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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03200 [old locus tag: SA0553 ]
  • pan locus tag?: SAUPAN002442000
  • symbol: SA_RS03200
  • pan gene symbol?: —
  • synonym:
  • product: transposase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03200 [old locus tag: SA0553 ]
  • symbol: SA_RS03200
  • product: transposase
  • replicon: chromosome
  • strand: +
  • coordinates: 646693..648087
  • length: 1395
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (646693..648087) NCBI
  • BioCyc: G1G21-628 BioCyc
  • MicrobesOnline: see SA0553

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAGTATTGACATGTATTTAGACAGATCTCGAAACCAAGCTTCAAGTGTGGGGAATTTG
    AGTCAAACAATGAATTCAAATTATGATGCGTTGGAAAAAGCAATTACTCAATTTATCAAT
    GATGATGCGCTTAAAGGGAAAGCGTATACGTCAGCTAAGCAATTTTTTAGTACGGTGTTA
    ATTCCATTATCAACAAGTATGAAAACATTGAGTGATTTAACGAAGCAAGCTTGCGATAAT
    TTTGTGTCACGTTATACGAGTGAGGTTGATAGCATATCTTTAAAAGAATCAGAGCTTGAA
    GAAGATATCAGATCATTAAGTCAACAAATTACGCGATATGAAAATTTAACTAACAATTTG
    AAAAAGCATGCTTCCGATAATCAGCAAGCCATTTCATCGAACCAACAAATAATACAAACA
    TTAGGTCAACAAAAACATGAATTAGAAGAGAAACTACGTAAATTACGTGAGTTTAATCAA
    AAATCACCAGAAATATTTAAAGAAGTTGAAGAATTTCAAAAAATTGTCCAACAAGGACTT
    ACCCAAGCGCAGAATTTTTGGAACTTTTCAACAAATCAATTTAATATACCTTCAGGTAAA
    GAACTTGATTGGGCTAAAGCAAGTCATGAAAAATATTTGAAAGTTGCTATGGGGAAAATT
    GAACATAAAGCAGAGAAAGAAACTTTAAATAAAGCAGACTTTGCTGTTATAAAGGCATAT
    GCCAAAGAACATCCAGAAGACGATATCCCGAAAAGTATAATGAAATATATAAATGACAAT
    AAAGATAGTATTAAACGAGATATAGGATTAGATATTACGTCAACACTTTTAGAGCAAGGC
    GGTATAAATGCAAGTAAATTCGGTGTATTTATCAATACAGCAGGTGGAGTGAAAGGCCCA
    GCCGGTCCAAATTCATTTGTGGAAGTCAAACGTACATCAGGTAATGTGTTTATAGAAAAT
    GGTAGTAAATTTGCAAAAGGCGGAAAATATCTAGGGAAAGGTGTTGCAGGTGTAGGATTT
    GGTATAGGTATGTATGATGATCTTGCAAATGATAATAAAACATTTGGAGAGGCATTGTCG
    CATAATGGTATGACGCTTGCAGCTGGATCTGTTGCAGCATTTGGTGTAGCGGCTTTAGTA
    AGTAATCCAGTCGGTTGGGCTGTGGTAGGTATTTTAACAGCAGGTATTATTGGATCTACT
    GTTTCTGATTATCTTTATCAAAATAATTATTTTGGCATAAAAGATAAAGTGGACTGGGCA
    GGACATAAAATAGACGAGGGTATTGATGCTGTAAACAAAACTGCAGAAAAAGCTGTAGAT
    AGTGTTGGTAATGCCATTGGTGATGCCACAAAAAGTATTAGTAATCATATTAATCCTATG
    AAATGGAGTTGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1395

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS03200 [old locus tag: SA0553 ]
  • symbol: SA_RS03200
  • description: transposase
  • length: 464
  • theoretical pI: 6.73714
  • theoretical MW: 50976.9
  • GRAVY: -0.449353

