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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1843 [new locus tag: SA_RS10580 ]
  • pan locus tag?: SAUPAN005279000
  • symbol: agrC
  • pan gene symbol?: agrC
  • synonym:
  • product: accessory gene regulator C

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1843 [new locus tag: SA_RS10580 ]
  • symbol: agrC
  • product: accessory gene regulator C
  • replicon: chromosome
  • strand: +
  • coordinates: 2080353..2081468
  • length: 1116
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGATTCCAACTTTTTCATCTATCATATTTTTATTCTTTAAATTAAAGTACTATGCAATA
    GTAACAATTTTAGTGACCATGATCATAATGTATTTGAGTAATTTTGCAACAGTAGGTTTG
    TTTTTAACTTTAAGAAAATACACTACTGATCCTGCAATACTCTTACCATTATATATACTT
    TCTTTTTCATCAGTTTCATTATTAGCGACATATTTGGTAAGAATATCTCTAAAGAAATTT
    AAAAAATCATACTTATCTTTAAATAAAACTTATATGATTATAATTTCATTCGTATTATTT
    GCAACATTTGCATTTTTTTATATTTATTCTACAAATACAAGTTCAAACGGTGATAGCTTA
    ATTCCATATGCACTTGTATTTATAGGATTAATAATTTTTATCTCTGTTGTAATACTTATA
    ATGTCGCTTTTCACCCTTAAAGAGATGAAATACAAACGAAATCAAGAAGAAATTGAGACC
    TATTACGAATACACATTAAAGATTGAAGCAATTAATAATGAAATGCGCAAGTTCCGTCAT
    GATTATGTCAATATCTTAACGACACTTTCAGAATACATTCGCGAAGATGACATGATTGGT
    CTACGCGCTTATTTCAATAAAAATATTGTACCTATGAAAGATAATTTGCAGATGAATGCT
    ATTAAATTAAATGGTATCGAGAATCTTAAAGTACGTGAGATAAAAGGCTTAATTACTGCG
    AAAATTTTACGTGCACAAGAAATGAATATTCCAATTAGTATCGAAATACCCGATGAAGTA
    AGTAGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGTATTATTCTTGATAAT
    GCAATTGAGGCATCAACTGAAATTGATGACCCTATCATTCGCGTTGCATTTATTGAAAGT
    GAAAATTCAGTAACGTTTATTGTTATGAATAAATGCGCTGATGATATACCACGCATTCAT
    GAATTGTTCCAAGAAAGTTTTTCTACTAAAGGTGAAGGTCGTGGTTTAGGTCTATCAACT
    TTAAAAGAAATTGCTGATAATGCAGACAATGTCTTATTAGATACAATTATCGAAAATGGT
    TTCTTTATTCAAAAAGTTGAAATTATTAACAACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1116

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1843 [new locus tag: SA_RS10580 ]
  • symbol: AgrC
  • description: accessory gene regulator C
  • length: 371
  • theoretical pI: 5.28675
  • theoretical MW: 42544.7
  • GRAVY: 0.357143

Function[edit source | edit]

  • TIGRFAM:
  • TheSEED:  
    Histidine kinase of the competence regulon ComD
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 66.6)
    and 6 more
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 18.2)
    ABC-2 (CL0181) ABC2_membrane_2; ABC-2 family transporter protein (PF12679; HMM-score: 15.3)
    GHD (CL0369) Glyco_hydro_30C; Glycosyl hydrolase family 30 beta sandwich domain (PF17189; HMM-score: 14.7)
    no clan definedSPOB_a; Sensor_kinase_SpoOB-type, alpha-helical domain (PF14689; HMM-score: 13.8)
    NfeD-like (CL0252) NfeD; NfeD-like C-terminal, partner-binding (PF01957; HMM-score: 9.8)
    no clan definedDUF1772; Domain of unknown function (DUF1772) (PF08592; HMM-score: 8.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SA0033(aadD)kanamycin nucleotidyltransferase  [1] (data from MRSA252)
    SA0366(ahpC)alkyl hydroperoxide reductase  [1] (data from MRSA252)
    SA1984(asp23)alkaline shock protein 23  [1] (data from MRSA252)
    SA1517(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    SA1409(dnaK)molecular chaperone DnaK  [1] (data from MRSA252)
    SA0731(eno)phosphopyruvate hydratase  [1] (data from MRSA252)
    SA1074(fabG)3-oxoacyl-ACP reductase  [1] (data from MRSA252)
    SA1927(fbaA)fructose-bisphosphate aldolase  [1] (data from MRSA252)
    SA1553(fhs)formate--tetrahydrofolate ligase  [1] (data from MRSA252)
    SA1029(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    SA0505(fus)elongation factor G  [1] (data from MRSA252)
    SA0727(gap)glyceraldehyde-3-phosphate dehydrogenase  [1] (data from MRSA252)
    SA1959(glmS)glucosamine--fructose-6-phosphate aminotransferase  [1] (data from MRSA252)
    SA1150(glnA)glutamine-ammonia ligase  [1] (data from MRSA252)
    SA1836(groEL)molecular chaperone GroEL  [1] (data from MRSA252)
    SA1305(hu)DNA-binding protein II  [1] (data from MRSA252)
    SA1504(infC)translation initiation factor IF-3  [1] (data from MRSA252)
    SA2400(mqo2)malate:quinone oxidoreductase  [1] (data from MRSA252)
    SA2334(mvaS)3-hydroxy-3-methylglutaryl-CoA synthase  [1] (data from MRSA252)
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    SA0944(pdhB)pyruvate dehydrogenase E1 component subunit beta  [1] (data from MRSA252)
    SA0945(pdhC)branched-chain alpha-keto acid dehydrogenase E2 subunit  [1] (data from MRSA252)
    SA0946(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SA1938(pdp)pyrimidine-nucleoside phosphorylase  [1] (data from MRSA252)
    SA0218(pflB)formate acetyltransferase  [1] (data from MRSA252)
    SA0823(pgi)glucose-6-phosphate isomerase  [1] (data from MRSA252)
    SA0934(ptsH)phosphocarrier protein HPr  [1] (data from MRSA252)
    SA0935(ptsI)phosphoenolpyruvate-protein phosphatase  [1] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [1] (data from MRSA252)
    SA0496(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SA2044(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    SA2047(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SA2046(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    SA2033(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    SA0497(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    SA0498(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    SA2017(rplM)50S ribosomal protein L13  [1] (data from MRSA252)
    SA2029(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SA1084(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SA1473(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    SA2042(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    SA2045(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    SA2036(rplX)50S ribosomal protein L24  [1] (data from MRSA252)
    SA0459(rplY)50S ribosomal protein L25  [1] (data from MRSA252)
    SA0501(rpoC)DNA-directed RNA polymerase subunit beta'  [1] (data from MRSA252)
    SA1099(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SA2041(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SA0352(rpsF)30S ribosomal protein S6  [1] (data from MRSA252)
    SA2034(rpsH)30S ribosomal protein S8  [1] (data from MRSA252)
    SA2024(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    SA2038(rpsQ)30S ribosomal protein S17  [1] (data from MRSA252)
    SA0107(spa)immunoglobulin G binding protein A  [1] (data from MRSA252)
    SA1088(sucC)succinyl-CoA synthetase subunit beta  [1] (data from MRSA252)
    SA1089(sucD)succinyl-CoA synthetase subunit alpha  [1] (data from MRSA252)
    SA1499(tig)trigger factor  [1] (data from MRSA252)
    SA1100(tsf)elongation factor Ts  [1] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [1] (data from MRSA252)
    SA0618hypothetical protein  [1] (data from MRSA252)
    SA0802hypothetical protein  [1] (data from MRSA252)
    SA0829hypothetical protein  [1] (data from MRSA252)
    SA1359elongation factor P  [1] (data from MRSA252)
    SA1528hypothetical protein  [1] (data from MRSA252)
    SA1532hypothetical protein  [1] (data from MRSA252)
    SA1585hypothetical protein  [1] (data from MRSA252)
    SA1709hypothetical protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000886
    • TAT(Tat/SPI): 0.000131
    • LIPO(Sec/SPII): 0.001516
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIPTFSSIIFLFFKLKYYAIVTILVTMIIMYLSNFATVGLFLTLRKYTTDPAILLPLYILSFSSVSLLATYLVRISLKKFKKSYLSLNKTYMIIISFVLFATFAFFYIYSTNTSSNGDSLIPYALVFIGLIIFISVVILIMSLFTLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTTLSEYIREDDMIGLRAYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • regulators: AgrA (activation) regulon, CodY (repression) regulon
    AgrA(TF)important in Virulence, Quorum sensing; RegPrecise  
    CodY(TF)important in Amino acid metabolism; RegPrecise  

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 1.56 1.57 1.58 1.59 1.60 1.61 1.62 1.63 1.64 1.65 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]

Elizabeth A George Cisar, Edward Geisinger, Tom W Muir, Richard P Novick
Symmetric signalling within asymmetric dimers of the Staphylococcus aureus receptor histidine kinase AgrC.
Mol. Microbiol.: 2009, 74(1);44-57
[PubMed:19708918] [WorldCat.org] [DOI] (I p)