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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS09125 [old locus tag: SAUSA300_1671 ]
  • pan locus tag?: SAUPAN004384000
  • symbol: SAUSA300_RS09125
  • pan gene symbol?: —
  • synonym:
  • product: HAD family hydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS09125 [old locus tag: SAUSA300_1671 ]
  • symbol: SAUSA300_RS09125
  • product: HAD family hydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 1836476..1837606
  • length: 1131
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    GTGAAGAAAATTTTATTTGATGTTGATGGCGTTTTTTTAAGTGAAGAACGTTGTTTCGAT
    GTCTCTGCATTAACGGTATATGAATTATTAATGGATAAGTGTTATTTAGGTCTTCATTCA
    CATATAGATTGGGAAACTTTGACTGATAACGATATACAAGACATTAGGAATCGAATTTTT
    CAAAAAGATAAGATTTTAAACAAATTAAAATCGCTAGGGCTGAATTCAAATTGGGATATG
    TTATTTATCGTTTTTAGTATTCATTTAATTGATATTTTGAAAAAGTTATCACATGATGAA
    ATTGAGGCATTCATGTATCAAGATGAGCCTGTCGAATTGAAGTTGCAAAATATAAGCACA
    AACTTAGCAGACTGTTTTAATTTAAATGAACAACTACCTTTACAATTTTTGGATAATGTA
    AAAGTTGGTAAAAATAATATTTATGCTGCTTTGGAAGAGTTTGCAACAACAGAGTTACAT
    GTTTCGGATGCTACTTTATTTAGTTTGAAGGGTGCATTATGGACGTTAGCGCAAGAAGTT
    TATCAAGAATGGTATTTAGGATCGAAGTTGTATGAAGATGTTGAAAAGAAAATAGCACGA
    ACTACTTTTAAGACAGGTTATATTTATCAAGAAATTATTTTGAGACCAGTAGATGAAGTT
    AAGGTACTTCTGAATGATTTAAAAGGTGCTGGTTTCGAATTAGGTATTGCAACAGGTCGT
    CCTTATACTGAGACTGTTGTGCCATTTGAAAATTTAGGATTGTTACCATATTTTGAAGCT
    GATTTTATTGCAACAGCAAGTGATGTTTTAGAAGCAGAGAATATGTATCCGCAAGCACGA
    CCATTAGGAAAGCCGAATCCTTTTAGTTATATCGCAGCTTTATATGGTAATAATCGCGAT
    AAATATGAATCTTATATCAATAAGCAAGATAACATTGTAAATAAAGATGACGTATTTATA
    GTAGGCGATTCGTTAGCTGACTTATTAAGTGCTCAAAAAATAGGTGCAACGTTTATTGGA
    ACATTAACAGGTTTAAAAGGTAAGGATGCTGCAGGTGAGTTAGAAGCGCATCATGCCGAC
    TATGTTATTAATCATTTAGGTGAACTTAGAGGTGTACTAGATAATTTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1131

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS09125 [old locus tag: SAUSA300_1671 ]
  • symbol: SAUSA300_RS09125
  • description: HAD family hydrolase
  • length: 376
  • theoretical pI: 4.46136
  • theoretical MW: 42658.3
  • GRAVY: -0.119415

⊟Function[edit | edit source]

  • TIGRFAM:
    AHBA synthesis associated protein (TIGR01454; HMM-score: 30.7)
    HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 30.3)
    Metabolism Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 24.8)
    phosphonatase-like hydrolase (TIGR03351; HMM-score: 24.7)
    and 4 more
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 23.3)
    HAD hydrolase, TIGR01548 family (TIGR01548; HMM-score: 17.2)
    beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 17.1)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 11.9)
  • TheSEED: see SAUSA300_1671
  • PFAM:
    HAD (CL0137) Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 58.1)
    HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 47.1)
    and 2 more
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 22.1)
    Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003091
    • TAT(Tat/SPI): 0.000093
    • LIPO(Sec/SPII): 0.000608
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDWETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIEAFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELHVSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVDEVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMYPQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKIGATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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