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 14.5)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 14.5)
    SH3 domain protein (TIGR04211; HMM-score: 14.4)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 13.7)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 13.7)
    two transmembrane protein (TIGR04527; HMM-score: 12.5)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 12.2)
    and 2 more
    Cellular processes Cellular processes Pathogenesis virulence factor Mce family protein (TIGR00996; HMM-score: 11.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 4.1)
  • TheSEED: see SA0553
  • PFAM:
    no clan defined LXG; LXG domain of WXG superfamily (PF04740; HMM-score: 50.5)
    and 33 more
    Laminin_II; Laminin Domain II (PF06009; HMM-score: 17.2)
    Tropomyosin-lke (CL0452) Tropomyosin_1; Tropomyosin like (PF12718; HMM-score: 17.2)
    no clan defined DegS; Sensor protein DegS (PF05384; HMM-score: 16.4)
    Ferritin (CL0044) DUF892; Domain of unknown function (DUF892) (PF05974; HMM-score: 16)
    no clan defined DUF3450; Protein of unknown function (DUF3450) (PF11932; HMM-score: 16)
    BCLiA (CL0551) APG6; Autophagy protein Apg6 (PF04111; HMM-score: 14.7)
    no clan defined DUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 13.5)
    DUF812; Protein of unknown function (DUF812) (PF05667; HMM-score: 13.4)
    TPR_MLP1_2; TPR/MLP1/MLP2-like protein (PF07926; HMM-score: 13.3)
    Fe_bilin_red; Ferredoxin-dependent bilin reductase (PF05996; HMM-score: 13.2)
    CALCOCO1; Calcium binding and coiled-coil domain (CALCOCO1) like (PF07888; HMM-score: 13.2)
    CorA; CorA-like Mg2+ transporter protein (PF01544; HMM-score: 13.1)
    GCN5L1; GCN5-like protein 1 (GCN5L1) (PF06320; HMM-score: 11.6)
    TMF_DNA_bd; TATA element modulatory factor 1 DNA binding (PF12329; HMM-score: 10.9)
    CemA; CemA family (PF03040; HMM-score: 10.3)
    Laminin_I; Laminin Domain I (PF06008; HMM-score: 10)
    Mce4_CUP1; Cholesterol uptake porter CUP1 of Mce4, putative (PF11887; HMM-score: 10)
    DUF4200; Domain of unknown function (DUF4200) (PF13863; HMM-score: 10)
    Exonuc_VII_L; Exonuclease VII, large subunit (PF02601; HMM-score: 9.8)
    GOLD-like (CL0521) GOLD_2; Golgi-dynamics membrane-trafficking (PF13897; HMM-score: 9.7)
    Reo_sigma (CL0326) Reo_sigmaC; Reovirus sigma C capsid protein (PF04582; HMM-score: 9.4)
    no clan defined Prominin; Prominin (PF05478; HMM-score: 9.1)
    DUF948; Bacterial protein of unknown function (DUF948) (PF06103; HMM-score: 8.9)
    TMPIT; TMPIT-like protein (PF07851; HMM-score: 8.9)
    SlyX; SlyX (PF04102; HMM-score: 8.8)
    DUF2570; Protein of unknown function (DUF2570) (PF10828; HMM-score: 8.2)
    DUF2935; Domain of unknown function (DUF2935) (PF11155; HMM-score: 8.1)
    V_ATPase_I; V-type ATPase 116kDa subunit family (PF01496; HMM-score: 7.8)
    DUF2353; Uncharacterized coiled-coil protein (DUF2353) (PF09789; HMM-score: 7.8)
    DUF5082; Domain of unknown function (DUF5082) (PF16888; HMM-score: 7.2)
    Fzo_mitofusin; fzo-like conserved region (PF04799; HMM-score: 6.7)
    Prefoldin (CL0200) Prefoldin; Prefoldin subunit (PF02996; HMM-score: 6.5)
    no clan defined Jnk-SapK_ap_N; JNK_SAPK-associated protein-1 (PF09744; HMM-score: 6.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 2
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006641
    • TAT(Tat/SPI): 0.001543
    • LIPO(Sec/SPII): 0.000945
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDALKGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEEDIRSLSQQITRYENLTNNLKKHASDNQQAISSNQQIIQTLGQQKHELEEKLRKLREFNQKSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMGKIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSIMKYINDNKDSIKRDIGLDITSTLLEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKGVAGVGFGIGMYDDLANDNKTFGEALSHNGMTLAAGSVAAFGVAALVSNPVGWAVVGILTAGIIGSTVSDYLYQNNYFGIKDKVDWAGHKIDEGIDAVNKTAEKAVDSVGNAIGDATKSISNHINPMKWSW

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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