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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS10845 [old locus tag: SA1885 ]
  • pan locus tag?: SAUPAN005354000
  • symbol: SA_RS10845
  • pan gene symbol?: cshA
  • synonym:
  • product: DEAD/DEAH box family ATP-dependent RNA helicase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS10845 [old locus tag: SA1885 ]
  • symbol: SA_RS10845
  • product: DEAD/DEAH box family ATP-dependent RNA helicase
  • replicon: chromosome
  • strand: -
  • coordinates: 2135902..2137422
  • length: 1521
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2135902..2137422) NCBI
  • BioCyc: SA_RS10845 BioCyc
  • MicrobesOnline: see SA1885

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    TTGCAAAATTTTAAAGAACTAGGGATTTCGGATAATACGGTTCAGTCACTTGAATCAATG
    GGATTTAAAGAGCCGACACCTATCCAAAAAGACAGTATCCCTTATGCGTTACAAGGAATT
    GATATCCTTGGGCAAGCTCAAACCGGTACAGGTAAAACAGGAGCATTCGGTATTCCTTTA
    ATTGAGAAAGTAGTAGGGAAACAAGGGGTTCAATCGTTGATTTTAGCACCTACAAGAGAA
    TTGGCAATGCAGGTAGCTGAACAATTAAGAGAATTTAGCCGTGGACAAGGTGTCCAAGTT
    GTTACTGTATTCGGTGGTATGCCTATCGAACGCCAAATTAAAGCCTTGAAAAAAGGCCCA
    CAAATCGTAGTCGGAACACCTGGGCGTGTTATCGACCATTTAAATCGTCGCACATTAAAA
    ACGGACGGAATTCATACTTTGATTTTAGATGAAGCTGATGAAATGATGAATATGGGATTC
    ATCGATGATATGAGATTTATTATGGATAAAATTCCAGCAGTACAACGTCAAACAATGTTG
    TTCTCAGCTACAATGCCTAAAGCAATCCAAGCTTTAGTACAACAATTTATGAAATCACCA
    AAAATCATTAAGACAATGAATAATGAAATGTCTGATCCACAAATCGAAGAATTCTATACA
    ATTGTTAAAGAATTAGAGAAATTTGATACATTTACAAATTTCCTAGATGTTCATCAACCT
    GAATTAGCAATCGTATTCGGACGTACAAAACGTCGTGTTGATGAATTAACAAGTGCTTTG
    ATTTCTAAAGGATATAAAGCTGAAGGCTTACATGGTGATATTACACAAGCGAAACGTTTA
    GAAGTATTAAAGAAATTTAAAAATGACCAAATTAATATTTTAGTCGCTACTGATGTAGCA
    GCAAGAGGACTAGATATTTCTGGTGTGAGTCATGTTTATAACTTTGATATACCTCAAGAT
    ACTGAAAGCTATACACACCGTATTGGTCGTACGGGTCGTGCTGGTAAAGAAGGTATCGCT
    GTAACGTTTGTTAATCCAATCGAAATGGATTATATCAGACAAATTGAAGATGCAAACGGT
    AGAAAAATGAGTGCACTTCGTCCACCTCATCGTAAAGAAGTACTTCAAGCACGTGAAGAT
    GACATCAAAGAAAAAGTTGAAAACTGGATGTCTAAAGAGTCAGAATCACGCTTGAAACGC
    ATTTCTACAGAGTTGTTAAATGAATATAACGATGTTGATTTAGTTGCTGCACTTTTACAA
    GAGTTAGTAGAAGCGAACGATGAAGTTGAAGTTCAATTAACTTTTGAAAAACCATTATCT
    CGCAAAGGCCGTAATGGTAAACCAAGCGGTTCTCGTAACAGAAATAGTAAGCGTGGTAAT
    CCTAAATTTGACAGTAAGAGTAAACGTTCAAAAGGATACTCAAGTAAGAAGAAAAGTACA
    AAAAAATTCGACCGTAAAGAGAAGAGCAGCGGTGGAAGCAGACCTATGAAAGGTCGCACA
    TTTGCTGACCATCAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1521

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS10845 [old locus tag: SA1885 ]
  • symbol: SA_RS10845
  • description: DEAD/DEAH box family ATP-dependent RNA helicase
  • length: 506
  • theoretical pI: 10.144
  • theoretical MW: 56942
  • GRAVY: -0.578854

