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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS10945 [old locus tag: SAUSA300_1991 ]
- pan locus tag?: SAUPAN005279000
- symbol: SAUSA300_RS10945
- pan gene symbol?: agrC
- synonym:
- product: ATP-binding protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS10945 [old locus tag: SAUSA300_1991 ]
- symbol: SAUSA300_RS10945
- product: ATP-binding protein
- replicon: chromosome
- strand: +
- coordinates: 2147721..2148965
- length: 1245
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2147721..2148965) NCBI
- BioCyc: SAUSA300_RS10945 BioCyc
- MicrobesOnline: see SAUSA300_1991
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201ATGATATTAATGTTTACAATACCAGCTATAATTAGTGGTATTAAGTACAGTAAACTTGAT
TATTTTTTCATCATAGTAATTTCGACATTATCGTTATTTCTATTTAAAATGTTTGATAGC
GCGTCCTTAATCATATTAACTTCATTTATTATTATAATGTATTTTGTCAAAATCAAATGG
TATTCTATTTTGTTGATTATGACTTCGCAGATTATTCTATACTGTGCTAACTACATGTAT
ATAGTTATATATGCATATATCACCAAAATTTCTGATAGTATATTTGTAATATTCCCTAGC
TTTTTTGTAGTTTATGTGACTATTAGTATACTATTCTCATATATAATAAATAGAGTTCTC
AAAAAAATTAGCACACCATATCTAATACTAAACAAAGGATTTTTAATAGTTATTTCGACT
ATCTTACTGCTTACTTTTTCATTATTTTTCTTTTATTCACAAATAAACTCGGATGAAGCT
AAAGTAATAAGGCAGTATTCTTTTATTTTTATTGGTATCACTATATTTTTAAGTATATTA
ACATTTGTTATTTCTCAATTTCTCCTTAAAGAGATGAAATATAAACGTAATCAAGAAGAA
ATTGAAACCTATTATGAATATACATTGAAGATTGAAGCTATCAACAACGAAATGCGCAAG
TTCCGTCATGATTATGTCAATATCTTAACGACACTTTCAGAATACATTCGAGAAGATGAC
ATGCCTGGCCTACGTGATTATTTCAATAAAAATATTGTACCTATGAAAGACAATTTACAA
ATGAATGCTATAAAATTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTA
ATTACTGCGAAAATTTTACGTGCACAAGAAATGAATTTTCCGATTAGTATCGAAATACCC
GATGAAGTAAGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGTATTATT
CTTGATAATGCAATTGAGGCATCAACTGAAATTGATGACCCTATCATTCGCGTTGCATTT
ATTGAAAGTGAAAATTCAGTAACGTTTATTGTTATGAATAAATGCGCTGATGATATACCA
CGCATTCATGAATTGTTCCAAGAAAGTTTTTCTACTAAAGGTGAAGGTCGTGGTTTAGGT
CTATCAACTTTAAAAGAAATTGCTGATAATGCAGACAATGTCTTATTAGATACAATTATC
GAAAATGGTTTCTTTATTCAAAAAGTTGAAATTATTAACAACTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS10945 [old locus tag: SAUSA300_1991 ]
- symbol: SAUSA300_RS10945
- description: ATP-binding protein
- length: 414
- theoretical pI: 5.01931
- theoretical MW: 48017.3
- GRAVY: 0.474155
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED: see SAUSA300_1991
- PFAM: His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 66.3)and 3 moreHATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 17.9)GHD (CL0369) Glyco_hydro_30C; Glycosyl hydrolase family 30 beta sandwich domain (PF17189; HMM-score: 14.4)no clan defined SPOB_a; Sensor_kinase_SpoOB-type, alpha-helical domain (PF14689; HMM-score: 13.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 6
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.00145
- TAT(Tat/SPI): 0.000079
- LIPO(Sec/SPII): 0.021614
- predicted transmembrane helices (TMHMM): 6
⊟Accession numbers[edit | edit source]
- GI: see SAUSA300_1991
- RefSeq: WP_001802710 NCBI
- UniProt: see SAUSA300_1991
⊟Protein sequence[edit | edit source]
- MILMFTIPAIISGIKYSKLDYFFIIVISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMPGLRDYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNFPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
SAUSA300_RS11505 (deoA) pyrimidine-nucleoside phosphorylase [1] (data from MRSA252) SAUSA300_RS00585 peptidoglycan-binding protein LysM [1] (data from MRSA252) SAUSA300_RS01155 formate acetyltransferase [1] (data from MRSA252) SAUSA300_RS01940 30S ribosomal protein S6 [1] (data from MRSA252) SAUSA300_RS02025 alkyl hydroperoxide reductase subunit C [1] (data from MRSA252) SAUSA300_RS02575 50S ribosomal protein L25/general stress protein Ctc [1] (data from MRSA252) SAUSA300_RS02800 50S ribosomal protein L1 [1] (data from MRSA252) SAUSA300_RS02805 50S ribosomal protein L10 [1] (data from MRSA252) SAUSA300_RS02810 50S ribosomal protein L7/L12 [1] (data from MRSA252) SAUSA300_RS02825 DNA-directed RNA polymerase subunit beta' [1] (data from MRSA252) SAUSA300_RS02845 