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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_00341
- pan locus tag?: SAUPAN001903000
- symbol: SAOUHSC_00341
- pan gene symbol?: metI
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_00341
- symbol: SAOUHSC_00341
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 355117..356220
- length: 1104
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3921815 NCBI
- RefSeq: YP_498931 NCBI
- BioCyc: G1I0R-317 BioCyc
- MicrobesOnline: 1288825 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1081ATGAAGGATACACAGTTAGCCCAAATCACATTAACCGATGATTCAACCGGTGCTATAGCG
AATCCAATCCATTTATCTACTGCCTACAAGCATCCAAAACTAGGACAATCGACAGGTTTT
GATTATACACGTACTAAAAATCCTACACGCTCAACATTTGAAACCTGTTTTGCCAAACTT
GAGCATGGTATTGCATCATTCGCTACATCAAGTGGAATGTCAGCCATTCAATTAATATGT
AATCTATTTAAACCTCATGATGAAATTTTAGTTTCATTCGATTTATATGGTGGCACATTT
AGATTATTTGAATTTTACGAGCAACAATACGATATCAAATTTAAGTACGTTGATTTTACA
GATTATGAACAAGTTGAAAAAGAAATCACTGATAAAACAGTTGCATTATTCATTGAACCA
ATATCTAACCCACAAATGATTGCTATTGATGTAAAGCCATACTATCAACTTTGTAAAGCT
AAAGGCTTATTGTCAATTATCGACAATACTTTTTTAACACCTTATCTTTCAACACCACTA
GCAGAAGGTGCTGATATAGTCTTACATTCAGCCACGAAATATATTGGCGGACATAACGAT
GTACTAGCAGGTGTCGTAACCGTCAAAGATGAATCACTCGCGCAACAGTTGTTTGATTTT
CACAACATGACTGGCGCAACACTTTCACCAATAGATAGTTATTTGTTGTTACGTGGACTT
AAAACTTTGCATTTACGCATTGAGCGTGCGCAATCAAACGCTAGAAAACTTGCTAAAAAA
TGTCAGTCACTTCAAGCAATTGACGAAGTACTATATAGCGGGCAAACTGGCATGCTTAGT
TTAAGACTTAACAAGGCCTATAGCGTCGCTAAATTATTAGAAAATTTAGACATTTGCATT
TTTGCAGAAAGTTTAGGAGGTACTGAAACATTAGTGACCTTCCCTTACACCCAAACACAT
GTTGATATGCCAGATGCTGAAAAAGATAAACGTGGCATTGATGAGTATTTAATCCGCTTA
TCACTAGGTGTTGAAAATTATGAAGACATCGAACGCGATATCATCCAAGCATTAGATAAA
GCTCAGATTGGAGAGATTGTATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_00341
- symbol: SAOUHSC_00341
- description: hypothetical protein
- length: 367
- theoretical pI: 5.01375
- theoretical MW: 41071.6
- GRAVY: -0.134332
⊟Function[edit | edit source]
- ⊞TIGRFAM: Amino acid biosynthesis Aspartate family O-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 312.8)Energy metabolism Amino acids and amines methionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 280.4)cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 277.7)and 8 more
- ⊞TheSEED :
- Cystathionine gamma-synthase (EC 2.5.1.48)
Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis Cystathionine gamma-synthase (EC 2.5.1.48)and 1 more - ⊞PFAM: PLP_aminotran (CL0061) Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 371.1)and 2 more
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: pyridoxal 5'-phosphate
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTRTKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRLFEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKPYYQLCKAKGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLFDFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQTGMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGIDEYLIRLSLGVENYEDIERDIIQALDKAQIGEIV
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- ⊟interaction partners:
SAOUHSC_01683 (dnaK) molecular chaperone DnaK [1] (data from MRSA252) SAOUHSC_00799 (eno) phosphopyruvate hydratase [1] (data from MRSA252) SAOUHSC_02496 (rplF) 50S ribosomal protein L6 [1] (data from MRSA252) SAOUHSC_00521 (rplL) 50S ribosomal protein L7/L12 [1] (data from MRSA252) SAOUHSC_01784 (rplT) 50S ribosomal protein L20 [1] (data from MRSA252) SAOUHSC_01757 (rplU) 50S ribosomal protein L21 [1] (data from MRSA252) SAOUHSC_02477 (rpsI) 30S ribosomal protein S9 [1] (data from MRSA252) SAOUHSC_01234 (tsf) elongation factor Ts [1] (data from MRSA252) SAOUHSC_00187 formate acetyltransferase [1] (data from MRSA252) SAOUHSC_00530 elongation factor Tu [1] (data from MRSA252) SAOUHSC_01040 pyruvate dehydrogenase complex, E1 component subunit alpha [1] (data from MRSA252) SAOUHSC_01041 pyruvate dehydrogenase complex, E1 component subunit beta [1] (data from MRSA252) SAOUHSC_01042 branched-chain alpha-keto acid dehydrogenase subunit E2 [1] (data from MRSA252) SAOUHSC_01806 pyruvate kinase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_00338 < SAOUHSC_00339 < SAOUHSC_00340 < SAOUHSC_00341predicted SigB promoter [2] : SAOUHSC_00337 < SAOUHSC_00338 < SAOUHSC_00339 < SAOUHSC_00340 < SAOUHSC_00341
⊟Regulation[edit | edit source]
- ⊞regulator: T-box(Met) (transcription antitermination) regulon
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [2] ⊟Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Jump up to: 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p) - ↑ Jump up to: 2.0 2.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)