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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS10180 [old locus tag: SAUSA300_1864 ]
- pan locus tag?: SAUPAN004898000
- symbol: SAUSA300_RS10180
- pan gene symbol?: —
- synonym:
- product: YihY/virulence factor BrkB family protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS10180 [old locus tag: SAUSA300_1864 ]
- symbol: SAUSA300_RS10180
- product: YihY/virulence factor BrkB family protein
- replicon: chromosome
- strand: +
- coordinates: 2024254..2025471
- length: 1218
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2024254..2025471) NCBI
- BioCyc: SAUSA300_RS10180 BioCyc
- MicrobesOnline: see SAUSA300_1864
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201ATGTCGAAAAAGGATCACTCTTCTTCAAAATACCTTAATTCTGTTAAGGAAGCGCAAGAG
GAGTCAAAAAAGAAAAATAAAAGTAATCCCAAAATTGATGTTGATCGTACATATATTGAA
CCTCAACAATTCCAATCTAAGAAACCTAAAAAAGATGATCAGGTTTTCTTCTTATCAAGA
TTAAATAAACCTGCAAAATATAAGAAAGACTCTAATTTCTTATCATATCTCATCTATCGC
ATAGGAAAAGATGATGCCTCAGGACTAGCAGCACAAATGACTTACCATTTCGTACTTGCT
ATGTTCCCTATGTTGCTTTTCCTATTAACATTATTACCATTTTTCAATATTAAGCAGAGT
CAAATTACTAATATGTTAAGCAATGCACCCGCTGAAACATCTACTCTAATTAAGAGTGTA
ATTGGTGATATAACTCAAAACTCCAGTGGTGGCTTATTATCTATCGGTTTGATTTTAGCA
ATTTGGTCAGCTTCAAATGGAATGACTGCAATTATGAATTCTTTCAATGTTGCTTACGAT
GTAGAAGATAGCCGTAATGGAATCGTATTAAAACTACTAAGTGTTGTCTTCACTGTAGTT
ATGGGCGTTGTGTTTGTAGTTGCTCTAGCATTACCAACGCTTGGTTCTGTAATTAGTCAT
TTCCTATTCGGTCCACTTGGATTTGACGAACAAGTGAAATGGATTTTTAACCTTATTAGA
ATTGTGTTACCAATCATTATTATATTTATCATATTTATCGTGTTATATTCGGTTGCACCT
AACGTTAAAACGAAGCTTAAGTCAGTATTACCAGGTGCAGTATTTACTTCAATTATTTGG
TTAGCTGGTTCATTTGGTTTTGGTTGGTATATTTCAAATTTTGGTAACTATTCTAAAACA
TATGGCAGTATCGCGGGTATCATCATTTTGTTACTATGGTTATATATCACAAGTTTTATT
ATAATTGTCGGTGCTGAAATCAATGCAATCATTCATCAGCGTAGTGTAATTAAAGGTAAA
ACACCTGAAGAAGCAGCATTAGAACATGATGACAATAATAAAAATCATTATAATGAGAAC
ACTAGATACGAATATGATGAAGATAGTAATGCAACACATCAACGTACGTATCATGTTGAT
GAACATCCTAGTGATACCTATCCAGAAGATGATAAAAATATAAAAGACAAAATCGTTGAT
AAGCTTAAAAAAGACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS10180 [old locus tag: SAUSA300_1864 ]
- symbol: SAUSA300_RS10180
- description: YihY/virulence factor BrkB family protein
- length: 405
- theoretical pI: 9.26901
- theoretical MW: 45604.4
- GRAVY: 0.0162963
⊟Function[edit | edit source]
- TIGRFAM: Unknown function General YihY family inner membrane protein (TIGR00765; HMM-score: 165.5)and 1 moreTranscription Degradation of RNA inner membrane protein YhjD (TIGR00766; HMM-score: 33.6)
- TheSEED: see SAUSA300_1864
- PFAM: no clan defined Virul_fac_BrkB; Virulence factor BrkB (PF03631; HMM-score: 234.3)and 2 moreDUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 11.5)Oxa1 (CL0376) 60KD_IMP; 60Kd inner membrane protein (PF02096; HMM-score: 10.4)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 6
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.015037
- TAT(Tat/SPI): 0.002293
- LIPO(Sec/SPII): 0.002907
- predicted transmembrane helices (TMHMM): 6
⊟Accession numbers[edit | edit source]
- GI: 445959220 NCBI
- RefSeq: WP_000037075 NCBI
- UniProt: see SAUSA300_1864
⊟Protein sequence[edit | edit source]
- MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQFQSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMFPMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILAIWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVISHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFTSIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQRSVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDKNIKDKIVDKLKKD
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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