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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1369 [new locus tag: NWMN_RS07710 ]
  • pan locus tag?: SAUPAN003918000
  • symbol: NWMN_1369
  • pan gene symbol?: bshA
  • synonym:
  • product: glycosyl transferase, group 1 family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1369 [new locus tag: NWMN_RS07710 ]
  • symbol: NWMN_1369
  • product: glycosyl transferase, group 1 family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1535651..1536793
  • length: 1143
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAGATAGGTATAACATGTTACCCGTCCATGGGTGGTTCTGGAATTATTGCCACAGAA
    TTAGGAATTAAATTAGCAGAGCGAGGTCACGAAGTACATTTTATCACTTCAAATATCCCG
    TTTAGAATAAGAAAACCATTACCGAATATGATTTTTCATCAAGTTGAAGTGAATCAATAT
    GCAGTATTTCAGTATCCACCGTATGATATTACGTTAAGTACTAAAATCGCTGAAGTTATT
    AAAGAATATGATCTTGATTTGTTGCATATGCACTATGCTGTACCTCATGCAATTTGTGGG
    ATTTTAGCTCGTGAGATGTCAGGTAAAGATATAAAAATTATGACAACGCTACACGGTACT
    GATATTACTGTTTTGGGCTATGATCATTCACTCCAAGGTGCAATTAAATTTGGCATTGAA
    AAAAGTGATATTGTGACAAGTGTGAGTAAATCTTTAGCACAAGAAACACATGAAATTATT
    GAAACTAACAAAGAAATTATACCGATTTATAATTTTGTACGTGAAAATGAGTTTCCAACT
    AAACATAACACAGCATTAAAATCACAATTTGGTATAGCACCAGATGAAAAAGTGTTAATA
    CATGTATCGAATTTTAGACAAGTAAAACGCATAGATACTATTATAGAAACATTTGCAAAA
    GTACGTGAAAAGATACCTAGTAAATTAATTTTACTTGGAGATGGTCCTGAATTAGTGCCA
    ATGCGTCAATTGACGAAAGAGTTAAACGTAGAAGAGGATGTTTTATTTTTAGGGAAACAA
    GATTGCGTAAGTGAATTTTACCAATTATCTGACTTAGTATTATTATTAAGTGAAAAAGAA
    AGCTTTGGACTAACTTTACTTGAGGCTATGAAAACAGGCGTCGTTCCAATTGGTTCAAAT
    GCCGGTGGAATTAAAGAGGTTATTAAACATGGTGAAACTGGATTTGTCGTAGATGTGGGT
    GATTGTGATTCAGCGAGTGACTATGCTATCCGATTACTTGAAGATAAGGTGTTATACAAC
    AAACTTCAGAAAAACATGCTAGCAGATATTGCTGAACGTTTTGGATCAGAACTTATTACA
    GATCAATATGAGTATTATTATCAAAAAATGCTAAATGAGCATAACAAAAGTAAAGGCGAG
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1143

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1369 [new locus tag: NWMN_RS07710 ]
  • symbol: NWMN_1369
  • description: glycosyl transferase, group 1 family protein
  • length: 380
  • theoretical pI: 5.75583
  • theoretical MW: 42795.1
  • GRAVY: -0.127632

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsN-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 553.2)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 93.2)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 78.9)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 75.7)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 61.4)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 61.3)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 54.8)
    Genetic information processingProtein fateProtein modification and repairaccessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 41.9)
    Unknown functionEnzymes of unknown specificityglycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 38.9)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidessucrose synthase (TIGR02470; EC 2.4.1.13; HMM-score: 30.1)
    colanic acid biosynthesis glycosyltransferase WcaI (TIGR04007; EC 2.4.-.-; HMM-score: 29.7)
    sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 26.9)
    sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 25.3)
    colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 24)
  • TheSEED:  
    Cell Wall and CapsuleCapsular and extracellular polysacchridesExopolysaccharide Biosynthesis Glycosyl transferase, group 1 family protein 
  • PFAM:
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 128.6)
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 84.6)
    Glyco_transf_4; Glycosyltransferase Family 4 (PF13439; HMM-score: 70.7)
    Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 47)
    Glyco_trans_4_4; Glycosyl transferase 4-like domain (PF13579; HMM-score: 29.4)
    Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 24.1)
    Glyco_transf_5; Starch synthase catalytic domain (PF08323; HMM-score: 22.2)
    NADP_Rossmann (CL0063) Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 14.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.178
    • Ymax_pos: 19
    • Cmax: 0.222
    • Cmax_pos: 19
    • Smax: 0.271
    • Smax_pos: 3
    • Smean: 0.169
    • D: 0.174
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIRKPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGILAREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEIIETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETFAKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLLSEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLEDKVLYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]