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.4.13?  ExPASy
    RNA helicase ATP + H2O = ADP + phosphate
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 87)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 75.2)
    and 11 more
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 54.8)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 46)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 41.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 36.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair reverse gyrase (TIGR01054; EC 3.6.4.12,5.99.1.3; HMM-score: 31.3)
    CRISPR-associated helicase Cas3 (TIGR01587; HMM-score: 26.6)
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 21.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 19.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 18.4)
    CRISPR-associated helicase Cas3, subtype CYANO (TIGR03158; HMM-score: 12.9)
    Unknown function Enzymes of unknown specificity ATP-dependent helicase HrpB (TIGR01970; EC 3.6.4.-; HMM-score: 10.8)
  • TheSEED: see SA1885
  • PFAM:
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 169)
    and 7 more
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 112.7)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 25.9)
    CMS1; U3-containing 90S pre-ribosomal complex subunit (PF14617; HMM-score: 17)
    AAA_30; AAA domain (PF13604; HMM-score: 14.8)
    Flavi_DEAD; Flavivirus DEAD domain (PF07652; HMM-score: 13.4)
    ERCC3_RAD25_C; ERCC3/RAD25/XPB C-terminal helicase (PF16203; HMM-score: 12.2)
    no clan defined INTS5_N; Integrator complex subunit 5 N-terminus (PF14837; HMM-score: 10.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.021021
    • TAT(Tat/SPI): 0.001746
    • LIPO(Sec/SPII): 0.001025
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILGQAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVTVFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGFIDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEFYTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQAKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRAGKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSKESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSGSRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS11130(deoA)pyrimidine-nucleoside phosphorylase  [2] (data from MRSA252)
    SA_RS01365L-lactate dehydrogenase  [2] (data from MRSA252)
    SA_RS017105'-nucleotidase, lipoprotein e(P4) family  [2] (data from MRSA252)
    SA_RS0290550S ribosomal protein L11  [2] (data from MRSA252)
    SA_RS0291050S ribosomal protein L1  [2] (data from MRSA252)
    SA_RS0291550S ribosomal protein L10  [2] (data from MRSA252)
    SA_RS02930DNA-directed RNA polymerase subunit beta  [2] (data from MRSA252)
    SA_RS02955elongation factor G  [2] (data from MRSA252)
    SA_RS02960elongation factor Tu  [2] (data from MRSA252)
    SA_RS03155phosphate acetyltransferase  [2] (data from MRSA252)
    SA_RS03250zinc-dependent alcohol dehydrogenase  [2] (data from MRSA252)
    SA_RS04140aldehyde dehydrogenase  [2] (data from MRSA252)
    SA_RS05325ribonuclease J 1  [2] (data from MRSA252)
    SA_RS0614050S ribosomal protein L19  [2] (data from MRSA252)
    SA_RS06235elongation factor Ts  [2] (data from MRSA252)
    SA_RS06325ribonuclease J 2  [2] (data from MRSA252)
    SA_RS07385DNA-binding protein HU  [2] (data from MRSA252)
    SA_RS07605phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating)  [2] (data from MRSA252)
    SA_RS0829550S ribosomal protein L21  [2] (data from MRSA252)
    SA_RS08435trigger factor  [2] (data from MRSA252)
    SA_RS08600universal stress protein  [2] (data from MRSA252)
    SA_RS0867530S ribosomal protein S4  [2] (data from MRSA252)
    SA_RS1105050S ribosomal protein L31 type B  [2] (data from MRSA252)
    SA_RS11245glutamine--fructose-6-phosphate aminotransferase  [2] (data from MRSA252)
    SA_RS11430Asp23/Gls24 family envelope stress response protein  [2] (data from MRSA252)
    SA_RS1163050S ribosomal protein L17  [2] (data from MRSA252)
    SA_RS1164030S ribosomal protein S11  [2] (data from MRSA252)
    SA_RS1164530S ribosomal protein S13  [2] (data from MRSA252)
    SA_RS1167050S ribosomal protein L15  [2] (data from MRSA252)
    SA_RS1168030S ribosomal protein S5  [2] (data from MRSA252)
    SA_RS1169050S ribosomal protein L6  [2] (data from MRSA252)
    SA_RS1170550S ribosomal protein L5  [2] (data from MRSA252)
    SA_RS1171550S ribosomal protein L14  [2] (data from MRSA252)
    SA_RS1172030S ribosomal protein S17  [2] (data from MRSA252)
    SA_RS1173530S ribosomal protein S3  [2] (data from MRSA252)
    SA_RS1174050S ribosomal protein L22  [2] (data from MRSA252)
    SA_RS1175050S ribosomal protein L2  [2] (data from MRSA252)
    SA_RS1175550S ribosomal protein L23  [2] (data from MRSA252)
    SA_RS1176050S ribosomal protein L4  [2] (data from MRSA252)
    SA_RS1176550S ribosomal protein L3  [2] (data from MRSA252)
    SA_RS13735malate:quinone oxidoreductase  [2] (data from MRSA252)
    SA_RS13915ornithine carbamoyltransferase  [2] (data from MRSA252)
    SA_RS13920arginine deiminase  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 2.35 2.36 2.37 2.38 2.39 2.40 2.41 2.42 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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