elongation factor G [1] (data from MRSA252) SAUSA300_RS02850 elongation factor Tu [1] (data from MRSA252) SAUSA300_RS03480 hypothetical protein [1] (data from MRSA252) SAUSA300_RS04080 aldehyde dehydrogenase [1] (data from MRSA252) SAUSA300_RS04100 enolase [1] (data from MRSA252) SAUSA300_RS04560 NADH dehydrogenase [1] (data from MRSA252) SAUSA300_RS04670 glucose-6-phosphate isomerase [1] (data from MRSA252) SAUSA300_RS04700 hypothetical protein [1] (data from MRSA252) SAUSA300_RS05290 phosphocarrier protein HPr [1] (data from MRSA252) SAUSA300_RS05295 phosphoenolpyruvate--protein phosphotransferase [1] (data from MRSA252) SAUSA300_RS05350 pyruvate dehydrogenase E1 component subunit alpha [1] (data from MRSA252) SAUSA300_RS05355 pyruvate dehydrogenase E1 component subunit beta [1] (data from MRSA252) SAUSA300_RS05360 dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex [1] (data from MRSA252) SAUSA300_RS05365 dihydrolipoyl dehydrogenase [1] (data from MRSA252) SAUSA300_RS05855 cell division protein FtsZ [1] (data from MRSA252) SAUSA300_RS06080 beta-ketoacyl-ACP reductase [1] (data from MRSA252) SAUSA300_RS06135 50S ribosomal protein L19 [1] (data from MRSA252) SAUSA300_RS06160 succinyl-CoA ligase subunit beta [1] (data from MRSA252) SAUSA300_RS06165 succinyl-CoA ligase subunit alpha [1] (data from MRSA252) SAUSA300_RS06220 30S ribosomal protein S2 [1] (data from MRSA252) SAUSA300_RS06230 elongation factor Ts [1] (data from MRSA252) SAUSA300_RS06485 glutamine synthetase [1] (data from MRSA252) SAUSA300_RS07430 DNA-binding protein HU [1] (data from MRSA252) SAUSA300_RS08135 elongation factor P [1] (data from MRSA252) SAUSA300_RS08395 molecular chaperone DnaK [1] (data from MRSA252) SAUSA300_RS08730 50S ribosomal protein L21 [1] (data from MRSA252) SAUSA300_RS08840 trigger factor [1] (data from MRSA252) SAUSA300_RS08875 translation initiation factor IF-3 [1] (data from MRSA252) SAUSA300_RS08950 isocitrate dehydrogenase (NADP(+)) [1] (data from MRSA252) SAUSA300_RS08975 pyruvate kinase [1] (data from MRSA252) SAUSA300_RS09015 universal stress protein [1] (data from MRSA252) SAUSA300_RS09040 universal stress protein UspA [1] (data from MRSA252) SAUSA300_RS09165 formate--tetrahydrofolate ligase [1] (data from MRSA252) SAUSA300_RS09350 proline dehydrogenase [1] (data from MRSA252) SAUSA300_RS10250 non-heme ferritin [1] (data from MRSA252) SAUSA300_RS10900 molecular chaperone GroEL [1] (data from MRSA252) SAUSA300_RS11445 fructose-bisphosphate aldolase [1] (data from MRSA252) SAUSA300_RS11600 glutamine--fructose-6-phosphate aminotransferase [1] (data from MRSA252) SAUSA300_RS11805 Asp23/Gls24 family envelope stress response protein [1] (data from MRSA252) SAUSA300_RS11990 50S ribosomal protein L13 [1] (data from MRSA252) SAUSA300_RS12025 30S ribosomal protein S11 [1] (data from MRSA252) SAUSA300_RS12055 50S ribosomal protein L15 [1] (data from MRSA252) SAUSA300_RS12065 30S ribosomal protein S5 [1] (data from MRSA252) SAUSA300_RS12075 50S ribosomal protein L6 [1] (data from MRSA252) SAUSA300_RS12080 30S ribosomal protein S8 [1] (data from MRSA252) SAUSA300_RS12095 50S ribosomal protein L24 [1] (data from MRSA252) SAUSA300_RS12105 30S ribosomal protein S17 [1] (data from MRSA252) SAUSA300_RS12120 30S ribosomal protein S3 [1] (data from MRSA252) SAUSA300_RS12125 50S ribosomal protein L22 [1] (data from MRSA252) SAUSA300_RS12135 50S ribosomal protein L2 [1] (data from MRSA252) SAUSA300_RS12140 50S ribosomal protein L23 [1] (data from MRSA252) SAUSA300_RS12145 50S ribosomal protein L4 [1] (data from MRSA252) SAUSA300_RS12150 50S ribosomal protein L3 [1] (data from MRSA252) SAUSA300_RS13795 hydroxymethylglutaryl-CoA synthase [1] (data from MRSA252) SAUSA300_RS14105 malate:quinone oxidoreductase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: AgrA, CodY see SAUSA300_1991
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 1.56 1.57 1.58 1.59 1.60 1.61 1.62 1.63 1.64 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)