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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_2173 [new locus tag: SAUSA300_RS11995 ]
- pan locus tag?: SAUPAN005669000
- symbol: truA
- pan gene symbol?: truA
- synonym:
- product: tRNA pseudouridine synthase A
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_2173 [new locus tag: SAUSA300_RS11995 ]
- symbol: truA
- product: tRNA pseudouridine synthase A
- replicon: chromosome
- strand: -
- coordinates: 2353630..2354433
- length: 804
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3915365 NCBI
- RefSeq: YP_494808 NCBI
- BioCyc: see SAUSA300_RS11995
- MicrobesOnline: 1293688 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGCGTATATTAGTAGAAATTGCGTATCAAGGAAATAATTTTCTAGGTTTTCAAATTCAA
CAAAATGGTCGAACGGTACAGCAACAATTTGAAAAGCTATTACAACGCATGCATAAAAGA
CATGTGAGAATCCATCCTTCAAGTAGGACTGATAGAGGTGTACATGCGATACAGCAATAC
TTTCATTTCGACACAGAGCTGAATATACCTATGTCACAATGGCAATATGCAATGAACCGA
ACGTTACCTGATGATATTTATGTCAACAATGTAGTTACAGTCGATGACGATTTTCATTGT
CGTTATGATTGTGTAGGTAAACGTTATCGCTACAAAGTATATCAAGCACAACACCGCGAT
CCATTTCAAAGTGGTTTGAAAACATTTATTCCTGAAACGTTGGATTTAGGTAAAATGAAC
AGAGCGGCGCAACAATTCATAGGTACACATGATTTTACGGGATTTTGTTCGCAAAAAACT
GAAGTAGAAAGCAAAGTTAGAACATTATACCAAAGTGAAATCGTAAAGACAGATGATGGC
TTTGATTATATTGTCACTGGATCTGGATTTTTATATAACATGGTGCGTGTCTTAGTTGCA
TTTTTAATAGAAGTTGGAAAAGGGCGACATGAAGTATCTGATGTACCAAAACTTCTTGAA
TCAAAGAATCGTAAGAATGTACCTTTCACTGCACCGGCTGAAGGATTATATTTAGAGAAA
ATTTATTTAGATGAAAATGAGTTATTAAAAGACTTTGGTAACGATATCAAAATACATCGT
AAAAAATCATTACAAAATGACTGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_2173 [new locus tag: SAUSA300_RS11995 ]
- symbol: TruA
- description: tRNA pseudouridine synthase A
- length: 267
- theoretical pI: 8.70635
- theoretical MW: 31279.3
- GRAVY: -0.591386
⊟Function[edit | edit source]
- reaction: EC 5.4.99.12? ExPASytRNA pseudouridine(38-40) synthase tRNA uridine(38-40) = tRNA pseudouridine(38-40)
- TIGRFAM: Protein synthesis tRNA and rRNA base modification tRNA pseudouridine(38-40) synthase (TIGR00071; EC 5.4.99.12; HMM-score: 219.2)and 1 moreradical SAM-linked protein (TIGR03936; HMM-score: 16.8)
- TheSEED :
- tRNA pseudouridine synthase A (EC 4.2.1.70)
RNA Metabolism RNA processing and modification tRNA modification Archaea tRNA pseudouridine synthase A (EC 4.2.1.70)and 2 more - PFAM: PseudoU_synth (CL0649) PseudoU_synth_1; tRNA pseudouridine synthase (PF01416; HMM-score: 138.6)and 1 moreDUF2344; Uncharacterized protein conserved in bacteria (DUF2344) (PF10105; HMM-score: 14.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002749
- TAT(Tat/SPI): 0.000193
- LIPO(Sec/SPII): 0.000467
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRIHPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRYDCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKTEVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKLLESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: truA < SAUSA300_2174 < cbiO < cbiO
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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