PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS11720 [old locus tag: SAUSA300_2128 ]
• pan locus tag^?: SAUPAN005557000
• symbol: SAUSA300_RS11720
• pan gene symbol^?: sdrM
• synonym:
• product: MFS transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS11720 [old locus tag: SAUSA300_2128 ]
• symbol: SAUSA300_RS11720
• product: MFS transporter
• replicon: chromosome
• strand: -
• coordinates: 2301579..2302922
• length: 1344
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (2301579..2302922) NCBI
• BioCyc: SAUSA300_RS11720 BioCyc
• MicrobesOnline: see SAUSA300_2128

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGCGATTAAAGTCAATCATAACTGTTATAGCTTTAATATTAATTATGTTTATGTCAGCG
  ATTGAATCATCTATTATTTCGCTTGCATTACCTACAATTAAACAAGATTTAAATGCAGGA
  AATTTAATTTCATTAATTTTCACAGCGTATTTTATCGCATTAGTAATTGCCAATCCTATA
  GTGGGCGAATTACTTTCGAGATTTAAAATTATTTATGTGGCAATTGCAGGATTACTTTTA
  TTTAGTATAGGTAGTTTTATGTGTGGTTTAAGTACTAATTTTACTATGCTAATTATATCA
  CGCGTGATACAAGGTTTTGGATCAGGTGTATTGATGTCATTATCGCAAATTGTTCCAAAG
  TTAGCATTTGAAATTCCGTTACGATATAAAATTATGGGCATAGTTGGCAGTGTTTGGGGT
  ATTTCGAGTATCATTGGGCCACTTCTAGGTGGTGGTATATTAGAATTTGCAACATGGCAT
  TGGTTATTCTACATAAATATACCGATTGCGATCATTGCCATTATATTAGTTATATGGACA
  TTTCATTTTCCAGAGGAAGAAACAGTTGCTAAATCAAAATTTGATACTAAAGGGCTCACG
  CTATTTTATGTATTTATTGGATTGATTATGTTTGCTTTATTAAACCAACAGCTGTTACTT
  TTAAATTTCTTGAGTTTTATCTTAGCTATCGTTGTGGCAATGTGTTTATTTAAAGTAGAA
  AAACATGTTTCTTCACCATTTTTACCTGTGGTTGAATTTAATCGTTCGATTACTTTAGTT
  TTTATAACTGACCTTCTAACAGCTATTTGTTTAATGGGATTCAATTTATATATTCCAGTC
  TACCTTCAAGAACAACTAGGATTATCTCCATTGCAAAGTGGATTGGTTATTTTTCCTTTA
  TCTGTAGCTTGGATTACATTGAATTTTAATTTACATCGAATTGAAGCAAAACTATCAAGG
  AAAGTTATTTACTTACTATCATTTACATTGCTACTAGTAAGTAGTATTATCATTTCATTT
  GGTATTAAATTGCCGGTACTTATAGCATTTGTGTTAATTTTGGCAGGATTAAGTTTTGGA
  TATATTTATACGAAAGATAGTGTGATTGTCCAAGAGGAAACTAGCCCATTACAAATGAAG
  AAAATGATGTCATTTTATGGATTAACTAAAAATCTTGGGGCATCAATAGGTTCAACAATA
  ATGGGATATCTTTATGCGATACAATCAGGAATCTTTGGTCCAAACTTACACAATGTGTTA
  AGTGCTGTTGCTGTAATTAGCATTGGGCTTATTGTTTTATGGGTCGTATTTTTTAAAGAA
  CAGTCATCTCAATCAAAAGAATAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1344
  

⊟Protein

⊟General

• locus tag: SAUSA300_RS11720 [old locus tag: SAUSA300_2128 ]
• symbol: SAUSA300_RS11720
• description: MFS transporter
• length: 447
• theoretical pI: 9.4822
• theoretical MW: 49409.4
• GRAVY: 1.08523

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 160.6)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 160.6)
  and 8 more
  multidrug resistance protein (TIGR00880; HMM-score: 72.1)
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 58.9)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 52.3)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 38.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 31.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 29.6)
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 24.6)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 8.2)
• TheSEED: see SAUSA300_2128
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 183.3)
  and 2 more
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 28)
  TRI12; Fungal trichothecene efflux pump (TRI12) (PF06609; HMM-score: 23.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 14
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 29 aa
  • SP(Sec/SPI): 0.481061
  • TAT(Tat/SPI): 0.000733
  • LIPO(Sec/SPII): 0.150295
  • Cleavage Site: CS pos: 29-30. SLA-LP. Pr: 0.2959
• predicted transmembrane helices (TMHMM): 14

⊟Accession numbers

• GI: 447160250 NCBI
• RefSeq: WP_001237506 NCBI
• UniProt: see SAUSA300_2128

⊟Protein sequence

• MRLKSIITVIALILIMFMSAIESSIISLALPTIKQDLNAGNLISLIFTAYFIALVIANPIVGELLSRFKIIYVAIAGLLLFSIGSFMCGLSTNFTMLIISRVIQGFGSGVLMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWHWLFYINIPIAIIAIILVIWTFHFPEEETVAKSKFDTKGLTLFYVFIGLIMFALLNQQLLLLNFLSFILAIVVAMCLFKVEKHVSSPFLPVVEFNRSITLVFITDLLTAICLMGFNLYIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHRIEAKLSRKVIYLLSFTLLLVSSIIISFGIKLPVLIAFVLILAGLSFGYIYTKDSVIVQEETSPLQMKKMMSFYGLTKNLGASIGSTIMGYLYAIQSGIFGPNLHNVLSAVAVISIGLIVLWVVFFKEQSSQSKE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications