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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS06825 [old locus tag: SA1200 ]
  • pan locus tag?: SAUPAN003763000
  • symbol: SA_RS06825
  • pan gene symbol?: trpG
  • synonym:
  • product: glutamine amidotransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS06825 [old locus tag: SA1200 ]
  • symbol: SA_RS06825
  • product: glutamine amidotransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1373968..1374534
  • length: 567
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1373968..1374534) NCBI
  • BioCyc: SA_RS06825 BioCyc
  • MicrobesOnline: see SA1200

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGATCTTAGTTGTAGATAATTATGATTCCTTTACATATAACCTAGTGGATATTGTTGCT
    CAACATACTGACGTCATTGTTCAATATCCTGATGATGATAATGTGCTGAATCAATCGGTG
    GACGCTGTTATTATATCTCCTGGTCCAGGGCATCCATTAGACGATCAACAGTTAATGAAA
    ATCATATCAACCTATCAACACAAACCCATTTTAGGTATTTGCCTAGGTGCTCAGGCACTG
    ACTTGTTATTACGGTGGAGAAGTCATTAAAGGCGACAAGGTTATGCACGGAAAAGTTGAT
    ACACTAAAGGTTATATCGCATCATCAACATCTGTTATATCAAGATATACCAGAACAGTTT
    TCAATTATGAGATATCATTCATTAATAAGTAACCCTGACAATTTTCCAGAAGAATTGAAA
    ATTACTGGACGTACCGAAGATTGTATACAGTCATTTGAGCATAAAGAAAGACCGCATTAC
    GGTATTCAGTACCATCCTGAATCATTTGCTACAGACTATGGTGTCAAAATAATTACAAAT
    TTCATTAATCTAGTGAAGGAAGGATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    567

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS06825 [old locus tag: SA1200 ]
  • symbol: SA_RS06825
  • description: glutamine amidotransferase
  • length: 188
  • theoretical pI: 5.18231
  • theoretical MW: 21369.1
  • GRAVY: -0.25266

⊟Function[edit | edit source]

  • TIGRFAM:
    glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase (TIGR00566; HMM-score: 151.3)
    aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 126.8)
    and 5 more
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 115.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 92.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 50.1)
    Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 28.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 17.6)
  • TheSEED: see SA1200
  • PFAM:
    Glutaminase_I (CL0014) GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 126)
    and 3 more
    Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 35.6)
    GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 16)
    KaiA_RbsU (CL0637) KaiA; KaiA C-terminal domain (PF07688; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007604
    • TAT(Tat/SPI): 0.000165
    • LIPO(Sec/SPII): 0.000607
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVIISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTLKVISHHQHLLYQDIPEQFSIMRYHSLISNPDNFPEELKITGRTEDCIQSFEHKERPHYGIQYHPESFATDYGVKIITNFINLVKEG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SA_RS0290550S ribosomal protein L11  [1] (data from MRSA252)
    SA_RS02960elongation factor Tu  [1] (data from MRSA252)
    SA_RS04140aldehyde dehydrogenase  [1] (data from MRSA252)
    SA_RS04160enolase  [1] (data from MRSA252)
    SA_RS04785beta-ketoacyl-[acyl-carrier-protein] synthase II  [1] (data from MRSA252)
    SA_RS0622530S ribosomal protein S2  [1] (data from MRSA252)
    SA_RS06690transketolase  [1] (data from MRSA252)
    SA_RS080505'-methylthioadenosine/S-adenosylhomocysteine nucleosidase  [1] (data from MRSA252)
    SA_RS08505aldehyde dehydrogenase  [1] (data from MRSA252)
    SA_RS08545isocitrate dehydrogenase (NADP(+))  [1] (data from MRSA252)
    SA_RS11245glutamine--fructose-6-phosphate aminotransferase  [1] (data from MRSA252)
    SA_RS1160030S ribosomal protein S9  [1] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: T-box(Trp), CodY see SA1200

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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