PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS11560 [old locus tag: SA2008 ]
• pan locus tag^?: SAUPAN005625000
• symbol: SA_RS11560
• pan gene symbol^?: budB
• synonym:
• product: acetolactate synthase AlsS

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS11560 [old locus tag: SA2008 ]
• symbol: SA_RS11560
• product: acetolactate synthase AlsS
• replicon: chromosome
• strand: -
• coordinates: 2279807..2281471
• length: 1665
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2279807..2281471) NCBI
• BioCyc: SA_RS11560 BioCyc
• MicrobesOnline: see SA2008

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  
  ATGACTGATAAAAAGTACACTGCAGCCGATATGGTTATTGATACTTTGAAAAATAATGGG
  GTAGAATATGTTTTTGGTATTCCGGGTGCAAAGATAGACTATCTATTTAATGCTTTAATT
  GATGATGGTCCTGAACTTATTGTCACTCGTCATGAACAAAATGCTGCAATGATGGCACAA
  GGTATTGGAAGATTAACAGGTAAACCGGGTGTAGTACTTGTTACAAGTGGCCCTGGTGTA
  AGTAATTTAACGACTGGACTATTAACAGCTACATCTGAAGGGGATCCTGTATTAGCGTTA
  GGTGGCCAAGTGAAACGTAATGATTTATTACGATTAACGCATCAAAGTATTGATAATGCT
  GCGCTATTAAAATATTCATCAAAATACAGTGAAGAAGTACAAGATCCTGAATCATTATCA
  GAAGTTATGACAAATGCAATTCGAATTGCTACTTCAGGAAAAAATGGCGCAAGTTTTATT
  AGTATTCCGCAAGACGTTATTTCTTCACCAGTTGAATCTAAAGCTATATCACTTTGCCAA
  AAACCAAATTTAGGAGTACCGAGTGAACAAGATATTAATGATGTCATTGAAGCGATTAAA
  AATGCATCATTTCCTGTTTTATTAGCTGGTATGAGAAGTTCAAGTGCAGAAGAAACAAAT
  GCCATTCGCAAATTAGTTGAGCGCACGAATTTACCAGTTGTAGAAACATTCCAAGGTGCA
  GGTGTAATTAGTCGTGAATTAGAAAATCATTTCTTCGGTCGTGTGGGCTTATTCCGCAAT
  CAAGTTGGTGATGAATTATTACGTAAAAGTGATTTAGTTGTTACAATCGGTTATGATCCA
  ATTGAATACGAAGCTAGTAACTGGAATAAAGAATTAGAAACACAAATTATCAATATTGAC
  GAAGTTCAAGCTGAAATTACTAATTATATGCAACCGAAAAAAGAGTTGATTGGTAATATT
  GCTAAAACGATTGAAATGATTTCTGAAAAAGTGGATGAGCCATTTATAAATCAACAACAT
  TTAGACGAATTAGAACAATTAAGAACACATATTGATGAAGAAACTGGTATTAAAGCGACG
  CATGAAGAAGGAATTCTACATCCAGTGGAAATTATTGAATCTATGCAAAAGGTATTAACT
  GATGATACTACTGTAACAGTTGATGTTGGAAGTCACTATATTTGGATGGCACGTAATTTC
  AGAAGTTACAATCCAAGACATTTATTATTTAGCAATGGTATGCAAACGCTTGGTGTAGCA
  TTACCGTGGGCAATTTCAGCTGCACTTGTGCGCCCTAATACGCAAGTTGTGTCCGTTGCT
  GGCGATGGTGGCTTTTTATTTTCATCACAAGATTTAGAAACGGCCGTACGTAAAAATTTA
  AATATCATCCAGCTTATTTGGAATGATGGAAAATATAACATGGTTGAATTCCAAGAAGAA
  ATGAAATATAAACGTTCGTCAGGTGTAGACTTCGGTCCTGTAGATTTTGTAAAATATGCA
  GAATCATTTGGCGCGAAAGGTTTACGAGTTACTAATCAAGAAGAATTAGAAGCGGCAATT
  AAAGAGGGCTATGAAACAGATGGTCCAGTATTAATTGATATACCTGTAAATTACAAAGAT
  AATATCAAACTTTCAACAAATATGTTACCTGACGTATTTAACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1665
  

⊟Protein

⊟General

• locus tag: SA_RS11560 [old locus tag: SA2008 ]
• symbol: SA_RS11560
• description: acetolactate synthase AlsS
• length: 554
• theoretical pI: 4.51625
• theoretical MW: 61168.8
• GRAVY: -0.222383

⊟Function

• reaction:
  EC 2.2.1.6?  ExPASy
  Acetolactate synthase 2 pyruvate = 2-acetolactate + CO₂
• TIGRFAM:
  Metabolism Energy metabolism Fermentation acetolactate synthase, catabolic (TIGR02418; EC 2.2.1.6; HMM-score: 931.6)
  and 15 more
  Metabolism Amino acid biosynthesis Pyruvate family acetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 346.1)
  Metabolism Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 241.1)
  Cellular processes Cellular processes Detoxification oxalyl-CoA decarboxylase (TIGR03254; EC 4.1.1.8; HMM-score: 197.2)
  Metabolism Central intermediary metabolism Other sulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 187.4)
  glyoxylate carboligase (TIGR01504; EC 4.1.1.47; HMM-score: 169.4)
  Metabolism Energy metabolism Sugars 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 161.9)
  indolepyruvate/phenylpyruvate decarboxylase (TIGR03394; EC 4.1.1.43,4.1.1.74; HMM-score: 93.6)
  Metabolism Central intermediary metabolism Other indolepyruvate decarboxylase (TIGR03393; EC 4.1.1.74; HMM-score: 62.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (TIGR00173; EC 2.2.1.9; HMM-score: 57.6)
  phosphonopyruvate decarboxylase (TIGR03297; EC 4.1.1.82; HMM-score: 25.8)
  sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 25.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 21.2)
  Metabolism Energy metabolism Methanogenesis sulfopyruvate decarboxylase, alpha subunit (TIGR03845; EC 4.1.1.79; HMM-score: 21.2)
  2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family (TIGR02177; HMM-score: 19.3)
  Metabolism Energy metabolism Chemoautotrophy CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00315; EC 1.2.99.2; HMM-score: 17.2)
• TheSEED: see SA2008
• PFAM:
  THDP-binding (CL0254) TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 176)
  TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 150)
  and 4 more
  FAD_DHS (CL0085) TPP_enzyme_M; Thiamine pyrophosphate enzyme, central domain (PF00205; HMM-score: 88.8)
  P-loop_NTPase (CL0023) AAA_23; AAA domain (PF13476; HMM-score: 17.6)
  FAD_DHS (CL0085) CO_dh; CO dehydrogenase beta subunit/acetyl-CoA synthase epsilon subunit (PF02552; HMM-score: 14.6)
  Fusion_gly (CL0595) Baculo_F; Baculovirus F protein (PF12259; HMM-score: 10.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004002
  • TAT(Tat/SPI): 0.000185
  • LIPO(Sec/SPII): 0.000309
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446052294 NCBI
• RefSeq: WP_000130149 NCBI
• UniProt: see SA2008

⊟Protein sequence

• MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGPELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGGQVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFISIPQDVISSPVESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSAEETNAIRKLVERTNLPVVETFQGAGVISRELENHFFGRVGLFRNQVGDELLRKSDLVVTIGYDPIEYEASNWNKELETQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISEKVDEPFINQQHLDELEQLRTHIDEETGIKATHEEGILHPVEIIESMQKVLTDDTTVTVDVGSHYIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQDLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGLRVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications