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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1925 [new locus tag: NWMN_RS11110 ]
  • pan locus tag?: SAUPAN005188000
  • symbol: int
  • pan gene symbol?: int3
  • synonym:
  • product: phage integrase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1925 [new locus tag: NWMN_RS11110 ]
  • symbol: int
  • product: phage integrase
  • replicon: chromosome
  • strand: +
  • coordinates: 2131160..2132221
  • length: 1062
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    TTGCCAATTTTGAGGAGGGAACGCATGAAAACACGTTGTTACGATGGTAAAAAATGGCAA
    TATGAATTTAAGCATGAAGGAAAAAGATACCGTAAGAAAGGTTTTAGAACAAAGCGTGAA
    GCTAATTCTGCTGGACTAGACAAGTTAAATGAGTTAAGAAGTGGTTTTAATATAGATAAC
    TATATAACTCTTGAAGAATACTTCGAAAATTGGATTAAAACATATAAACAACCTGTTGTT
    AAAGAAAATACCTACCGTCATTATAGAAATGCATTACAACATATACAAAAACATAAAATA
    GGTAAAATGGAGTTATCAAAGATAAATAGACAAGTTTATCAGAAATTCATAAACGACTAT
    TCAAAAGAACACGCAAAAGAAACTATAAGAAAAACAAACGGTGCTATTCGGTCAGCTTTA
    GATGACGCATTATATGATGGACTTATTTTTAAAAACCCCGCTTATAAAGTTAATTATAAA
    GCCGGAAAACCTACGAAGTCAGAACAAGAAAAATTCATCTCGGTAACTGAATATGAAATA
    CTAAAAGATCACGTCAGAAAGAAGAGAACTCGTTCATCATTAGCGCTATTCATAATGATT
    TGTACGGGTTGTCGTGTCAGTGGTGCAAGAAATATAAAGATTGAGCATATCAACCAAGTG
    AAAAACACTATATTTATTGACGAGCGAAAAACCAATACTTCCCCTAGATATATCAGTATC
    GCTAAATCTGATATGAAACACATTATGGACGTCATAAGTACATTTGCAATTAGCTATGAT
    GGTTACATTTTCAAAGAAGGCGGATCTATAATTAACCTTCATGCTATCAATAATGCTTTG
    AAATCAGCCTGTAGAGTCAATAATATACCAATTATTACATCGCACGCATTAAGACACACT
    CATTGTTCTTATTTACTAGCAAAAGGTGTATCTATACATTACATTTCTAAAAGATTAGGT
    CATAAAAATATAGCAATAACTACATCTGTGTATTCTCATTTGTTAGAAGAAAAATTTAAT
    GAAGAGGACAAAAAAACAACTAAAATTTTAGAAAGTATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1062

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1925 [new locus tag: NWMN_RS11110 ]
  • symbol: Int
  • description: phage integrase
  • length: 353
  • theoretical pI: 10.3453
  • theoretical MW: 41340.4
  • GRAVY: -0.694901

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 71.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 70.4)
    and 2 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 44)
    Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 44)
  • TheSEED: data available for N315, NCTC8325, USA300_FPR3757
  • PFAM:
    DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 77.9)
    and 8 more
    l-integrase_N (CL0469) Phage_int_SAM_3; Phage integrase, N-terminal SAM-like domain (PF14659; HMM-score: 40.3)
    Phage_int_SAM_5; Phage integrase SAM-like domain (PF13102; HMM-score: 36.2)
    MBD-like (CL0081) Arm-DNA-bind_4; Arm DNA-binding domain (PF14657; HMM-score: 16.7)
    l-integrase_N (CL0469) Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 16.1)
    no clan defined DUF1722; Protein of unknown function (DUF1722) (PF08349; HMM-score: 14)
    NblA; Phycobilisome degradation protein nblA (PF04485; HMM-score: 13)
    MACRO (CL0223) Macro; Macro domain (PF01661; HMM-score: 12.9)
    AEP (CL0243) Replicase; Replicase family (PF03090; HMM-score: 12)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007371
    • TAT(Tat/SPI): 0.00365
    • LIPO(Sec/SPII): 0.003029
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MPILRRERMKTRCYDGKKWQYEFKHEGKRYRKKGFRTKREANSAGLDKLNELRSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINRQVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSEQEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFIDERKTNTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEGGSIINLHAINNALKSACRVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEEDKKTTKILESM

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]