PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2469 [new locus tag: SAUSA300_RS13700 ]
• pan locus tag^?: SAUPAN006183000
• symbol: sdaAA
• pan gene symbol^?: sdaAA
• synonym:
• product: L-serine dehydratase, iron-sulfur-dependent, alpha subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2469 [new locus tag: SAUSA300_RS13700 ]
• symbol: sdaAA
• product: L-serine dehydratase, iron-sulfur-dependent, alpha subunit
• replicon: chromosome
• strand: -
• coordinates: 2665406..2666305
• length: 900
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913451 NCBI
• RefSeq: YP_495103 NCBI
• BioCyc: see SAUSA300_RS13700
• MicrobesOnline: 1293984 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTTTGATTCAATTAGAGAGACTATAGATTATGCCGTAGAAAATAATATGTCATTTGCG
  GATATCATGGTTAAAGAAGAAATGGAATTAAGCGGTAAATCACGTGATGAAGTGCGAGCG
  CAAATGAAACAAAATTTAGATGTCATGCGAGACGCAGTAATCAAAGGGACGACAGGTGAT
  GGGGTTGAAAGTGTAACGGGCTACACTGGTCATGATGCTGCTAAACTACGTGATTATAAT
  GAAACACATCATGCTTTGTCTGGATATGAAATGATTGACGCAGTCAAAGGTGCCATTGCA
  ACAAATGAAGTCAATGCTGCGATGGGTATTATTTGTGCAACGCCAACAGCTGGTTCCTCG
  GGTACCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTAACAGAAGAGCAA
  ATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAATGCAAGT
  GTAGCTGGTGCAACAGGTGGCTGCCAAGCTGAAGTTGGTTCAGCATCTGCAATGGCCGCA
  GCTGCAGCAGTAGCTATATTCGGAGGATCACCAGAAGCATCCGGGCACGCTATGGCATTA
  GCGATAAGTAATTTATTAGGTTTAGTTTGTGATCCAGTAGCCGGACTTGTTGAAATTCCT
  TGTGTTATGAGAAATGCAATTGGTTCGGGTAACGCTTTAATTTCAGCAGATTTAGCATTA
  GCAGGTATTGAAAGTAGAATCCCTGTTGATGAAGTTATTGAAGCAATGGATAAGGTTGGT
  CGTAACCTTCCTGCATCATTACGTGAAACTGGATTGGGTGGACTAGCAGGCACACCAACT
  GGCGAAGCAATTAAACGTAAAATCTTTGGCACAGCTGAAGATATGGTTAAAAATAATTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2469 [new locus tag: SAUSA300_RS13700 ]
• symbol: SdaAA
• description: L-serine dehydratase, iron-sulfur-dependent, alpha subunit
• length: 299
• theoretical pI: 4.42838
• theoretical MW: 30971
• GRAVY: 0.100334

⊟Function

• reaction:
  EC 4.3.1.17?  ExPASy
  L-serine ammonia-lyase L-serine = pyruvate + NH₃
• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines L-serine dehydratase, iron-sulfur-dependent, alpha subunit (TIGR00718; EC 4.3.1.17; HMM-score: 374.2)
  Metabolism Energy metabolism Glycolysis/gluconeogenesis L-serine dehydratase, iron-sulfur-dependent, alpha subunit (TIGR00718; EC 4.3.1.17; HMM-score: 374.2)
  and 2 more
  Metabolism Energy metabolism Amino acids and amines L-serine ammonia-lyase (TIGR00720; EC 4.3.1.17; HMM-score: 188.9)
  Metabolism Energy metabolism Glycolysis/gluconeogenesis L-serine ammonia-lyase (TIGR00720; EC 4.3.1.17; HMM-score: 188.9)
• TheSEED  :
  • L-serine dehydratase, alpha subunit (EC 4.3.1.17)
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  L-serine dehydratase, alpha subunit (EC 4.3.1.17)
  and 1 more
  Carbohydrates Central carbohydrate metabolism Pyruvate Alanine Serine Interconversions  L-serine dehydratase, alpha subunit (EC 4.3.1.17)
• PFAM:
  no clan defined SDH_alpha; Serine dehydratase alpha chain (PF03313; HMM-score: 238)
  and 1 more
  T3SS-Chaperone (CL0419) YscO-like; YscO-like protein (PF16789; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008833
  • TAT(Tat/SPI): 0.001752
  • LIPO(Sec/SPII): 0.00055
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162317 NCBI
• RefSeq: YP_495103 NCBI
• UniProt: A0A0H2XIJ0 UniProt

⊟Protein sequence

• MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQNLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATNEVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNASVAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVEIPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLAGTPTGEAIKRKIFGTAEDMVKNN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: sdaAA < sdaAB < SAUSA300_2471

⊟Regulation

• regulator: CcpA regulon

  CcpA

  (TF)

  important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications