATCAAGGTATGGAGAACGGTAAACACATCTTTAATTTAGGA
    CACGGTGTATTCCCAGAAGTGCAACCAGAGACGTTACGTAAAGTAAGCGAATTCGTACAC
    ACATATACACAAAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1038

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_1783 [new locus tag: SAUSA300_RS09760 ]
  • symbol: HemE
  • description: uroporphyrinogen decarboxylase
  • length: 345
  • theoretical pI: 6.324
  • theoretical MW: 39352.1
  • GRAVY: -0.231304

Function[edit | edit source]

  • reaction:
    EC 4.1.1.37?  ExPASy
    Uroporphyrinogen decarboxylase Uroporphyrinogen III = coproporphyrinogen + 4 CO2
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin uroporphyrinogen decarboxylase (TIGR01464; EC 4.1.1.37; HMM-score: 420.6)
    and 2 more
    methyltransferase, MtaA/CmuA family (TIGR01463; EC 2.1.1.-; HMM-score: 61.8)
    Signal transduction Regulatory functions Protein interactions phosphohistidine phosphatase SixA (TIGR00249; EC 3.1.3.-; HMM-score: 15.4)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, Pigments Tetrapyrroles CPO analysis  Uroporphyrinogen III decarboxylase (EC 4.1.1.37)
    and 1 more
    Experimental tye  Uroporphyrinogen III decarboxylase (EC 4.1.1.37)
  • PFAM:
    Methionine_synt (CL0160) URO-D; Uroporphyrinogen decarboxylase (URO-D) (PF01208; HMM-score: 403.6)
    and 1 more
    TPR (CL0020) COPI_C; Coatomer (COPI) alpha subunit C-terminus (PF06957; HMM-score: 11.2)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006385
    • TAT(Tat/SPI): 0.000484
    • LIPO(Sec/SPII): 0.000852
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MVHNKNNTILKMIKGEETSHTPVWFMRQAGRSQPEYRKLKEKYSLFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPIKTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSKNYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYRRYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQLGVTKTLQGNLDPSILLAPWNVIEERLKPILDQGMENGKHIFNLGHGVFPEVQPETLRKVSEFVHTYTQR

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: PerR* (repression) regulon
    PerR(TF)important in Oxidative stress response; RegPrecise  

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]