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NCBI: 06-JUL-2013

⊟Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0105 [new locus tag: NWMN_RS00585 ]
  • pan locus tag?: SAUPAN000989000
  • symbol: capK
  • pan gene symbol?: capK
  • synonym:
  • product: capsular polysaccharide biosynthesis protein CapK

⊟Genome View[edit source | edit]

⊟Gene[edit source | edit]

⊟General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0105 [new locus tag: NWMN_RS00585 ]
  • symbol: capK
  • product: capsular polysaccharide biosynthesis protein CapK
  • replicon: chromosome
  • strand: +
  • coordinates: 129654..130838
  • length: 1185
  • essential: unknown other strains

⊟Accession numbers[edit source | edit]

⊟Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

⊟DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGATAGCGTAAAGACAATAATTGGTACGTTGCTTATAGCTTTAGGATTACAATTTTTA
    GCTTATCCAATTATTAATCAACGAGTAGGTAATGAAGCGTTCGGTTCTATTTTAACGATT
    TATACAATAATAACAATCACGAGTGTTGTATTAGGCAATACGCTTAACAATATACGATTG
    ATTAATATGAATCTATACAAATCCAATCATTACTACTGGAAATTTGCATCGATACTTTTA
    ATCTCAATTCTGATTGAGAGTATAGCTTTAATTATTGTATTTCTTTACTTTTTTAATTTG
    AACATCATCGATATTATCTTTTTAATTCTACTTAATATTTTAATGTGTTTAAGGATTTAT
    CTGAATGTATTTTTTAGGATGACTTTAAAATATAATCAGATTTTGTATATTGCTCTTATT
    CAATTTTTAGGTTTGCTGATAGGACTATTTCTATATTATTTAACCCAAAACTGGATTGTT
    TGTTTTATTACCAGTGAATTGTTTGCAACGATATATACATTGGTTAAATTACGGGGATTA
    ACTATAGGCGAGTATCAAAGTGAAGATAATAATGTGGTAAAAGATTATGTGATGCTACTG
    AGTACAAATAGCCTTAATAATTTGAATCTCTATTTAGATAGATTAATCTTATTACCAATT
    ATAGGTGGAACAGCTGTAACTATATCATTTCTTTCAACATTTATTGGGAAAATGTTAGCT
    ACATTTCTATATCCGATTAATAATGTAGTACTTTCATATATTTCTGTAAATGAAAGTGAC
    AATATAAAGAAGCAATATTTGAAAACTAATCTAATTGCTATAGCTGCCCTATGTTTAGTC
    ATGATTATATGTTATCCAATTACAATAATTATTGTCTCTTTACTGTATAACATTGATTCA
    AGTTTATATTCGAAGTTTATTATTTTAGGTAATATAGGTGTTTTATTCAATGCAGTGAGT
    ATTATGATCCAAACTTTAAATACAAAACACGCATCAATAACATTACAAGCGAATTATATG
    ACGCTTCACACGATTACATTTATATTCATAACTATTTTAATGACAATTGCGTTTGGTCTA
    AATGGATTCTTTTGGACAACGCTGTTCAGCAACATTATTAAGTATGTGATTTTAAATATT
    ATAGGTTTAAAGTCTAAATTCATTAATAAAAAGGACGTCGATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1185

⊟Protein[edit source | edit]

⊟General[edit source | edit]

  • locus tag: NWMN_0105 [new locus tag: NWMN_RS00585 ]
  • symbol: CapK
  • description: capsular polysaccharide biosynthesis protein CapK
  • length: 394
  • theoretical pI: 9.30526
  • theoretical MW: 44854.6
  • GRAVY: 0.957107

⊟Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Cell Wall and CapsuleCapsular and extracellular polysacchridesSerotype determining Capsular polysaccharide biosynthesis in Staphylococcus Capsular polysaccharide synthesis enzyme Cap5K 
  • PFAM:

⊟Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

⊟Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.143
    • Ymax_pos: 22
    • Cmax: 0.142
    • Cmax_pos: 36
    • Smax: 0.29
    • Smax_pos: 19
    • Smean: 0.197
    • D: 0.164
  • predicted transmembrane helices (TMHMM): 10

⊟Accession numbers[edit source | edit]

⊟Protein sequence[edit source | edit]

  • MDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSILTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFLYFFNLNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYYLTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLYLDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKTNLIAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLNTKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKSKFINKKDVD

⊟Experimental data[edit source | edit]

  • experimentally validated: no data available

⊟Expression & Regulation[edit source | edit]

⊟Operon[edit source | edit]

⊟Regulation[edit source | edit]

  • sigma factor: SigB [1] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator: CodY
    CodY (TF) important in Amino acid metabolismRegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern[edit source | edit]

⊟Protein synthesis (provided by Aureolib)[edit source | edit]

⊟Protein stability[edit source | edit]

  • half-life: no data available

⊟Biological Material[edit source | edit]

⊟Mutants[edit source | edit]

⊟Expression vector[edit source | edit]

⊟lacZ fusion[edit source | edit]

⊟GFP fusion[edit source | edit]

⊟two-hybrid system[edit source | edit]

⊟FLAG-tag construct[edit source | edit]

⊟Antibody[edit source | edit]

⊟Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

⊟Literature[edit source | edit]

⊟References[edit source | edit]

  1. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J. Bacteriol.: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit source | edit]

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