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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1390 [new locus tag: NWMN_RS07845 ]
  • pan locus tag?: SAUPAN003947000
  • symbol: NWMN_1390
  • pan gene symbol?: recQ2
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1390 [new locus tag: NWMN_RS07845 ]
  • symbol: NWMN_1390
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1558145..1559524
  • length: 1380
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTTGCATGATATTTTACGAAACAAATTTGGATTCGAGAGTTTTAAACCGGGACAACAG
    GAAATTATAGAAAGTATAATGTCTCAACAACACACTCTAGGTATACTTCCAACTGGAAGT
    GGAAAGAGTTTGTGTTATCAAATACCTACGTATTTATCAGGTAAGCCGACATTAATTATC
    TCACCGTTAATATCTTTAATGGATGACCAAGTTATGCAGTTGAAAATAAATGGAGAAAAA
    CGTGTAACATGTATTCACTCTGGTATGGATGAAATTGAGAAAAAGCATAATATTAAATGT
    TTACGACATAGCCGCTTCATCTTTCTAAGTCCAGAATTTCTCCTGCAACCGTCAAATTTT
    AAATTAATATCTATGATAGACTTTGGCATGATTGTTCTAGATGAAGCACATTGCCTATCT
    GAATGGGGATATGATTTCAGACCACATTATGCTCTAATAGGAAAAGTAACAAAGCATTTT
    AAAGAAGCGGTTGTCTTAGCATTGACAGCAACTGCACCACCGCATTTACAAGATGATTTG
    ACGGAAATGTTAGCGATTCAATTCAATGTTATTAAAACTACAATGAATCGCCCAAATATA
    AGCTTTAAGCATCTTAATTTTCATGATGATGAAGATAAAATTGAATGGTTGCTGCCGTTT
    CTACAACAGTCGGGACCAACGATTATTTATGTCTCATCGAAAAAGATGTGTCTGAATTTA
    GCGCAACTTATTTATGATTCAGGTTTTCTTACAGGTATTTATCATGGTGATATGAATTAT
    CAAGAGCGACACACAGTTCAACAACAATTTTTAAATAATGATATTCCGATTATAGTCGCA
    ACGAGTGCTTTTGGTATGGGAATTAATAAAAAAGATATTCGCACAATCATTCACTTTCAT
    CTTTCAACAAGTCCTTCTAACTACATTCAAGAAATTGGCCGTGCGGGTCGCGATGGTGAA
    CTAAGTCAGGCAATTAGTTTATTCCAACCGGACGATAAATATATTTTAGAAACGTTATTA
    TTTGCAGATATGATAACAGAAGAAGATGTACAAAATTTCGAAATAGGAGAATTTTTAGCT
    CCCGATAAACAAGCCGTTTTGACAACGTTGCAATCATTCTATAGTATCGGCGCCTTGAAA
    CAGATATTTAAGCAATCATTTAAACGAAAGCAATTAGGATTCTTTCGCATGATTGGCTAT
    TGCAAATTGGATCAATGTAGACGGAAGTATTTATTAGAATTTTTCGGTGAATATCCACCG
    GCACAAGATCGATGTTGTGACAATGATTCTAATATAACTGATATCGCAATTTTAAATAAG
    AAGAAGGTAATTAGAAGTATTGGATTTGATGAAAAGTTGCAAAATTTATTTCTCAGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1390 [new locus tag: NWMN_RS07845 ]
  • symbol: NWMN_1390
  • description: hypothetical protein
  • length: 459
  • theoretical pI: 7.04867
  • theoretical MW: 52730.7
  • GRAVY: -0.163617

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 346.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 339)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 44.4)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 29.5)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairreverse gyrase (TIGR01054; EC 3.6.4.12,5.99.1.3; HMM-score: 22.5)
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 17.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 16.9)
    CRISPR-associated helicase Cas3, subtype CYANO (TIGR03158; HMM-score: 13.8)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairtranscription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 13.3)
    glutaredoxin-like protein NrdH (TIGR02194; HMM-score: 12.6)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairputative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 12.4)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexcinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 11.9)
  • TheSEED:  
    DNA MetabolismDNA repairDNA repair, bacterial RecFOR pathway ATP-dependent DNA helicase, RecQ family 
  • PFAM:
    P-loop_NTPase (CL0023) Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 84.6)
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 72)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 32.1)
    no clan definedRecQ_Zn_bind; RecQ zinc-binding (PF16124; HMM-score: 31.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_0961(pdhC)branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    NWMN_1166(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_1613(rpsD)30S ribosomal protein S4  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_1391hypothetical protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 70
    • Cmax: 0.107
    • Cmax_pos: 55
    • Smax: 0.148
    • Smax_pos: 1
    • Smean: 0.093
    • D: 0.101
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLHDILRNKFGFESFKPGQQEIIESIMSQQHTLGILPTGSGKSLCYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLRHSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHFKEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLLPFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIPIIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKYILETLLFADMITEEDVQNFEIGEFLAPDKQAVLTTLQSFYSIGALKQIFKQSFKRKQLGFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIAILNKKKVIRSIGFDEKLQNLFLR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]