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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS03525 [old locus tag: SA0614 ]
  • pan locus tag?: SAUPAN002538000
  • symbol: SA_RS03525
  • pan gene symbol?: graR
  • synonym:
  • product: DNA-binding response regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS03525 [old locus tag: SA0614 ]
  • symbol: SA_RS03525
  • product: DNA-binding response regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 708245..708919
  • length: 675
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (708245..708919) NCBI
  • BioCyc: SA_RS03525 BioCyc
  • MicrobesOnline: see SA0614

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCAAATACTACTAGTAGAAGATGACAATACTTTGTTTCAAGAATTGAAAAAAGAATTA
    GAACAATGGGATTTTAATGTTGCTGGTATTGAAGATTTCGGCAAAGTAATGGATACATTT
    GAAAGTTTTAATCCTGAAATTGTTATATTGGATGTTCAATTACCTAAATATGATGGGTTT
    TATTGGTGCAGAAAAATGAGAGAAGTTTCCAACGTACCAATATTATTTTTATCATCTCGT
    GATAATCCAATGGATCAAGTGATGAGTATGGAACTTGGCGCAGATGATTATATGCAAAAA
    CCTTTCTATACCAATGTATTAATTGCTAAATTACAAGCGATTTATCGTCGTGTCTATGAG
    TTTACAGCTGAAGAAAAACGTACATTGACTTGGCAAGATGCTGTCGTTGATCTATCAAAA
    GATAGTATACAAAAAGGTGACGATACGATTTTTCTATCCAAAACAGAAATGATTATATTA
    GAAATTCTTATTACCAAAAAAAATCAAATCGTTTCGAGAGATACAATTATCACTGCATTA
    TGGGATGATGAAGCATTTGTTAGTGATAATACGTTAACAGTAAATGTGAATCGTTTACGA
    AAAAAATTATCTGAAATTAGTATGGATAGTGCAATCGAAACAAAAGTAGGAAAAGGATAT
    ATGGCTCATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    675

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS03525 [old locus tag: SA0614 ]
  • symbol: SA_RS03525
  • description: DNA-binding response regulator
  • length: 224
  • theoretical pI: 4.35637
  • theoretical MW: 26065.7
  • GRAVY: -0.257589

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 145.1)
    Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 145.1)
    Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 117.2)
    and 7 more
    proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 115.5)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 44.8)
    Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 44.8)
    Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 44.8)
    Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 44.2)
    Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 37.9)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 19.1)
  • TheSEED: see SA0614
  • PFAM:
    CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 76.7)
    HTH (CL0123) Trans_reg_C; Transcriptional regulatory protein, C terminal (PF00486; HMM-score: 76.2)
    and 1 more
    POTRA (CL0191) POTRA_1; POTRA domain, FtsQ-type (PF08478; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by GraR*, response regulator important in cationic antimicrobial peptide (CAMP) resistance: in NCTC8325

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001883
    • TAT(Tat/SPI): 0.000104
    • LIPO(Sec/SPII): 0.00034
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFNPEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPFYTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDDTIFLSKTEMIILEILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGKGYMAHE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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