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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2446 [new locus tag: NWMN_RS14060 ]
  • pan locus tag?: SAUPAN006207000
  • symbol: mvaS
  • pan gene symbol?: mvaS
  • synonym:
  • product: 3-hydroxy-3-methylglutaryl coenzyme A synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2446 [new locus tag: NWMN_RS14060 ]
  • symbol: mvaS
  • product: 3-hydroxy-3-methylglutaryl coenzyme A synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 2688971..2690137
  • length: 1167
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGACAATAGGTATCGATAAAATAAACTTTTACGTTCCAAAGTACTATGTAGACATGGCT
    AAATTAGCAGAAGCACGCCAAGTAGACCCAAACAAATTTTTAATTGGAATTGGTCAAACT
    GAAATGGCTGTTAGTCCTGTAAACCAAGACATCGTTTCAATGGGCGCTAACGCTGCTAAG
    GACATTATAACAGACGAAGACAAAAAGAAAATTGGTATGGTAATTGTGGCAACTGAATCA
    GCAGTTGATGCTGCTAAAGCAGCCGCTGTTCAAATTCACAACTTATTAGGTATTCAACCT
    TTTGCACGCTGCTTTGAAATGAAAGAAGCTTGTTATGCTGCAACACCAGCAATTCAATTA
    GCTAAAGATTATTTAGCAACTAGACCGAATGAAAAAGTATTAGTTATTGCTACAGATACA
    GCACGTTATGGATTGAATTCAGGCGGCGAGCCAACACAAGGTGCTGGCGCAGTTGCGATG
    GTTATTGCACATAATCCAAGCATTTTGGCATTAAATGAAGATGCTGTTGCTTACACTGAA
    GACGTTTATGATTTCTGGCGTCCAACTGGACATAAATATCCATTAGTTGATGGTGCATTA
    TCTAAAGATGCTTATATCCGCTCATTCCAACAAAGCTGGAATGAATACGCAAAACGTCAA
    GGTAAGTCGCTAGCTGACTTCGCATCTCTATGCTTCCATGTTCCATTTACAAAAATGGGT
    AAAAAGGCATTAGAGTCAATCATTGATAACGCTGATGAAACAACTCAAGAGCGTTTACGT
    TCAGGATATGAAGATGCTGTAGATTATAACCGTTATGTCGGTAATATTTATACTGGATCA
    TTATATTTAAGCCTAATATCATTACTTGAAAATCGAGATTTACAAGCTGGTGAAACAATC
    GGTTTATTCAGTTATGGCTCAGGTTCAGTTGGTGAATTTTATAGTGCGACATTAGTTGAA
    GGCTACAAAGATCATTTAGATCAAGCTGCACATAAAGCATTATTAAATAACCGTACTGAA
    GTATCTGTTGATGCATATGAAACATTCTTCAAACGTTTTGATGACGTTGAATTTGACGAA
    GAACAAGATGCTGTTCATGAAGATCGTCATATTTTCTACTTATCAAATATTGAAAATAAC
    GTTCGTGAATATCACAGACCAGAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1167

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2446 [new locus tag: NWMN_RS14060 ]
  • symbol: MvaS
  • description: 3-hydroxy-3-methylglutaryl coenzyme A synthase
  • length: 388
  • theoretical pI: 4.67485
  • theoretical MW: 43205.2
  • GRAVY: -0.295361

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    hydroxymethylglutaryl-CoA synthase (TIGR01835; EC 2.3.3.10; HMM-score: 531.4)
    hydroxymethylglutaryl-CoA synthase (TIGR01833; EC 2.3.3.10; HMM-score: 160.5)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisputative hydroxymethylglutaryl-CoA synthase (TIGR00748; EC 2.3.3.10; HMM-score: 98.4)
    MetabolismFatty acid and phospholipid metabolismBiosynthesis3-oxoacyl-[acyl-carrier-protein] synthase III (TIGR00747; EC 2.3.1.180; HMM-score: 43.1)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsIsoprenoidsArchaeal lipids Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10) 
    IsoprenoidsIsoprenoid Biosynthesis Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10) 
  • PFAM:
    Thiolase (CL0046) HMG_CoA_synt_C; Hydroxymethylglutaryl-coenzyme A synthase C terminal (PF08540; HMM-score: 96.8)
    HMG_CoA_synt_N; Hydroxymethylglutaryl-coenzyme A synthase N terminal (PF01154; HMM-score: 69.1)
    Chal_sti_synt_N; Chalcone and stilbene synthases, N-terminal domain (PF00195; HMM-score: 27)
    ACP_syn_III; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III (PF08545; HMM-score: 26.1)
    ACP_syn_III_C; 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal (PF08541; HMM-score: 17.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_1580(gapB)glyceraldehyde 3-phosphate dehydrogenase 2  [1] (data from MRSA252)
    NWMN_1348(ilvA)threonine dehydratase  [1] (data from MRSA252)
    NWMN_2438(poxB)pyruvate oxidase  [1] (data from MRSA252)
    NWMN_0463(prs)ribose-phosphate pyrophosphokinase  [1] (data from MRSA252)
    NWMN_1658(putA)proline dehydrogenase  [1] (data from MRSA252)
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_1166(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_2146(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_0641hypothetical protein  [1] (data from MRSA252)
    NWMN_0811hypothetical protein  [1] (data from MRSA252)
    NWMN_1604universal stress protein family protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.122
    • Ymax_pos: 59
    • Cmax: 0.145
    • Cmax_pos: 59
    • Smax: 0.137
    • Smax_pos: 22
    • Smean: 0.095
    • D: 0.111
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAVSPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFARCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAMVIAHNPSILALNEDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAKRQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNIYTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKALLNNRTEVSVDAYETFFKRFDDVEFDEEQDAVHEDRHIFYLSNIENNVREYHRPE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • mvaS no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]