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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS09855 [old locus tag: SAUSA300_1801 ]
- pan locus tag?: SAUPAN004784000
- symbol: SAUSA300_RS09855
- pan gene symbol?: fumC
- synonym:
- product: class II fumarate hydratase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS09855 [old locus tag: SAUSA300_1801 ]
- symbol: SAUSA300_RS09855
- product: class II fumarate hydratase
- replicon: chromosome
- strand: -
- coordinates: 1984212..1985597
- length: 1386
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1984212..1985597) NCBI
- BioCyc: SAUSA300_RS09855 BioCyc
- MicrobesOnline: see SAUSA300_1801
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1381ATGTCAGTAAGAATTGAACATGATACTTTTGGAGAAATAGAAGTACCTGCAGATAAATAT
TGGGGTGCTCAAACAGAAAGAAGTAAACGTAATTTCCCAGTTGGTAAAGAGCGTATGCCA
ATCGAAGTAGTTTATGGTTTTGCACAACTAAAGCGTGCAGCAGCAATAGCTAATTTTGAT
TTAGGAAAATTAAGCGAGGCAAAGAAAGATGCCATTGTATACGCATGTGATCAAATTTTA
TCAGGTGAATTAGATGAACACTTCCCACTAGTTGTATGGCAAACAGGAAGCGGTACACAA
AGTAATATGAATGTGAACGAAGTAGTAAGTTATGTTGCTAATATGTATTTAAAAGATCAT
CAAAGTGATGAAAGTATCCACCCAAATGATGATGTAAATAAATCTCAAAGTTCGAATGAT
ACATTCCCAACTGCTATGCACGTTGCATTATATCAAGAGGTTGAAACAAAATTAGAACCT
GCATTAAAACTTTTAAGAAATACTTTGAAAGAAAAAGAAGATAAATTTGATTCAATTATT
AAAATTGGTCGTACACATTTACAAGATGCAACGCCGATCAAACTAGGACAAGAGATTAGT
GGCTGGCGTTATATGCTTGACCGTTGTGAAACAATGTTATCTGAATCTAAGAAGCACATT
TTAAATCTTGCCATCGGTGGTACGGCTGTTGGTACTGGTATTAATGCGCATCCTGAATTT
GGTGATAAAGTGGCACATTATATTTCAGAAAATACGGGTTATCCATTTGTATCTTCTGAA
AATAAATTCCACGCACTTACAGCGCATGATGAAGTTGTTCAATTGCATGGAACATTGAAG
GCATTAGCAGGAGACTTAATGAAAATTGCTAATGATGTGAGATGGTTGGCTTCAGGGCCA
CGAGCTGGTTTGGCAGAAATTTCTATCCCTGAAAATGAACCAGGTTCATCAATTATGCCT
GGTAAAGTTAATCCTACACAATGTGAAATGTTAACAATGGTTGCAGTCCAAGTAATGGGT
AATGATACAGTTGTTGGCTTCGCAAGTTCACAAGGTAACTTTGAATTGAATGTTTATAAA
CCAGTTATTATGCATAATACACTACAATCAATTTATCTTTTAGCTGATGGTATGGAAACA
TTTAATAACAATTGTGCAGTGGGCATTGAACCAATCGAAGAGAATATTGATAATTATTTA
AATCAATCATTAATGTTAGTTACTGCATTAAATCCACATATTGGTTATGAAAAAGCAGCT
CAAATTGCTAAGAAAGCCCATAAAGAAGGTTTAACTTTAAAAGAATCTGCAATTCAAACT
GGATATGTTACAGAAGAACAATTTGAAGCATGGATTAAACCAGAAGATATGGTAGATCCT
CATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS09855 [old locus tag: SAUSA300_1801 ]
- symbol: SAUSA300_RS09855
- description: class II fumarate hydratase
- length: 461
- theoretical pI: 4.97681
- theoretical MW: 51107.5
- GRAVY: -0.329718
⊟Function[edit | edit source]
- reaction: EC 4.2.1.2? ExPASyFumarate hydratase (S)-malate = fumarate + H2O
- TIGRFAM: Energy metabolism TCA cycle fumarate hydratase, class II (TIGR00979; EC 4.2.1.2; HMM-score: 751.7)and 4 moreEnergy metabolism Amino acids and amines aspartate ammonia-lyase (TIGR00839; EC 4.3.1.1; HMM-score: 469.7)Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis adenylosuccinate lyase (TIGR00928; EC 4.3.2.2; HMM-score: 88.9)Energy metabolism Other 3-carboxy-cis,cis-muconate cycloisomerase (TIGR02426; EC 5.5.1.2; HMM-score: 73)Amino acid biosynthesis Glutamate family argininosuccinate lyase (TIGR00838; EC 4.3.2.1; HMM-score: 60.3)
