PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1793 [new locus tag: SAUSA300_RS09815 ]
• pan locus tag^?: SAUPAN004774000
• symbol: SAUSA300_1793
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1793 [new locus tag: SAUSA300_RS09815 ]
• symbol: SAUSA300_1793
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1976504..1977700
• length: 1197
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913847 NCBI
• RefSeq: YP_494476 NCBI
• BioCyc: see SAUSA300_RS09815
• MicrobesOnline: 1293308 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGTTAAATTTATTCATTGTTCGGATTTACATTTAGATAGTCCTTTCAAATCTAAGAGT
  CATATAAGCCCTAAAATTTTTGAGGATGTTCAAAAAAGTGCTTATGAAAGTTTTAAAAAT
  ATTGTAGATATTGCATTACAACAAGATGTTGATTTTGTAATTATCGCGGGTGATTTATTT
  GATAGTGAAAACAGAACATTACGTGCTGAAATTTTCTTAAAACAGCAATTTGAACGTTTA
  CAAAATGAACAAATCTTTGTTTATGTTTGTCATGGTAATCACGATCCTTTATCATCAAAG
  ATTTCATCAAACTGGCCAGATAATGTTTCTGTATTTTCAAATAAAGTTGAGACGTATGAA
  GCAATTACTAAATCTGGTGAAACAATTTATATTCACGGATTTAGTTATGAAAATAGAGCA
  AGTTATGAGAACAAGATTGATGAATATCCATCAAGTCAAGGCCAAAAAGGCATACATATT
  GGTGTCTTGCATGGTACGTATAGTAAATCTTCAGTTAACGAAAGATATACCGAGTTCATT
  TTAGAAGATTTAAACAGTAAATTGTATCATTATTGGGCTTTAGGTCATATACATGAACGT
  CAACAATTAAGTGATATGCCTGTAATTAACTATTCAGGTAATATTCAAGGTAGACATTTT
  AATGAGCAAGGTGAAAAAGGTTGCTTATTAATCGAGGGTGACCACTTAAAATTAAAGACT
  AAATTTTATCCTACACAGTATATTAGATTTGAAGAAGCAACTATTGAAACGGATAAGACA
  TCTAAGCAAGGTTTATACGAGGTCATTCAAAACTTTAAAGAACAAGTGAGAGAAGAAGGA
  AAAGCCTTTTATCGTTTAACGCTTGTTATTAATAGTGAGACATTAATTTCACCTCAAGAT
  TTATTACAAGTTGAAGAAATGATTACAGATTATGAAGAAAACGAAAATCAATTTGTATAT
  ATTGATGAGTTAAAAATACAATATGCACAAAATGATGAGTCACCTTTAGTTAATGAATTT
  TCAGCGGAATTATTAGTCGATCAAACTGTTTTTGATAAAGCGATGTCAGATTTATATTTA
  AATCCAAGGGCATCTAAGTTCCTAGACGATTATGGAACATTCGACCATACAGCATTAGTT
  AATCGTGCTGAAGAAATATTAAAAGCTGAAATGAGAGGTGAACAAAATGATAATTAA
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  1197
  

⊟Protein

⊟General

• locus tag: SAUSA300_1793 [new locus tag: SAUSA300_RS09815 ]
• symbol: SAUSA300_1793
• description: hypothetical protein
• length: 398
• theoretical pI: 4.63383
• theoretical MW: 46224.1
• GRAVY: -0.586181

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair exonuclease SbcCD, D subunit (TIGR00619; HMM-score: 60.3)
  and 5 more
  metallophosphoesterase, DNA ligase-associated (TIGR04123; HMM-score: 20.7)
  putative phosphoesterase (TIGR03729; HMM-score: 20)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA repair protein (mre11) (TIGR00583; HMM-score: 19.9)
  Unknown function Enzymes of unknown specificity phosphodiesterase, MJ0936 family (TIGR00040; EC 3.1.-.-; HMM-score: 15.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 10.8)
• TheSEED  :
  • DNA repair exonuclease family protein YhaO
  DNA Metabolism DNA repair DNA repair, bacterial  DNA repair exonuclease family protein YhaO
• PFAM:
  Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 53)
  and 3 more
  Metallophos_2; Calcineurin-like phosphoesterase superfamily domain (PF12850; HMM-score: 41)
  no clan defined DUF835; Protein of unknown function (DUF835) (PF05763; HMM-score: 18.5)
  Chelatase (CL0043) CbiK; Cobalt chelatase (CbiK) (PF06180; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001938
  • TAT(Tat/SPI): 0.000183
  • LIPO(Sec/SPII): 0.000916
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159960 NCBI
• RefSeq: YP_494476 NCBI
• UniProt: A0A0H2XFI3 UniProt

⊟Protein sequence

• MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDIALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKISSNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHIGVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGRHFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQVREEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDESPLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMRGEQNDN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: cbf1 < SAUSA300_1792 < SAUSA300_1793

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications