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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2025 [new locus tag: SACOL_RS10575 ]
  • pan locus tag?: SAUPAN005279000
  • symbol: argC2
  • pan gene symbol?: agrC
  • synonym:
  • product: accessory gene regulator protein C

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2025 [new locus tag: SACOL_RS10575 ]
  • symbol: argC2
  • product: accessory gene regulator protein C
  • replicon: chromosome
  • strand: +
  • coordinates: 2083869..2085161
  • length: 1293
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    GTGGAATTATTAAATAGTTATAATTTTGTTTTATTCGTATTAACTCAAATGATATTAATG
    TTTACAATACCAGCTATAATTAGTGGTATTAAGTACAGTAAACTTGATTATTTTTTCATC
    ATAGTAATTTCGACATTATCGTTATTTCTATTTAAAATGTTTGATAGCGCGTCCTTAATC
    ATATTAACTTCATTTATTATTATAATGTATTTTGTCAAAATCAAATGGTATTCTATTTTG
    TTGATTATGACTTCGCAGATTATTCTATACTGTGCTAACTACATGTATATAGTTATATAT
    GCATATATCACCAAAATTTCTGATAGTATATTTGTAATATTCCCTAGCTTTTTTGTAGTT
    TATGTGACTATTAGTATACTATTCTCATATATAATAAATAGAGTTCTCAAAAAAATTAGC
    ACACCATATCTAATACTAAACAAAGGATTTTTAATAGTTATTTCGACTATCTTACTGCTT
    ACTTTTTCATTATTTTTCTTTTATTCACAAATAAACTCGGATGAAGCTAAAGTAATAAGG
    CAGTATTCTTTTATTTTTATTGGTATCACTATATTTTTAAGTATATTAACATTTGTTATT
    TCTCAATTTCTCCTTAAAGAGATGAAATATAAACGTAATCAAGAAGAAATTGAAACCTAT
    TATGAATATACATTGAAGATTGAAGCTATCAACAACGAAATGCGCAAGTTCCGTCATGAT
    TATGTCAATATCTTAACGCCACTTTCAGAATACATTCGAGAAGATGACATGCCTGGCCTA
    CGTGATTATTTCAATAAAAATATTGTACCTATGAAAGACAATTTACAAATGAATGCTATA
    AAATTAAATGGTATCGAGAATCTTAAAGTACGTGAAATTAAAGGCTTAATTACTGCGAAA
    ATTTTACGTGCACAAGAAATGAATATTCCGATTAGTATCGAAATACCCGATGAAGTAAGT
    AGCATTAACTTGAATATGATCGATTTAAGTCGCAGTATTGGTATTATTCTTGATAATGCA
    ATTGAGGCATCAACTGAAATTGATGACCCTATCATTCGCGTTGCATTTATTGAAAGTGAA
    AATTCAGTAACGTTTATTGTTATGAATAAATGCGCTGATGATATACCACGCATTCATGAA
    TTGTTCCAAGAAAGTTTTTCTACTAAAGGTGAAGGTCGTGGTTTAGGTCTATCAACTTTA
    AAAGAAATTGCTGATAATGCAGACAATGTCTTATTAGATACAATTATCGAAAATGGTTTC
    TTTATTCAAAAAGTTGAAATTATTAACAACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1293

⊟Protein[edit | edit source]

Protein Data Bank: 4BXI

⊟General[edit | edit source]

  • locus tag: SACOL2025 [new locus tag: SACOL_RS10575 ]
  • symbol: ArgC2
  • description: accessory gene regulator protein C
  • length: 430
  • theoretical pI: 4.92642
  • theoretical MW: 49892.6
  • GRAVY: 0.491628

