NCBI: 06-JUL-2013
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus Newman
- locus tag: NWMN_0105 [new locus tag: NWMN_RS00585 ]
- pan locus tag?: SAUPAN000989000
- symbol: capK
- pan gene symbol?: capK
- synonym:
- product: capsular polysaccharide biosynthesis protein CapK
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: NWMN_0105 [new locus tag: NWMN_RS00585 ]
- symbol: capK
- product: capsular polysaccharide biosynthesis protein CapK
- replicon: chromosome
- strand: +
- coordinates: 129654..130838
- length: 1185
- essential: unknown other strains
⊟Accession numbers[edit source | edit]
- Gene ID: 5331804 NCBI
- RefSeq: YP_001331140 NCBI
- BioCyc:
- MicrobesOnline: 3705636 MicrobesOnline
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
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1141ATGGATAGCGTAAAGACAATAATTGGTACGTTGCTTATAGCTTTAGGATTACAATTTTTA
GCTTATCCAATTATTAATCAACGAGTAGGTAATGAAGCGTTCGGTTCTATTTTAACGATT
TATACAATAATAACAATCACGAGTGTTGTATTAGGCAATACGCTTAACAATATACGATTG
ATTAATATGAATCTATACAAATCCAATCATTACTACTGGAAATTTGCATCGATACTTTTA
ATCTCAATTCTGATTGAGAGTATAGCTTTAATTATTGTATTTCTTTACTTTTTTAATTTG
AACATCATCGATATTATCTTTTTAATTCTACTTAATATTTTAATGTGTTTAAGGATTTAT
CTGAATGTATTTTTTAGGATGACTTTAAAATATAATCAGATTTTGTATATTGCTCTTATT
CAATTTTTAGGTTTGCTGATAGGACTATTTCTATATTATTTAACCCAAAACTGGATTGTT
TGTTTTATTACCAGTGAATTGTTTGCAACGATATATACATTGGTTAAATTACGGGGATTA
ACTATAGGCGAGTATCAAAGTGAAGATAATAATGTGGTAAAAGATTATGTGATGCTACTG
AGTACAAATAGCCTTAATAATTTGAATCTCTATTTAGATAGATTAATCTTATTACCAATT
ATAGGTGGAACAGCTGTAACTATATCATTTCTTTCAACATTTATTGGGAAAATGTTAGCT
ACATTTCTATATCCGATTAATAATGTAGTACTTTCATATATTTCTGTAAATGAAAGTGAC
AATATAAAGAAGCAATATTTGAAAACTAATCTAATTGCTATAGCTGCCCTATGTTTAGTC
ATGATTATATGTTATCCAATTACAATAATTATTGTCTCTTTACTGTATAACATTGATTCA
AGTTTATATTCGAAGTTTATTATTTTAGGTAATATAGGTGTTTTATTCAATGCAGTGAGT
ATTATGATCCAAACTTTAAATACAAAACACGCATCAATAACATTACAAGCGAATTATATG
ACGCTTCACACGATTACATTTATATTCATAACTATTTTAATGACAATTGCGTTTGGTCTA
AATGGATTCTTTTGGACAACGCTGTTCAGCAACATTATTAAGTATGTGATTTTAAATATT
ATAGGTTTAAAGTCTAAATTCATTAATAAAAAGGACGTCGATTAG60
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⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: NWMN_0105 [new locus tag: NWMN_RS00585 ]
- symbol: CapK
- description: capsular polysaccharide biosynthesis protein CapK
- length: 394
- theoretical pI: 9.30526
- theoretical MW: 44854.6
- GRAVY: 0.957107
⊟Function[edit source | edit]
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors:
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 12
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular Possibility: 0
- Signal Peptide Possibility: -0.5
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- Ymax: 0.143
- Ymax_pos: 22
- Cmax: 0.142
- Cmax_pos: 36
- Smax: 0.29
- Smax_pos: 19
- Smean: 0.197
- D: 0.164
- predicted transmembrane helices (TMHMM): 10
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSILTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFASILLISILIESIALIIVFLYFFNLNIIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLFLYYLTQNWIVCFITSELFATIYTLVKLRGLTIGEYQSEDNNVVKDYVMLLSTNSLNNLNLYLDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSYISVNESDNIKKQYLKTNLIAIAALCLVMIICYPITIIIVSLLYNIDSSLYSKFIILGNIGVLFNAVSIMIQTLNTKHASITLQANYMTLHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKSKFINKKDVD
⊟Experimental data[edit source | edit]
- experimentally validated: no data available
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- MicrobesOnline: capA > capB > capC > capD > capE > capF > capG > capH > capI > capJ > capK > capL > capM > capN > capO > capP
⊟Regulation[edit source | edit]
- sigma factor: SigB (activation) regulon [1] other strains
SigB (sigma factor) controlling a large regulon involved in stress/starvation response and adaptation [1] - regulator: CodY (repression) regulon
CodY (TF) important in Amino acid metabolism; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit source | edit]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
- ↑ 1.0 1.1
Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
J. Bacteriol.: 2011, 193(18);4954-62
[PubMed:21725011] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit source | edit]
