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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2429 [new locus tag: NWMN_RS13965 ]
  • pan locus tag?: SAUPAN006183000
  • symbol: sdhA
  • pan gene symbol?: sdaAA
  • synonym:
  • product: L-serine dehydratase, iron-sulfur-dependent, alpha subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2429 [new locus tag: NWMN_RS13965 ]
  • symbol: sdhA
  • product: L-serine dehydratase, iron-sulfur-dependent, alpha subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 2671447..2672346
  • length: 900
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGTTTGATTCAATTAGAGAGACTATAGATTATGCCGTAGAAAATAATATGTCATTTGCG
    GATATCATGGTTAAAGAAGAAATGGAATTAAGCGGTAAATCACGTGATGAAGTGCGAGCG
    CAAATGAAACAAAATTTAGATGTCATGCGAGACGCAGTAATCAAAGGGACGACAGGTGAT
    GGGGTTGAAAGTGTAACGGGCTACACTGGTCATGATGCTGCTAAACTACGTGATTATAAT
    GAAACACATCATGCTTTGTCTGGATATGAAATGATTGACGCAGTCAAAGGTGCCATTGCA
    ACAAATGAAGTCAATGCTGCGATGGGTATTATTTGTGCAACGCCAACAGCTGGTTCCTCG
    GGTACCATTCCCGGTGCACTTTTTAAATTAGAAAAAACACATGATTTAACAGAAGAGCAA
    ATGATTGATTTCTTATTCACTTCAGCATTGTTTGGGCGTGTCGTAGCAAACAATGCAAGT
    GTAGCTGGTGCAACAGGTGGCTGCCAAGCTGAAGTTGGTTCAGCATCTGCAATGGCCGCA
    GCTGCAGCAGTAGCTATATTCGGAGGATCACCAGAAGCATCCGGGCACGCTATGGCATTA
    GCGATAAGTAATTTATTAGGTTTAGTTTGTGATCCAGTAGCCGGACTTGTTGAAATTCCT
    TGTGTTATGAGAAATGCAATTGGTTCGGGTAACGCTTTAATTTCAGCAGATTTAGCATTA
    GCAGGTATTGAAAGTAGAATCCCTGTTGATGAAGTTATTGAAGCAATGGATAAGGTTGGT
    CGTAACCTTCCTGCATCATTACGTGAAACTGGATTGGGTGGACTAGCAGGCACACCAACT
    GGCGAAGCAATTAAACGTAAAATCTTTGGCACAGCTGAAGATATGGTTAAAAATAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2429 [new locus tag: NWMN_RS13965 ]
  • symbol: SdhA
  • description: L-serine dehydratase, iron-sulfur-dependent, alpha subunit
  • length: 299
  • theoretical pI: 4.42838
  • theoretical MW: 30971
  • GRAVY: 0.100334

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines L-serine dehydratase, iron-sulfur-dependent, alpha subunit (TIGR00718; EC 4.3.1.17; HMM-score: 374.2)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis L-serine dehydratase, iron-sulfur-dependent, alpha subunit (TIGR00718; EC 4.3.1.17; HMM-score: 374.2)
    and 2 more
    Metabolism Energy metabolism Amino acids and amines L-serine ammonia-lyase (TIGR00720; EC 4.3.1.17; HMM-score: 188.9)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis L-serine ammonia-lyase (TIGR00720; EC 4.3.1.17; HMM-score: 188.9)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined SDH_alpha; Serine dehydratase alpha chain (PF03313; HMM-score: 238)
    and 1 more
    T3SS-Chaperone (CL0419) YscO-like; YscO-like protein (PF16789; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008833
    • TAT(Tat/SPI): 0.001752
    • LIPO(Sec/SPII): 0.00055
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFDSIRETIDYAVENNMSFADIMVKEEMELSGKSRDEVRAQMKQNLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATNEVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNASVAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVEIPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLAGTPTGEAIKRKIFGTAEDMVKNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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