From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1964 [new locus tag: NWMN_RS11335 ]
  • pan locus tag?: SAUPAN005308000
  • symbol: leuB
  • pan gene symbol?: leuB
  • synonym:
  • product: 3-isopropylmalate dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1964 [new locus tag: NWMN_RS11335 ]
  • symbol: leuB
  • product: 3-isopropylmalate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 2175894..2176940
  • length: 1047
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACTTATAACATTGTTGCCCTACCTGGTGATGGAATCGGTCCAGAAATTTTGAACGGA
    TCTCTATCATTGCTTGAAATTATAAGTAATAAATATAACTTTAATTATCAAATAGAGCAC
    CACGAATTTGGTGGTGCCTCTATTGATACATTCGGCGAGCCTTTAACTGAGAAAACCTTA
    AATGCGTGTAAAAGAGCAGATGCTATTTTACTGGGTGCAATCGGTGGACCTAAATGGACA
    GATCCTAACAATCGACCAGAACAAGGATTATTAAAATTGCGTAAATCCTTAAATTTATTT
    GTAAATATACGCCCCACTACCGTTGTCAAAGGCGCTAGTTCTTTATCACCTTTAAAGGAA
    GAACGCGTTGAAGGCACAGATTTAGTTATAGTCCGTGAATTGACAAGTGGTATTTATTTT
    GGAGAACCTAGACATTTTAATAATCACGAGGCCTTAGATTCTCTTACTTATACAAGAGAA
    GAAATAGAACGCATTGTTCACGTAGCATTTAAATTGGCCGCTTCAAGACGAGGAAAACTA
    ACATCAGTTGATAAAGAAAATGTATTAGCTTCTAGTAAATTGTGGCGCAAAGTCGTAAAT
    GAAGTAAGTCAATTATATCCAGAAGTAACAGTAAATCACTTACTTGTTGATGCTTGTAGT
    ATGCATTTAATCACAAATCCAAAACAATTTGACGTCATCGTATGTGAAAACTTATTTGGC
    GATATTTTAAGTGATGAAGCTTCAGTGATTCCTGGTTCACTTGGTTTATCACCTTCTGCT
    AGTTTTAGTAACGATGGTCCAAGATTGTATGAGCCTATTCATGGATCAGCACCAGATATT
    GCAGGTAAAAACGTTGCCAATCCATTTGGAATGATTCTATCTTTAGCGATGTGTTTACGT
    GAAAGCTTAAATCAACCAGATGCTGCAGATGAATTAGAACAACATATTTATAGCATGATT
    GAACATGGGCAAACGACAGCAGATTTAGGCGGCAAATTGAATACTACTGATATTTTCGAA
    ATTCTATCTCAAAAATTGAATCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1047

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1964 [new locus tag: NWMN_RS11335 ]
  • symbol: LeuB
  • description: 3-isopropylmalate dehydrogenase
  • length: 348
  • theoretical pI: 5.24197
  • theoretical MW: 38213.1
  • GRAVY: -0.173563

Function[edit source | edit]

  • reaction:
    EC 1.1.1.85?  ExPASy
    3-isopropylmalate dehydrogenase(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH
  • TIGRFAM:
    MetabolismAmino acid biosynthesisPyruvate family3-isopropylmalate dehydrogenase (TIGR00169; EC 1.1.1.85; HMM-score: 470.2)
    isopropylmalate/isohomocitrate dehydrogenases (TIGR02088; HMM-score: 292.5)
    MetabolismEnergy metabolismOthertartrate dehydrogenase (TIGR02089; EC 1.1.1.93; HMM-score: 244.7)
    MetabolismEnergy metabolismTCA cycleisocitrate dehydrogenase (TIGR02924; EC 1.1.1.-; HMM-score: 182.2)
    MetabolismEnergy metabolismTCA cycleisocitrate dehydrogenase, NAD-dependent (TIGR00175; EC 1.1.1.41; HMM-score: 178.4)
    MetabolismEnergy metabolismTCA cycleisocitrate dehydrogenase, NADP-dependent (TIGR00183; EC 1.1.1.42; HMM-score: 108)
    MetabolismEnergy metabolismTCA cycleisocitrate dehydrogenase, NADP-dependent (TIGR00127; EC 1.1.1.42; HMM-score: 11.2)
  • TheSEED:  
    Amino Acids and DerivativesBranched-chain amino acidsBranched-Chain Amino Acid Biosynthesis 3-isopropylmalate dehydrogenase (EC 1.1.1.85) 
    Branched-chain amino acidsLeucine Biosynthesis 3-isopropylmalate dehydrogenase (EC 1.1.1.85) 
  • PFAM:
    Iso_DH (CL0270) Iso_dh; Isocitrate/isopropylmalate dehydrogenase (PF00180; HMM-score: 410.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.112
    • Ymax_pos: 36
    • Cmax: 0.113
    • Cmax_pos: 63
    • Smax: 0.143
    • Smax_pos: 30
    • Smean: 0.1
    • D: 0.108
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFGGASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFVNIRPTTVVKGASSLSPLKEERVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTREEIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDACSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGSAPDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYSMIEHGQTTADLGGKLNTTDIFEILSQKLNH

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolismRegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]