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Histidine kinase of the competence regulon ComD
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c_5; GHKL domain (PF14501; HMM-score: 66.2)
    and 2 more
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 17.8)
    GHD (CL0369) Glyco_hydro_30C; Glycosyl hydrolase family 30 beta sandwich domain (PF17189; HMM-score: 14.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005191
    • TAT(Tat/SPI): 0.000262
    • LIPO(Sec/SPII): 0.037738
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MELLNSYNFVLFVLTQMILMFTIPAIISGIKYSKLDYFFIIVISTLSLFLFKMFDSASLIILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFFVVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEAKVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEMRKFRHDYVNILTPLSEYIREDDMPGLRDYFNKNIVPMKDNLQMNAIKLNGIENLKVREIKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDPIIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNADNVLLDTIIENGFFIQKVEIINN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SACOL0452(ahpC)alkyl hydroperoxide reductase subunit C  [3] (data from MRSA252)
    SACOL1637(dnaK)molecular chaperone DnaK  [3] (data from MRSA252)
    SACOL1587(efp)elongation factor P  [3] (data from MRSA252)
    SACOL0842(eno)phosphopyruvate hydratase  [3] (data from MRSA252)
    SACOL1245(fabG1)3-oxoacyl-ACP reductase  [3] (data from MRSA252)
    SACOL2117(fbaA)fructose-bisphosphate aldolase  [3] (data from MRSA252)
    SACOL1329(femC)glutamine synthetase  [3] (data from MRSA252)
    SACOL1782(fhs)formate--tetrahydrofolate ligase  [3] (data from MRSA252)
    SACOL1199(ftsZ)cell division protein FtsZ  [3] (data from MRSA252)
    SACOL0593(fusA)elongation factor G  [3] (data from MRSA252)
    SACOL0838(gapA1)glyceraldehyde 3-phosphate dehydrogenase  [3] (data from MRSA252)
    SACOL2145(glmS)glucosamine--fructose-6-phosphate aminotransferase  [3] (data from MRSA252)
    SACOL2016(groEL)chaperonin GroEL  [3] (data from MRSA252)
    SACOL1513(hup)DNA-binding protein HU  [3] (data from MRSA252)
    SACOL1741(icd)isocitrate dehydrogenase  [3] (data from MRSA252)
    SACOL1727(infC)translation initiation factor IF-3  [3] (data from MRSA252)
    SACOL2623(mqo2)malate:quinone oxidoreductase  [3] (data from MRSA252)
    SACOL1102(pdhA)pyruvate dehydrogenase complex E1 component subunit alpha  [3] (data from MRSA252)
    SACOL1103(pdhB)pyruvate dehydrogenase complex E1 component subunit beta  [3] (data from MRSA252)
    SACOL1104(pdhC)branched-chain alpha-keto acid dehydrogenase E2  [3] (data from MRSA252)
    SACOL1105(pdhD)dihydrolipoamide dehydrogenase  [3] (data from MRSA252)
    SACOL2128(pdp)pyrimidine-nucleoside phosphorylase  [3] (data from MRSA252)
    SACOL0204(pflB)formate acetyltransferase  [3] (data from MRSA252)
    SACOL0966(pgi)glucose-6-phosphate isomerase  [3] (data from MRSA252)
    SACOL1091(ptsH)phosphocarrier protein HPr  [3] (data from MRSA252)
    SACOL1092(ptsI)phosphoenolpyruvate-protein phosphotransferase  [3] (data from MRSA252)
    SACOL1816(putA)proline dehydrogenase  [3] (data from MRSA252)
    SACOL1745(pyk)pyruvate kinase  [3] (data from MRSA252)
    SACOL0584(rplA)50S ribosomal protein L1  [3] (data from MRSA252)
    SACOL2236(rplB)50S ribosomal protein L2  [3] (data from MRSA252)
    SACOL2239(rplC)50S ribosomal protein L3  [3] (data from MRSA252)
    SACOL2238(rplD)50S ribosomal protein L4  [3] (data from MRSA252)
    SACOL2224(rplF)50S ribosomal protein L6  [3] (data from MRSA252)
    SACOL0585(rplJ)50S ribosomal protein L10  [3] (data from MRSA252)
    SACOL0586(rplL)50S ribosomal protein L7/L12  [3] (data from MRSA252)
    SACOL2207(rplM)50S ribosomal protein L13  [3] (data from MRSA252)
    SACOL2220(rplO)50S ribosomal protein L15  [3] (data from MRSA252)
    SACOL1257(rplS)50S ribosomal protein L19  [3] (data from MRSA252)
    SACOL1702(rplU)50S ribosomal protein L21  [3] (data from MRSA252)
    SACOL2234(rplV)50S ribosomal protein L22  [3] (data from MRSA252)
    SACOL2237(rplW)50S ribosomal protein L23  [3] (data from MRSA252)
    SACOL2228(rplX)50S ribosomal protein L24  [3] (data from MRSA252)
    SACOL0545(rplY)50S ribosomal protein L25/general stress protein Ctc  [3] (data from MRSA252)
    SACOL0589(rpoC)DNA-directed RNA polymerase subunit beta'  [3] (data from MRSA252)
    SACOL1274(rpsB)30S ribosomal protein S2  [3] (data from MRSA252)
    SACOL2233(rpsC)30S ribosomal protein S3  [3] (data from MRSA252)
    SACOL2222(rpsE)30S ribosomal protein S5  [3] (data from MRSA252)
    SACOL0437(rpsF)30S ribosomal protein S6  [3] (data from MRSA252)
    SACOL2225(rpsH)30S ribosomal protein S8  [3] (data from MRSA252)
    SACOL2214(rpsK)30S ribosomal protein S11  [3] (data from MRSA252)
    SACOL2230(rpsQ)30S ribosomal protein S17  [3] (data from MRSA252)
    SACOL0095(spa)immunoglobulin G binding protein A precursor  [3] (data from MRSA252)
    SACOL1262(sucC)succinyl-CoA synthetase subunit beta  [3] (data from MRSA252)
    SACOL1263(sucD)succinyl-CoA synthetase subunit alpha  [3] (data from MRSA252)
    SACOL1722(tig)trigger factor  [3] (data from MRSA252)
    SACOL1276(tsf)elongation factor Ts  [3] (data from MRSA252)
    SACOL0594(tuf)elongation factor Tu  [3] (data from MRSA252)
    SACOL0721hypothetical protein  [3] (data from MRSA252)
    SACOL0944NADH dehydrogenase  [3] (data from MRSA252)
    SACOL0973fumarylacetoacetate hydrolase  [3] (data from MRSA252)
    SACOL1753universal stress protein  [3] (data from MRSA252)
    SACOL1759universal stress protein  [3] (data from MRSA252)
    SACOL1952ferritins family protein  [3] (data from MRSA252)
    SACOL2173alkaline shock protein 23  [3] (data from MRSA252)
    SACOL2561hydroxymethylglutaryl-CoA synthase  [3] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: AgrA (activation) regulon, CodY (repression) regulon
    AgrA(TF)important in Virulence, Quorum sensing; transcription unit transferred from N315 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 3.24 3.25 3.26 3.27 3.28 3.29 3.30 3.31 3.32 3.33 3.34 3.35 3.36 3.37 3.38 3.39 3.40 3.41 3.42 3.43 3.44 3.45 3.46 3.47 3.48 3.49 3.50 3.51 3.52 3.53 3.54 3.55 3.56 3.57 3.58 3.59 3.60 3.61 3.62 3.63 3.64 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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