- TheSEED: see SAUSA300_1801
- PFAM: no clan defined Lyase_1; Lyase (PF00206; HMM-score: 381)and 4 moreFumaraseC_C; Fumarase C C-terminus (PF10415; HMM-score: 91)DNA_primase_lrg (CL0242) DNA_primase_lrg; Eukaryotic and archaeal DNA primase, large subunit (PF04104; HMM-score: 16)KNTase_C (CL0291) NTase_sub_bind; Nucleotidyltransferase substrate binding protein like (PF08780; HMM-score: 15.4)no clan defined HsbA; Hydrophobic surface binding protein A (PF12296; HMM-score: 14.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.003889
- TAT(Tat/SPI): 0.000959
- LIPO(Sec/SPII): 0.000775
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446038369 NCBI
- RefSeq: WP_000116224 NCBI
- UniProt: see SAUSA300_1801
⊟Protein sequence[edit | edit source]
- MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVVYGFAQLKRAAAIANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSNMNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEPALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCETMLSESKKHILNLAIGGTAVGTGINAHPEFGDKVAHYISENTGYPFVSSENKFHALTAHDEVVQLHGTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMVAVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPIEENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEAWIKPEDMVDPH
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_RS10295 (gatA) glutamyl-tRNA(Gln) amidotransferase subunit A [1] (data from MRSA252) SAUSA300_RS01885 acetyl-CoA acyltransferase [1] (data from MRSA252) SAUSA300_RS02805 50S ribosomal protein L10 [1] (data from MRSA252) SAUSA300_RS02820 DNA-directed RNA polymerase subunit beta [1] (data from MRSA252) SAUSA300_RS02845 elongation factor G [1] (data from MRSA252) SAUSA300_RS03050 phosphate acetyltransferase [1] (data from MRSA252) SAUSA300_RS03480 hypothetical protein [1] (data from MRSA252) SAUSA300_RS03510 transcriptional regulator [1] (data from MRSA252) SAUSA300_RS03530 LysR family transcriptional regulator [1] (data from MRSA252) SAUSA300_RS04080 aldehyde dehydrogenase [1] (data from MRSA252) SAUSA300_RS04560 NADH dehydrogenase [1] (data from MRSA252) SAUSA300_RS04645 NAD-specific glutamate dehydrogenase [1] (data from MRSA252) SAUSA300_RS04670 glucose-6-phosphate isomerase [1] (data from MRSA252) SAUSA300_RS04925 hypothetical protein [1] (data from MRSA252) SAUSA300_RS05350 pyruvate dehydrogenase E1 component subunit alpha [1] (data from MRSA252) SAUSA300_RS06220 30S ribosomal protein S2 [1] (data from MRSA252) SAUSA300_RS08395 molecular chaperone DnaK [1] (data from MRSA252) SAUSA300_RS08950 isocitrate dehydrogenase (NADP(+)) [1] (data from MRSA252) SAUSA300_RS11380 uracil phosphoribosyltransferase [1] (data from MRSA252) SAUSA300_RS12015 50S ribosomal protein L17 [1] (data from MRSA252) SAUSA300_RS12045 adenylate kinase [1] (data from MRSA252) SAUSA300_RS12055 50S ribosomal protein L15 [1] (data from MRSA252) SAUSA300_RS12065 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_RS12140 50S ribosomal protein L23 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)