PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS08700 [old locus tag: SA1544 ]
• pan locus tag^?: SAUPAN004378000
• symbol: SA_RS08700
• pan gene symbol^?: —
• synonym:
• product: alanine--glyoxylate aminotransferase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS08700 [old locus tag: SA1544 ]
• symbol: SA_RS08700
• product: alanine--glyoxylate aminotransferase family protein
• replicon: chromosome
• strand: +
• coordinates: 1764642..1765799
• length: 1158
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1764642..1765799) NCBI
• BioCyc: SA_RS08700 BioCyc
• MicrobesOnline: see SA1544

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATT
  ATGAGAGAAATTCAAGCACCTATGGTTGGTCATCGTTCTAAAGATTTTGAAGACATCGCA
  CAACAAGCATTTCAAGGTCTAAAGCCAATATTTGGGAGTCAAAATGATGTACTTATTTTA
  ACATCTAGCGGTACAAGCGTCTTGGAGGCTAGTATGTTGAACATTGTAAACCCTGAAGAT
  CACTTCGTTGTCATTGTTTCAGGTGCCTTTGGTAACCGATTTAAACAAATTGCACAAACT
  TATTACAAAAATGTGCACATTTATGACGTAACATGGGGAGAAGCTGTAGATGTTAAAGAT
  TTCATCAATTACCTTTCAACTTTAGATGTTGAAGTTAAAGCAGTATTTAGTCAATATTGC
  GAAACATCTACGACAGTGCTACACCCTATTCACGAGTTAGGAAATGCCATCAATCAATTT
  AATAGTAATATTTATTTTGTAGTTGACGGCGTAAGTTGCATTGGTGCTGTTGATGTTGAC
  ATTAACAAAGATAAAATTGATGTACTTGTTTCTGGTAGTCAAAAAGCAATTATGTTACCT
  CCAGGATTAGCTTTTGTAGCTTATAGCCACCGTGCAAAAGAACGTTTCAAAGAAGTAACT
  ACGCCAAAATTTTATCTAGACTTAAATAAATACATTTCGTCACAAGCTGACAATTCTACA
  CCGTTCACACCAAATGTGTCTTTATTTAGAGGTGTAAATGCATACGTTGAAACCGTAAAA
  GCAGAAGGTTTCAATCACGTAATAGCACGACACTATGCAATTAGAAATGCATTAAGAAGC
  GCCTTAAAAGCATTAGATTTAACTTTATTAGTCAATGATAAAGATGCATCTCCAACGGTT
  ACAGCATTCAAACCTAATACAAATGAAGTGAAAATAATCAAAGATGAACTTAAAAATCGC
  TTTAAAATAACAATTGCTGGTGGTCAAGGCCATCTTAAAGGTCAAATTTTAAGAATTGGT
  CATATGGGGAAAATTAGTCCTTTCGATATTTTATCGGTAGTATCTGCTTTAGAAATTATT
  TTAACTGAACACCGTAAAGTTAACTATATCGGTAAAGGTATATCAAAATATATGGAGGTT
  ATTCATGAAGCAATTTAA
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⊟Protein

⊟General

• locus tag: SA_RS08700 [old locus tag: SA1544 ]
• symbol: SA_RS08700
• description: alanine--glyoxylate aminotransferase family protein
• length: 385
• theoretical pI: 7.46524
• theoretical MW: 42770.8
• GRAVY: -0.0174026

⊟Function

• TIGRFAM:
  2-aminoethylphosphonate aminotransferase (TIGR03301; EC 2.6.1.-; HMM-score: 170.6)
  and 9 more
  Metabolism Central intermediary metabolism Phosphorus compounds 2-aminoethylphosphonate--pyruvate transaminase (TIGR02326; EC 2.6.1.37; HMM-score: 123.7)
  Metabolism Amino acid biosynthesis Serine family phosphoserine transaminase (TIGR01364; EC 2.6.1.52; HMM-score: 38.1)
  cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 32.3)
  Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 30.1)
  Genetic information processing DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 22.3)
  cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 17.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 16.4)
  Genetic information processing Protein synthesis tRNA and rRNA base modification cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 14.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 14.4)
• TheSEED: see SA1544
• PFAM:
  PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 141.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.015789
  • TAT(Tat/SPI): 0.001061
  • LIPO(Sec/SPII): 0.000712
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446213565 NCBI
• RefSeq: WP_000291420 NCBI
• UniProt: see SA1544

⊟Protein sequence

• MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAFQGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYYKNVHIYDVTWGEAVDVKDFINYLSTLDVEVKAVFSQYCETSTTVLHPIHELGNAINQFNSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKEVTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRNALRSALKALDLTLLVNDKDASPTVTAFKPNTNEVKIIKDELKNRFKITIAGGQGHLKGQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SA1544

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications