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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS13995 [old locus tag: SA2442 ]
  • pan locus tag?: SAUPAN006382000
  • symbol: SA_RS13995
  • pan gene symbol?: secA2
  • synonym:
  • product: accessory Sec system translocase SecA2

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS13995 [old locus tag: SA2442 ]
  • symbol: SA_RS13995
  • product: accessory Sec system translocase SecA2
  • replicon: chromosome
  • strand: -
  • coordinates: 2747473..2749863
  • length: 2391
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2747473..2749863) NCBI
  • BioCyc: SA_RS13995 BioCyc
  • MicrobesOnline: see SA2442

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
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    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
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    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAAACATAAACTAGATGTTACGATAAACGAATTAAGACTAAAGTCGATTAGAAAAATA
    GTGAAACGCATCAATACATGGAGCGATGAAGTTAAATCATATTCTGATGATGCGTTAAAA
    CAAAAGACAATAGAATTTAAAGAACGTTTAGCATCAGGAGTTGATACACTAGATACATTG
    TTACCTGAAGCTTATGCAGTGGCACGTGAAGCGAGCTGGAGAGTATTAGGTATGTATCCT
    AAAGAAGTTCAGTTAATCGGAGCGATTGTATTGCATGAAGGTAACATTGCGGAGATGCAA
    ACAGGCGAAGGTAAAACATTAACGGCAACGATGCCTTTATATTTAAATGCTCTTTCGGGA
    AAAGGAACATATTTAATTACAACGAATGATTATTTAGCGAAGCGAGATTTTGAAGAGATG
    CAACCATTATATGAATGGTTAGGTTTAACTGCTTCATTAGGTTTTGTTGATATTGTGGAT
    TATGAGTATCAAAAAGGGGAAAAACGTAATATATATGAACATGATATTATATATACAACT
    AATGGACGTCTCGGTTTTGATTATTTAATTGATAATTTGGCTGATAGTGCGGAAGGGAAA
    TTTTTACCACAATTAAATTACGGTATTATTGATGAAGTGGATTCAATCATTTTAGATGCT
    GCTCAAACACCATTAGTTATTTCGGGTGCACCAAGATTACAATCAAATCTATTTCATATT
    GTGAAAGAGTTTGTAGATACATTGATTGAAGACGTGCATTTTAAAATGAAGAAGACCAAA
    AAAGAAATATGGCTGTTAAATCAAGGTATTGAAGCGGCACAATCATACTTTAATGTTGAA
    GATTTATATAGCGAACAAGCGATGGTCCTAGTGCGTAATATTAATTTAGCACTGCGCGCA
    CAATATTTGTTTGAATCTAATGTCGATTACTTTGTATATAATGGTGATATTGTTTTAATT
    GACCGTATTACAGGTCGTATGTTACCGGGAACTAAGTTGCAAGCTGGACTTCACCAAGCT
    ATTGAAGCGAAAGAAGGTATGGAGGTTTCAACAGATAAAAGTGTTATGGCAACAATTACC
    TTCCAGAATTTATTTAAACTTTTTGAATCATTTTCAGGTATGACAGCTACAGGAAAATTA
    GGCGAATCAGAGTTCTTTGATTTGTATTCAAAAATAGTCGTACAAGCACCAACTGATAAA
    GCGATTCAACGTATCGATGAACCAGATAAAGTGTTTCGTTCAGTTGATGAGAAAAACATC
    GCGATGATTCATGATATAGTTGAACTTCATGAAACGGGGCGACCGGTTTTACTCATAACG
    AGAACTGCTGAAGCGGCTGAATACTTTTCGAAAGTATTATTCCAAATGGATATTCCTAAT
    AATTTACTCATTGCGCAAAATGTTGCAAAAGAAGCGCAGATGATAGCTGAAGCAGGGCAA
    ATTGGTTCCATGACTGTTGCGACTAGTATGGCAGGTCGAGGCACAGATATTAAACTTGGT
    GAAGGTGTCGAAGCATTAGGTGGATTAGCTGTTATTATTCATGAACATATGGAAAATAGC
    CGTGTAGACAGGCAATTACGTGGTCGTTCTGGTAGACAAGGGGATCCGGGATCATCTTGT
    ATATATATTTCACTAGATGATTATTTAGTTAAGCGATGGAGCGATAGTAATTTAGCGGAA
    AATAATCAATTATATTCATTAGATGCACAACGATTATCGCAAAGTAATTTGTTTAATCGC
    AAAGTTAAGCAAATTGTAGTTAAAGCGCAGCGTATCTCGGAAGAACAAGGGGTTAAAGCT
    CGTGAAATGGCTAATGAATTTGAAAAAAGCATTAGTATACAGCGAGATCTTGTATACGAG
    GAACGCAATCGAGTTTTAGAAATAGATGATGCTGAGAATCGAGATTTTAAAGCGTTAGCT
    AAAGATGTATTTGAAATGTTTGTAAATGAGGAAAAGGTGCTAACAAAATCGCGTGTTGTC
    GAGTATATTTATCAAAATTTAAGTTTCCAATTTAATAAAGATGTGGCTTGTGTTAATTTT
    AAAGATAAGCAAGCAGTAGTGACATTTTTATTAGAGCAATTTGAAAAGCAATTAGCTTTG
    AATCGTAAAAACATGCAAAGTGCATATTATTATAATATTTTCGTTCAAAAAGTCTTTTTG
    AAAGCAATTGATTCATGTTGGTTAGAACAAGTCGACTATTTACAACAATTAAAAGCAAGC
    GTTAATCAACGACAAAATGGGCAACGTAACGCTATTTTTGAATACCATAGAGTTGCATTA
    GATTCATTTGAAGTTATGACACGAAATATCAAAAAAAGAATGGTGAAAAATATTTGTCAA
    AGCATGATTACATTTGATAAAGAGGGTATGCCAGTTATACATTTTCCATAA
    60
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    1920
    1980
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    2160
    2220
    2280
    2340
    2391

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS13995 [old locus tag: SA2442 ]
  • symbol: SA_RS13995
  • description: accessory Sec system translocase SecA2
  • length: 796
  • theoretical pI: 5.37006
  • theoretical MW: 90929.4
  • GRAVY: -0.269975

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system translocase SecA2 (TIGR03714; HMM-score: 1238.3)
    and 3 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking preprotein translocase, SecA subunit (TIGR00963; HMM-score: 685.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking accessory Sec system translocase SecA2 (TIGR04397; HMM-score: 666.3)
    accessory Sec system translocase SecA2 (TIGR04221; HMM-score: 481.7)
  • TheSEED: see SA2442
  • PFAM:
    P-loop_NTPase (CL0023) SecA_DEAD; SecA DEAD-like domain (PF07517; HMM-score: 340.6)
    and 4 more
    no clan defined SecA_PP_bind; SecA preprotein cross-linking domain (PF01043; HMM-score: 91.1)
    SecA_SW; SecA Wing and Scaffold domain (PF07516; HMM-score: 87.5)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 27.6)
    no clan defined Mrx7; MIOREX complex component 7 (PF10906; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007036
    • TAT(Tat/SPI): 0.000872
    • LIPO(Sec/SPII): 0.00129
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTIEFKERLASGVDTLDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTGEGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVDYEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIILDAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSYFNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQAGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKIVVQAPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFSKVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGGLAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYSLDAQRLSQSNLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNRVLEIDDAENRDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKDKQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKASVNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SA_RS00310aminoglycoside O-nucleotidyltransferase ANT(4')-Ia  [2] (data from MRSA252)
    SA_RS01275formate acetyltransferase  [2] (data from MRSA252)
    SA_RS0202530S ribosomal protein S18  [2] (data from MRSA252)
    SA_RS02955elongation factor G  [2] (data from MRSA252)
    SA_RS02960elongation factor Tu  [2] (data from MRSA252)
    SA_RS04575NADH dehydrogenase  [2] (data from MRSA252)
    SA_RS05350pyruvate dehydrogenase E1 component subunit alpha  [2] (data from MRSA252)
    SA_RS0622530S ribosomal protein S2  [2] (data from MRSA252)
    SA_RS06235elongation factor Ts  [2] (data from MRSA252)
    SA_RS07060dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex  [2] (data from MRSA252)
    SA_RS08545isocitrate dehydrogenase (NADP(+))  [2] (data from MRSA252)
    SA_RS08560pyruvate kinase  [2] (data from MRSA252)
    SA_RS08625universal stress protein UspA  [2] (data from MRSA252)
    SA_RS09850aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B  [2] (data from MRSA252)
    SA_RS11010uracil phosphoribosyltransferase  [2] (data from MRSA252)
    SA_RS1105050S ribosomal protein L31 type B  [2] (data from MRSA252)
    SA_RS11430Asp23/Gls24 family envelope stress response protein  [2] (data from MRSA252)
    SA_RS1160030S ribosomal protein S9  [2] (data from MRSA252)
    SA_RS1173530S ribosomal protein S3  [2] (data from MRSA252)
    SA_RS1175550S ribosomal protein L23  [2] (data from MRSA252)
    SA_RS1176050S ribosomal protein L4  [2] (data from MRSA252)
    SA_RS13735malate:quinone oxidoreductase  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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