From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS13005 [old locus tag: SAUSA300_2355 ]
  • pan locus tag?: SAUPAN005944000
  • symbol: SAUSA300_RS13005
  • pan gene symbol?:
  • synonym:
  • product: cystatin-like fold lipoprotein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS13005 [old locus tag: SAUSA300_2355 ]
  • symbol: SAUSA300_RS13005
  • product: cystatin-like fold lipoprotein
  • replicon: chromosome
  • strand: -
  • coordinates: 2531490..2531849
  • length: 360
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    TTGAAGCGATTTGTGGCGACGGTATTATTATTACTAGTCTTTATATCAGGATGTGGTAAT
    GATAAATATGTGAAAGAAATAGATGAAGCAGTTAAAATTCAAAATCAAAAACAAGAACAA
    CTTGCCAAAAAAGGCAACGGTGATCGTGTTGATCATTTTGAACGCAAAGATGCTAATATT
    TATGTCTATGATAAGGATAAAATTATCATTTTAGCTTATAAACCTTTGAGTAATGATGAA
    GTGCATTATTATGCATATGATTTTAGTGATAAACGTGTATCATATAAGCAAGATTTTGAT
    TCGAGACGATATTATCAACAACATGATGCGGATTATCATGAAGAAAATATGACGAACTAG
    60
    120
    180
    240
    300
    360

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_RS13005 [old locus tag: SAUSA300_2355 ]
  • symbol: SAUSA300_RS13005
  • description: cystatin-like fold lipoprotein
  • length: 119
  • theoretical pI: 6.51715
  • theoretical MW: 14213.8
  • GRAVY: -0.9

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED: see SAUSA300_2355
  • PFAM:
    NTF2 (CL0051) DUF4467; Domain of unknown function with cystatin-like fold (DUF4467) (PF14729; HMM-score: 124.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.87
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP:
  • SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
    • SP(Sec/SPI): 0.000417
    • TAT(Tat/SPI): 0.000084
    • LIPO(Sec/SPII): 0.999126
    • Cleavage Site: CS pos: 17-18. ISG-CG. Pr: 0.9999
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKKGNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDEVHYYAYDFSDKRVSYKQDFDSRRYYQQHDADYHEENMTN

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]