PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS06965 [old locus tag: SA1226 ]
• pan locus tag^?: SAUPAN003807000
• symbol: SA_RS06965
• pan gene symbol^?: asd
• synonym:
• product: aspartate-semialdehyde dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS06965 [old locus tag: SA1226 ]
• symbol: SA_RS06965
• product: aspartate-semialdehyde dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 1401012..1402001
• length: 990
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1401012..1402001) NCBI
• BioCyc: G1G21-1406 BioCyc
• MicrobesOnline: see SA1226

⊟Phenotype

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⊟DNA sequence

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  ATGACAAAGTTAGCAGTTGTGGGTGCAACAGGATTAGTAGGCACAAAAATGTTGGAGACA
  TTAAATCGTAAAAATATTCCTTTCGATGAATTAGTATTATTTTCATCAGCACGTTCTGCA
  GGGCAAGAAGTTGAATTTCAAGAAAAAACATATACAGTACAAGAATTAACTGATGCTCGT
  GCTAGTGAACATTTCGATTATGTATTAATGAGTGCTGGTGGCGGTACAAGCGAACACTTT
  GCCCCACTCTTTGAAAAAGCTGGTGCAATCGTTATAGACAATTCAAGTCAATGGCGTATG
  GCAGAAGATATTGATTTAATCGTTCCGGAAGTCAATGAACCTACATTTACAAGAGGTATC
  ATTGCCAATCCAAACTGCTCTACGATTCAATCTGTTGTGCCATTAAAAGTATTGCAAGAT
  GCTTATGGTTTAAAACGAGTGGCATATACAACATATCAAGCTGTATCAGGTTCAGGGATG
  AAAGGTAAGAAAGATTTAGCTGAAGGTGTAAATGGTAAAGCACCAGAAGCATATCCACAT
  CCAATTTATAATAATGTGTTACCGCATATTGATGTGTTTTTAGAAAACGGATATACAAAA
  GAAGAACAAAAAATGATTGATGAGACGAGAAAAATTTTAAATGCGCCAGACTTAAAAGTA
  ACAGCAACATGCGCACGTGTGCCTGTTCAAGATAGTCATAGTGTTGAAATTGATGTAACG
  CTTGACAAAGAAACGACAGCAGAAGATATTAAAGCGTTATTTGATCAAGATGACCGCGTT
  GTTTTAGTAGACAATCCAGAGAACAATGAATATCCAATGGCAATCAATTCCACTAATAAA
  GATGAAGTGTTTGTTGGCCGTATACGTAGAGATGATTCATTAGAAAATACTTTCCATGTA
  TGGTGTACATCAGACAATTTATTAAAAGGTGCTGCATTAAATGCTGTACAAGTATTGGAA
  CAAGTTATGCGTTTAAAAGGAGCGAATTAA
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⊟Protein

⊟General

• locus tag: SA_RS06965 [old locus tag: SA1226 ]
• symbol: SA_RS06965
• description: aspartate-semialdehyde dehydrogenase
• length: 329
• theoretical pI: 4.60472
• theoretical MW: 36354.8
• GRAVY: -0.302432

⊟Function

• reaction:
  EC 1.2.1.11?  ExPASy
  Aspartate-semialdehyde dehydrogenase L-aspartate 4-semialdehyde + phosphate + NADP⁺ = L-4-aspartyl phosphate + NADPH
• TIGRFAM:
  Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01296; EC 1.2.1.11; HMM-score: 379)
  and 9 more
  Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR00978; EC 1.2.1.11; HMM-score: 126.4)
  Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01745; EC 1.2.1.11; HMM-score: 83.5)
  Metabolism Amino acid biosynthesis Glutamate family N-acetyl-gamma-glutamyl-phosphate reductase (TIGR01850; EC 1.2.1.38; HMM-score: 59.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine erythrose-4-phosphate dehydrogenase (TIGR01532; EC 1.2.1.72; HMM-score: 30)
  Metabolism Energy metabolism Glycolysis/gluconeogenesis glyceraldehyde-3-phosphate dehydrogenase, type I (TIGR01534; EC 1.2.1.-; HMM-score: 27.6)
  Metabolism Amino acid biosynthesis Glutamate family N-acetyl-gamma-glutamyl-phosphate reductase (TIGR01851; EC 1.2.1.38; HMM-score: 27.6)
  glyceraldehyde-3-phosphate dehydrogenase, type II (TIGR01546; EC 1.2.1.59; HMM-score: 16.5)
  Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 15.5)
  Genetic information processing Mobile and extrachromosomal element functions Plasmid functions integrating conjugative element protein, PFL_4703 family (TIGR03746; HMM-score: 13.7)
• TheSEED: see SA1226
• PFAM:
  GADPH_aa-bio_dh (CL0139) Semialdhyde_dhC; Semialdehyde dehydrogenase, dimerisation domain (PF02774; HMM-score: 154.4)
  and 6 more
  NADP_Rossmann (CL0063) Semialdhyde_dh; Semialdehyde dehydrogenase, NAD binding domain (PF01118; HMM-score: 99.8)
  GADPH_aa-bio_dh (CL0139) Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain (PF02800; HMM-score: 30.2)
  NADP_Rossmann (CL0063) Ldh_1_N; lactate/malate dehydrogenase, NAD binding domain (PF00056; HMM-score: 17.6)
  Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 16.4)
  no clan defined TGT_C1; C1 domain of tRNA-guanine transglycosylase dimerisation (PF14809; HMM-score: 13.2)
  DUF2895; Protein of unknown function (DUF2895) (PF11444; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.029399
  • TAT(Tat/SPI): 0.000712
  • LIPO(Sec/SPII): 0.004181
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446085668 NCBI
• RefSeq: WP_000163523 NCBI
• UniProt: see SA1226

⊟Protein sequence

• MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEVEFQEKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAEDIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGMKGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDLKVTATCARVPVQDSHSVEIDVTLDKETTAEDIKALFDQDDRVVLVDNPENNEYPMAINSTNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: L-box, CodY see SA1226

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications