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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2023 [new locus tag: SACOL_RS10565 ]
  • pan locus tag?: SAUPAN005275000
  • symbol: agrB
  • pan gene symbol?: agrB
  • synonym:
  • product: accessory gene regulator protein B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2023 [new locus tag: SACOL_RS10565 ]
  • symbol: agrB
  • product: accessory gene regulator protein B
  • replicon: chromosome
  • strand: +
  • coordinates: 2083138..2083707
  • length: 570
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    TTGAATTATTTTGATAATAAAATTGACCAGTTTGCCACGTATCTTCAAAAGAGAAATAAC
    TTAGATCATATTCAATTTTTGCAAGTACGATTAGGGATGCAGGTCTTAGCTAAAAATATA
    GGTAAATTAATTGTTATGTATACTATTGCCTATATTTTAAACATTTTTCTGTTTACGTTA
    ATTACGAATTTAACATTTTATTTAATAAGAAGACATGCACATGGTGCACATGCACCTTCT
    TCTTTTTGGTGTTATGTAGAAAGTATTATACTATTTATACTTTTACCTTTAGTAATAGTA
    AATTTTCATATTAACTTTTTAATTATGATTATTTTAACAGTTATTTCTTTAGGTGTAATC
    TCAGTATATGCTCCTGCAGCAACTAAAAAGAAGCCCATTCCTGTGCGACTTATTAAACGA
    AAAAAATATTATGCGATTATTGTTAGTTTAACCCTTTTCATTATCACACTTATCATCAAA
    GAGCCATTTGCCCAATTCATTCAATTAGGCATCATAATAGAAGCTATTACATTATTACCT
    ATTTTCTTTATTAAGGAGGACTTAAAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    570

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2023 [new locus tag: SACOL_RS10565 ]
  • symbol: AgrB
  • description: accessory gene regulator protein B
  • length: 189
  • theoretical pI: 10.3336
  • theoretical MW: 21929.5
  • GRAVY: 0.827513

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.-.-?  ExPASy
  • TIGRFAM:
    cxxc_20_cxxc protein (TIGR04104; HMM-score: 6.7)
  • TheSEED  :
    • Accessory gene regulator B
  • PFAM:
    no clan defined AgrB; Accessory gene regulator B (PF04647; HMM-score: 114.5)
    and 1 more
    DUF4400; Domain of unknown function (DUF4400) (PF14348; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003149
    • TAT(Tat/SPI): 0.000096
    • LIPO(Sec/SPII): 0.033947
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLIVMYTIAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESIILFILLPLVIVNFHINFLIMIILTVISLGVISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTLFIITLIIKEPFAQFIQLGIIIEAITLLPIFFIKEDLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: AgrA (activation) regulon, CodY (repression) regulon
    AgrA(TF)important in Virulence, Quorum sensing; compare RegPrecise for N315 
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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