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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS00965 [old locus tag: SAUSA300_0184 ]
- pan locus tag?: SAUPAN001021000
- symbol: SAUSA300_RS00965
- pan gene symbol?: argB
- synonym:
- product: acetylglutamate kinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS00965 [old locus tag: SAUSA300_0184 ]
- symbol: SAUSA300_RS00965
- product: acetylglutamate kinase
- replicon: chromosome
- strand: -
- coordinates: 212624..213388
- length: 765
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (212624..213388) NCBI
- BioCyc: SAUSA300_RS00965 BioCyc
- MicrobesOnline: see SAUSA300_0184
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721ATGAAATTTATTGTCATTAAAATTGGTGGCAGTACACTTAGTGACATGCATCCATCAATT
ATTAACAACATTAAGCATTTACGATCAAACAACATCTACCCCATTATCGTTCATGGCGGT
GGCCCATTTATTAATGAAGCATTATCAAACCAGCAAATCGAGCCACACTTTGTTAATGGC
CTAAGAGTGACTGATAAAGCAACCATGACCATTACTAAACACACGCTCATTGCAGACGTT
AACACTGCATTAGTAGCTCAATTTAACCAGCACCAATGTTCTGCAATAGGCTTATGTGGT
TTGGATGCACAGCTGTTTGAAATTACATCTTTTGATCAACAATATGGATATGTCGGTGTT
CCGACCGCTTTAAATAAGGATGCTTTACAGTATTTATGTACTAAATTTGTACCTATCATC
AATTCGATTGGTTTCAATAACCATGATGGAGAATTTTACAATATTAATGCTGACACGCTT
GCCTATTTTATTGCATCATCATTAAAAGCGCCTATTTATGTATTAAGTAATATTGCAGGT
GTACTCATCAATGATGTTGTTATACCTCAATTGCCATTAGTCGATATTCATCAATATATT
GAACATGGTGATATTTATGGAGGTATGATTCCCAAAGTGCTAGATGCCAAAAATGCGATT
GAAAATGGCTGTCCTAAAGTTATCATTGCATCAGGAAACAAGCCAAATATCATTGAATCT
ATTTACAATAATGATTTTGTTGGCACAACAATCCTTAATTCATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS00965 [old locus tag: SAUSA300_0184 ]
- symbol: SAUSA300_RS00965
- description: acetylglutamate kinase
- length: 254
- theoretical pI: 6.21852
- theoretical MW: 27738.7
- GRAVY: 0.183858
⊟Function[edit | edit source]
- reaction: EC 2.7.2.8? ExPASyAcetylglutamate kinase ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate
- TIGRFAM: Amino acid biosynthesis Glutamate family acetylglutamate kinase (TIGR00761; EC 2.7.2.8; HMM-score: 284.1)and 4 moreAmino acid biosynthesis Glutamate family amino-acid N-acetyltransferase (TIGR01890; EC 2.3.1.1; HMM-score: 51.3)Amino acid biosynthesis Glutamate family glutamate 5-kinase (TIGR01027; EC 2.7.2.11; HMM-score: 18.6)Amino acid biosynthesis Glutamate family delta l-pyrroline-5-carboxylate synthetase (TIGR01092; HMM-score: 17.3)Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 14)
- TheSEED: see SAUSA300_0184
- PFAM: no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 137.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.011465
- TAT(Tat/SPI): 0.000175
- LIPO(Sec/SPII): 0.001681
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446591548 NCBI
- RefSeq: WP_000668894 NCBI
- UniProt: see SAUSA300_0184
⊟Protein sequence[edit | edit source]
- MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFINEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLDAQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTLAYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKNAIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: ArgR see SAUSA300_0184
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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