From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS12070 [old locus tag: SA2100 ]
  • pan locus tag?: SAUPAN005782000
  • symbol: SA_RS12070
  • pan gene symbol?: —
  • synonym:
  • product: N-acetylmuramoyl-L-alanine amidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS12070 [old locus tag: SA2100 ]
  • symbol: SA_RS12070
  • product: N-acetylmuramoyl-L-alanine amidase
  • replicon: chromosome
  • strand: -
  • coordinates: 2360658..2361296
  • length: 639
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2360658..2361296) NCBI
  • BioCyc: SA_RS12070 BioCyc
  • MicrobesOnline: see SA2100

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCAACAAGGGAAAGGTATTGTACAGACAAAAGAAGAGGATGGTAAATTTGTTGAAGCA
    AATAATAATGAAATTGCTAAAGCAATGACTATTTCACATAAAGACAATGATATGAAGTAT
    ATGGATATCACAGAAAAAGTGCCAATGTCGGAATCTGAAGTTAACCAATTGCTAAAAGGT
    AAGGGGATTTTAGAAAATCGAGGGAAAGTTTTTCTAGAAGCTCAAGAAAAATATGAGGTT
    AATGTCATTTATCTTGTTAGCCATGCATTAGTAGAAACAGGTAACGGCAAATCAGAATTA
    GCAAAAGGCATTAAAGATGGGAAAAAACGCTATTACAACTTTTTTGGTATAGGAGCATTC
    GATAGTAGTGCTGTTCGTAGTGGGAAAAGTTATGCTGAAAAGGAACAATGGACATCACCA
    GATAAGGCGATTATTGGTGGTGCAAAGTTCATTCGTAATGAATATTTTGAAAACAATCAA
    CTGAATTTATATCAAATGCGATGGAATCCAGAAAATCCTGCGCAACATCAATATGCGAGT
    GACATTCGATGGGCAGATAAAATTGCCAAATTAATGGATAAATCCTATAAGCAGTTTGGT
    ATAAAGAAAGATGATATTAGACAAACATATTATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    639

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS12070 [old locus tag: SA2100 ]
  • symbol: SA_RS12070
  • description: N-acetylmuramoyl-L-alanine amidase
  • length: 212
  • theoretical pI: 9.4162
  • theoretical MW: 24389.4
  • GRAVY: -0.851887

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA-3-methyladenine glycosylase I (TIGR00624; EC 3.2.2.20; HMM-score: 12)
  • TheSEED: see SA2100
  • PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 64.3)
    and 1 more
    no clan defined Dcc1; Sister chromatid cohesion protein Dcc1 (PF09724; HMM-score: 17)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.1
    • Cytoplasmic Membrane Score: 0.14
    • Cellwall Score: 0.15
    • Extracellular Score: 9.6
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007516
    • TAT(Tat/SPI): 0.000494
    • LIPO(Sec/SPII): 0.001589
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQLLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNFFGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPENPAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA_RS12070&oldid=83240"