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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0275 [new locus tag: SAUSA300_RS01475 ]
  • pan locus tag?: SAUPAN001171000
  • symbol: SAUSA300_0275
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0275 [new locus tag: SAUSA300_RS01475 ]
  • symbol: SAUSA300_0275
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 327963..328616
  • length: 654
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGAATTGAAAGTCGATGATTTTGTAAAGAATATAAAAAGACCATACTTGACTGTATTG
    GGAGTATTTGTAGTTGCAGTTTCATTATTTTTTGACGCGATTATGTTTTTCTACGCTAAA
    CTATATGATAAATTGCCTATGTATTTACTGGTGTTTATGGCCTTTACAGCTGTAATTTTG
    ATTATGATGTACATACAAGAGAAAAATGAAAATTACAAAGTTGAAAAAAGATATGTGGTT
    AGATATCTCACACTTAACGTTATTGTGGGATATACTTTGCCATTGCTTTTTGTATCTATT
    TACGTTTTTGGTGTAGTCGGTTTTGGATTTGATGTTTTCAATTATTGTCTAGGTATTATC
    TTGATGTTATTTATTTCTTGGTTAGGTTTATTTTTATTTTATAAAAACGAATTTGATAGT
    GAAAATCCTAATCCTGCAGTTAATGCCATAGCAATTATTATAAAATTATTTGCTTTTGGA
    GGAATATTTTATATAAGTCTAATAGTTCCTGTAACACAGCAAGAAGAGATTTTTATAGGG
    TTAAGTATATTCATCAATATAATTGTTGATGCTCTTCTTGTTAGGTCATATTTTAATTAC
    GCGTTATATAAGAGTATTAAGAAAGATATCGAAAATGAAGGTAAGACAATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    654

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_0275 [new locus tag: SAUSA300_RS01475 ]
  • symbol: SAUSA300_0275
  • description: hypothetical protein
  • length: 217
  • theoretical pI: 6.71765
  • theoretical MW: 25183
  • GRAVY: 0.82765

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Photosynthesis cytochrome b559, alpha subunit (TIGR01332; HMM-score: 11.9)
    and 1 more
    exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase (TIGR03025; HMM-score: 3.2)
  • TheSEED  :
    • FIG01108255: hypothetical protein
  • PFAM:
    no clan defined DUF5079; Domain of unknown function (DUF5079) (PF16882; HMM-score: 243.2)
    and 8 more
    DUF2157; Predicted membrane protein (DUF2157) (PF09925; HMM-score: 17.6)
    GPCR_A (CL0192) Ceramidase; Ceramidase (PF05875; HMM-score: 15.5)
    no clan defined DUF3624; Protein of unknown function (DUF3624) (PF12292; HMM-score: 12.8)
    DUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 11.4)
    P12; Virus attachment protein p12 family (PF12669; HMM-score: 10)
    DUF3180; Protein of unknown function (DUF3180) (PF11377; HMM-score: 8.7)
    DUF3397; Protein of unknown function (DUF3397) (PF11877; HMM-score: 8.2)
    Jiraiya; Jiraiya (PF15038; HMM-score: 5.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000557
    • TAT(Tat/SPI): 0.000116
    • LIPO(Sec/SPII): 0.008764
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MELKVDDFVKNIKRPYLTVLGVFVVAVSLFFDAIMFFYAKLYDKLPMYLLVFMAFTAVILIMMYIQEKNENYKVEKRYVVRYLTLNVIVGYTLPLLFVSIYVFGVVGFGFDVFNYCLGIILMLFISWLGLFLFYKNEFDSENPNPAVNAIAIIIKLFAFGGIFYISLIVPVTQQEEIFIGLSIFINIIVDALLVRSYFNYALYKSIKKDIENEGKTM

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]