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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1368 [new locus tag: NWMN_RS07705 ]
  • pan locus tag?: SAUPAN003917000
  • symbol: NWMN_1368
  • pan gene symbol?: papS
  • synonym:
  • product: tRNA CCA-pyrophosphorylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1368 [new locus tag: NWMN_RS07705 ]
  • symbol: NWMN_1368
  • product: tRNA CCA-pyrophosphorylase
  • replicon: chromosome
  • strand: -
  • coordinates: 1534444..1535646
  • length: 1203
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGATAAATCATTATTTGAACAGGCAAGGCCTATATTAGAACAAATTCAAGACAATGGT
    TTTGAAGCATATTATGTAGGTGGCTCTGTAAGAGATTATGTCATGGGAAGAAATATTCAT
    GATATAGATATCACAACAAGTGCAACGCCGGATGAAATAGAATCTATCTTTAGTCATACG
    ATACCTGTAGGTAAAGAACATGGCACGATAAATGTAGTTTTTAATGATGAAAATTATGAA
    GTGACAACATTCCGGGCTGAAGAAGATTATGTCGATCACCGTAGACCAAGTGGTGTTACA
    TTTGTTCGTGATTTATACGAAGATTTGCAACGACGAGATTTCACGATGAATGCGATAGCA
    ATGGATACAGCATACAAATTGTATGATTATTTTGATGGTCAACAAGATATTAATAATCGA
    ATAATAAGAACTGTAGGTATAGCTGAGGAACGATTCCAAGAAGATGCTTTACGTATGATT
    CGATGTTTAAGGTTCCAGTCACAATTATCATTTGATATTGCAACGGAAACATTCGAAGCG
    ATGCGTATACAAATGGCAGATATTAAATTTTTATCAATTGAGCGTATAGTGATTGAACTA
    ACTAAATTAATGCGAGGTATTAATGTTGAAAAGAGTTTTAATCATTTAAAATCGCTGAAA
    GCATTTAATTATATGCCGTATTTCGAACATCTTGATATGAATCAAATTAATGTAACTGAA
    GCAATTGATTTAGAATTGTTGATTGCTATAGTATCAGTTAAATTTGATATTAATTACTCA
    TTGAAGCCTTTAAAGCTAAGTAACCGACAAGTAAAAGATATCAATCAATATATTCAAATT
    ATGAATGCATTACCAAGTATTATTACAAAAGAACAATTAAAAATGTTTGTTTATGATTAT
    GATACGCATCTCATTAAAAATGTAATGGTTGCAGCAGACGTGTTAAAGGCAAATGATATT
    CAAGGACATGAACCATTAATCGTTAACCTTCAAACGATTGATGAAACATTACATCGTTTA
    CCTATGCATAATAGAAAAGACATGATGGTTAATGGCGGTGTACTTATGGCACATTTAAAT
    GCCAAAAGTGGTCCGTGGTTAAAAGATGTGCTAAGACAAATTGAGATAGCGATTGTAACA
    GGTAAAGTAAGCAACGAAGAAACTGAAATTTTGAAATGGGTGGATAATCATGTCAAAATA
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1203

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1368 [new locus tag: NWMN_RS07705 ]
  • symbol: NWMN_1368
  • description: tRNA CCA-pyrophosphorylase
  • length: 400
  • theoretical pI: 5.1044
  • theoretical MW: 46435.9
  • GRAVY: -0.247

Function[edit source | edit]

  • reaction:
    EC 2.7.7.72?  ExPASy
    CCA tRNA nucleotidyltransferaseA tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate
  • TIGRFAM:
    poly(A) polymerase (TIGR01942; EC 2.7.7.19; HMM-score: 161.7)
    Genetic information processingProtein synthesistRNA and rRNA base modificationCCA tRNA nucleotidyltransferase (TIGR02692; EC 2.7.7.72; HMM-score: 161.1)
  • TheSEED:  
    RNA MetabolismRNA processing and modificationtRNA nucleotidyltransferase tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25) 
  • PFAM:
    NTP_transf (CL0260) PolyA_pol; Poly A polymerase head domain (PF01743; HMM-score: 125.7)
    HD_PDEase (CL0237) tRNA_NucTran2_2; tRNA nucleotidyltransferase domain 2 putative (PF13735; HMM-score: 83.8)
    no clan definedPolyA_pol_RNAbd; Probable RNA and SrmB- binding site of polymerase A (PF12627; HMM-score: 34.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.105
    • Ymax_pos: 24
    • Cmax: 0.123
    • Cmax_pos: 24
    • Smax: 0.134
    • Smax_pos: 18
    • Smean: 0.074
    • D: 0.093
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMIRCLRFQSQLSFDIATETFEAMRIQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTEAIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIITKEQLKMFVYDYDTHLIKNVMVAADVLKANDIQGHEPLIVNLQTIDETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]