PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS12975 [old locus tag: SA2260 ]
• pan locus tag^?: SAUPAN006035000
• symbol: SA_RS12975
• pan gene symbol^?: —
• synonym:
• product: short-chain dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS12975 [old locus tag: SA2260 ]
• symbol: SA_RS12975
• product: short-chain dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 2537697..2538515
• length: 819
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2537697..2538515) NCBI
• BioCyc: SA_RS12975 BioCyc
• MicrobesOnline: see SA2260

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAACGTTTGGAAAATAAAGTTGCAGTCGTAACAGGAGCAAGTACAGGTATCGGACAA
  GCTTCTGCAATCGCTTTAGCTCAAGAAGGTGCGTATGTATTGGCGGTAGACATAGCTGAA
  GCGGTATCGGAGACTGTCGATAAAATTAAAAGTAACGGTGACAATGCGAAGGCGTATAAT
  GTGGATATTTCAGATGAACAACAAGTGGTAGACTTTGTGTCTGACATAAAGGAACAGTTT
  GGAAGAATCGATGTGTTGTTTAATAATGCCGGTGTGGATAATGCGGCTGGTAGAATTCAT
  GAGTATCCAATAGATGTGTATGACAAGATTATGAATGTAGATATGCGTGGGACAATTTTA
  ATGACGAAAATGATGTTACCTTTAATGATGAATCAAGGTGGCTCTATTGTTAATACGTCT
  TCATTTTCAGGACAGGCAGAAGATTTGTATCGCTCAGGATATAATGCTGCGAAAGGCGCA
  GTGATTAATTTTACAAAATCAATCGCAATTGAGTATGGTCGTGATGGCATTCGATCCAAT
  GCAATTGCACCAGGTACAATTGAAACCCCGTTAGTAGATAAACTGACAGGTACGAGTGAG
  GATGATGCAGGTAAAACATTTAGAGAAAATCAAAAATGGATGACTCCGCTGGGACGTTTA
  GGTAAACCAGAAGAAGTTGCTAAATTAGTAGTCTTCTTAGCATCTGACGACAGTTCATTC
  ATAACTGGAGAGACGATTCGAATTGATGGTGGTGTGATGGCTTACACATGGCCTGGCGAG
  ATGTTAAGTGATGATTCGTGGAAGCGGACATTGGAATAA
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  819
  

⊟Protein

⊟General

• locus tag: SA_RS12975 [old locus tag: SA2260 ]
• symbol: SA_RS12975
• description: short-chain dehydrogenase
• length: 272
• theoretical pI: 4.40492
• theoretical MW: 29453.1
• GRAVY: -0.191912

⊟Function

• TIGRFAM:
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 194.9)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 191.8)
  3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 183.7)
  2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 170)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 165)
  Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 164.1)
  2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 162.8)
  acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 156.3)
  and 8 more
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 128.3)
  rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 113.2)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 111.4)
  cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 87.9)
  pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 70.2)
  sepiapterin reductase (TIGR01500; EC 1.1.1.153; HMM-score: 39.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll light-dependent protochlorophyllide reductase (TIGR01289; EC 1.3.1.33; HMM-score: 33.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 16.9)
• TheSEED: see SA2260
• PFAM:
  NADP_Rossmann (CL0063) adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 235.2)
  and 6 more
  adh_short; short chain dehydrogenase (PF00106; HMM-score: 176.7)
  KR; KR domain (PF08659; HMM-score: 45)
  Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 18.9)
  Eno-Rase_NADH_b; NAD(P)H binding domain of trans-2-enoyl-CoA reductase (PF12242; HMM-score: 16)
  3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 14.2)
  PH (CL0266) OCRL_clath_bd; Inositol polyphosphate 5-phosphatase clathrin binding domain (PF16726; HMM-score: 14)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.5
  • Cellwall Score: 4.07
  • Extracellular Score: 5.43
  • Internal Helices: 0
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 27 aa
  • SP(Sec/SPI): 0.698318
  • TAT(Tat/SPI): 0.035546
  • LIPO(Sec/SPII): 0.00796
  • Cleavage Site: CS pos: 27-28. ALA-QE. Pr: 0.4279
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446747692 NCBI
• RefSeq: WP_000824948 NCBI
• UniProt: see SA2260

⊟Protein sequence

• MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSETVDKIKSNGDNAKAYNVDISDEQQVVDFVSDIKEQFGRIDVLFNNAGVDNAAGRIHEYPIDVYDKIMNVDMRGTILMTKMMLPLMMNQGGSIVNTSSFSGQAEDLYRSGYNAAKGAVINFTKSIAIEYGRDGIRSNAIAPGTIETPLVDKLTGTSEDDAGKTFRENQKWMTPLGRLGKPEEVAKLVVFLASDDSSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY see SA2260

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications