PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2531 [new locus tag: SAUSA300_RS14045 ]
• pan locus tag^?: SAUPAN006278000
• symbol: SAUSA300_2531
• pan gene symbol^?: —
• synonym:
• product: CocE/NonD family hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2531 [new locus tag: SAUSA300_RS14045 ]
• symbol: SAUSA300_2531
• product: CocE/NonD family hydrolase
• replicon: chromosome
• strand: +
• coordinates: 2731859..2733541
• length: 1683
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914782 NCBI
• RefSeq: YP_495165 NCBI
• BioCyc: see SAUSA300_RS14045
• MicrobesOnline: 1294046 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAACCAACATTTACTAGGAAATCCAAAATTAACTGTAACTCATGTCAATGAAGTTAAA
  GCCGGTATTAACCACATCGTTGTCGACAGTGTTCAATATGGAAATCAAGAAATGATTATG
  GAAAAAGATGTCACTGTGGAAATGCGCGATGGCGAAAAATTATATATTAATATTTTCAGA
  CCAAATAAAGATGGCAAATTCCCTGTAGTTATGTCTGCAGATACTTACGGTAAAGATAAT
  AAGCCTAAAATCACAAATATGGGTGCCCTTTGGCCAACATTAGGTACCATTCCGACATCT
  AGTTTTACACCTGAAGAATCACCAGACCCAGGATTTTGGGTGCCAAATGATTATGTTGTA
  GTTAAAGTTGCATTACGCGGTAGTGACAAATCCAAAGGCGTCTTATCTCCATGGTCAAAA
  AGAGAAGCGGAAGATTATTACGAAGTGATTGAATGGGCAGCAAATCAGTCATGGAGTAAT
  GGAAATATCGGGACAAATGGTGTTTCTTATCTTGCGGTGACTCAATGGTGGGTCGCATCA
  TTAAATCCACCACATTTAAAAGCAATGATTCCTTGGGAAGGCTTAAATGATATGTATAGA
  GAAGTAGCCTTTCACGGAGGTATACCAGATACTGGCTTTTATCGTTTCTGGACTCAAGGT
  ATTTTTGCGAGATGGACAGATAATCCAAATATCGAAGATTTGATTCAAGCACAACAAGAA
  CATCCTCTGTTCGATGATTTTTGGAAACAGCGTCAAGTGCCATTATCACAAATTAAAACA
  CCTCTACTAACATGTGCTAGTTGGTCTACACAAGGTTTGCACAACCGTGGCTCTTTTGAA
  GGATTTAAACAAGCTGCATCTGAAGAAAAATGGCTATATGTGCATGGACGTAAAGAGTGG
  GAAAGTTACTACGCTAGAGAAAATCTCGAACGCCAAAAATCATTCTTTGATTTTTACCTT
  AAAGAAGAAAATAACGATTGGAAAGATACGCCTCATGTCATTTATGAAGTTAGAGATCAA
  TTTTATAAAGGCGAATTCAAATCAGCGTCAGCGTTCCCTTTACCTAACGCAGAATATACA
  CCATTGTATTTGAATGCTGAAAATCACACATTGAATCATGCAAAGATTAGTAGCGCGCAT
  GTCGCACAATATGACTCTGAAGATAAACAACAAGATGTAAGTTTTAAATATACGTTTGAC
  AAAGATACTGAGTTAGTTGGAAACATGAACTTAAAACTATGGGTAAGCACTAAAGACTCA
  GATGATATGGATTTATTTGCAGGTATTAAAAAGTTAGATCGTCGTGGTAATGAAGTTAAC
  TTCCCTGATTTTAATCATATTGAAAATGGTCAAGTAGCTACTGGTTGGTTACGCGTATCA
  CATCGTGAATTAGATCAAGAAAAATCCTCAATCGCGCAACCTTGGCATAAACATGAAACA
  GAATTAAAGTTGTCACAAGATGAGATTGTACCTGTTGAAATCGAATTGTTACCTTCAGGC
  ACGCTATTTAAACAAGGCGAAACATTGGAAGTTGTTGTAAAGGGTAGTGAAATTGTAATT
  GGTAATAGTACTCCTGGCATGAAAACACGTTATGAACATGAAGAAACCGTAAATAAAGGC
  ATGCACATGATTTATACTGGTGGTAAATATGATTCACAATTAATCATTCCTATCGTTAAT
  TGA
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  1683
  

⊟Protein

⊟General

• locus tag: SAUSA300_2531 [new locus tag: SAUSA300_RS14045 ]
• symbol: SAUSA300_2531
• description: CocE/NonD family hydrolase
• length: 560
• theoretical pI: 5.29295
• theoretical MW: 64289.6
• GRAVY: -0.629286

⊟Function

• TIGRFAM:
  Unknown function Enzymes of unknown specificity hydrolase CocE/NonD family protein (TIGR00976; HMM-score: 255.6)
• TheSEED  :
  • Predicted acyl esterase/dipeptidyl-peptidase
• PFAM:
  GBD (CL0202) PepX_C; X-Pro dipeptidyl-peptidase C-terminal non-catalytic domain (PF08530; HMM-score: 162)
  AB_hydrolase (CL0028) Peptidase_S15; X-Pro dipeptidyl-peptidase (S15 family) (PF02129; HMM-score: 142.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00752
  • TAT(Tat/SPI): 0.000258
  • LIPO(Sec/SPII): 0.000704
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161228 NCBI
• RefSeq: YP_495165 NCBI
• UniProt: A0A0H2XGQ4 UniProt

⊟Protein sequence

• MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDVTVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGTIPTSSFTPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAANQSWSNGNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWTQGIFARWTDNPNIEDLIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNRGSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVIYEVRDQFYKGEFKSASAFPLPNAEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKQQDVSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENGQVATGWLRVSHRELDQEKSSIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGETLEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications