From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1348 [new locus tag: NWMN_RS07605 ]
  • pan locus tag?: SAUPAN003892000
  • symbol: ilvA
  • pan gene symbol?: ilvA1
  • synonym:
  • product: threonine dehydratase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1348 [new locus tag: NWMN_RS07605 ]
  • symbol: ilvA
  • product: threonine dehydratase
  • replicon: chromosome
  • strand: -
  • coordinates: 1510842..1511882
  • length: 1041
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACAACCAACACAGTTACATTACAAACAGCACATATTGTAAGTTTAGGAGATATCGAA
    GAAGCTAAAGCAAGCATTAAACCATTTATTCGTCGAACACCTCTAATTAAATCAATGTAT
    TTAAGCCAAAGTATAACTAAAGGGAATGTATTTCTAAAATTAGAAAATATGCAATTCACA
    GGATCTTTTAAATTTAGAGGCGCTAGCAATAAAATTAATCACTTAACAGATGAACAAAAA
    GAAAAAGGCATTATCGCAGCATCTGCTGGGAACCATGCACAAGGTGTTGCTTTAACAGCT
    AAATTATTAGGCATTGATGCAACGATTGTAATGCCTGAAACAGCACCACAAGCGAAACAA
    CAAGCAACAAAAGGCTATGGGGCAAAGGTTATTTTAAAAGGTAAAAACTTTAACGAAACT
    AGACTTTATATGGAAGAATTAGCGAAAGAAAATGGCATGACAATCGTTCATCCATATGAC
    GATAAGTTTGTAATGGCAGGCCAAGGAACAATTGGTTTAGAAATTTTAGATGATATTTGG
    AATGTGAATACAGTCATCGTACCAGTTGGCGGTGGAGGATTAATTGCAGGTATTGCCACC
    GCATTAAAATCATTTAACCCTTCAATTCATATTATCGGTGTTCAATCTGAGAATGTTCAT
    GGTATGGCTGAGTCTTTCTATAAGAGAGATTTAACTGAACATCGAGTGGATAGCACAATA
    GCAGATGGTTGTGATGTAAAAGTTCCTGGTGAACAAACATATGAAGTAGTTAAACATTTA
    GTAGATGAATTTATTCTTGTTACTGAAGAAGAAATTGAACATGCTATGAAAGATTTAATG
    CAGCGTGCCAAAATTATTACTGAAGGTGCAGGCGCATTACCAACAGCTGCAATTTTAAGT
    GGAAAAATAAACAATAAATGGCTTGAAGATAAAAATGTTGTTGCATTAGTTTCAGGCGGG
    AATGTTGACTTAACTAGAGTTTCAGGTGTCATTGAACATGGACTGAATATTGCAGATACA
    AGCAAGGGTGTGGTAGGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1041

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1348 [new locus tag: NWMN_RS07605 ]
  • symbol: IlvA
  • description: threonine dehydratase
  • length: 346
  • theoretical pI: 6.51894
  • theoretical MW: 37305.6
  • GRAVY: -0.0575145

Function[edit source | edit]

  • reaction:
    EC 4.3.1.19?  ExPASy
    Threonine ammonia-lyaseL-threonine = 2-oxobutanoate + NH3
  • TIGRFAM:
    MetabolismAmino acid biosynthesisPyruvate familythreonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 395.5)
    MetabolismAmino acid biosynthesisPyruvate familythreonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 301.5)
    MetabolismAmino acid biosynthesisPyruvate familythreonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 266.5)
    ectoine utilization protein EutB (TIGR02991; HMM-score: 263.4)
    MetabolismAmino acid biosynthesisSerine familycysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 94.6)
    MetabolismAmino acid biosynthesisSerine familycysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 81.6)
    MetabolismAmino acid biosynthesisAspartate familythreonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 74.2)
    MetabolismAmino acid biosynthesisSerine familycysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 71.9)
    Cellular processesCellular processesBiosynthesis of natural products2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 68.5)
    MetabolismAmino acid biosynthesisSerine familycystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 60.9)
    diaminopropionate ammonia-lyase (TIGR03528; EC 4.3.1.15; HMM-score: 56)
    MetabolismEnergy metabolismOtherdiaminopropionate ammonia-lyase family (TIGR01747; EC 4.3.1.15; HMM-score: 49.7)
    MetabolismAmino acid biosynthesisAromatic amino acid familytryptophan synthase, beta subunit (TIGR00263; EC 4.2.1.20; HMM-score: 30.7)
    pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family (TIGR01275; HMM-score: 29.8)
    Unknown functionEnzymes of unknown specificitypyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 28.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 22.9)
    MetabolismEnergy metabolismMethanogenesiscysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 22.9)
  • TheSEED:  
    Amino Acids and DerivativesAlanine, serine, and glycineGlycine and Serine Utilization Threonine dehydratase, catabolic (EC 4.3.1.19) 
    Branched-chain amino acidsBranched-Chain Amino Acid Biosynthesis Threonine dehydratase, catabolic (EC 4.3.1.19) 
    Lysine, threonine, methionine, and cysteineThreonine degradation Threonine dehydratase, catabolic (EC 4.3.1.19) 
    Branched-Chain Amino Acid Biosynthesis L-serine dehydratase, (PLP)-dependent (EC 4.3.1.17) 
  • PFAM:
    no clan definedPALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 275.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_0372(ahpC)alkyl hydroperoxide reductase subunit C  [1] (data from MRSA252)
    NWMN_2534(arcA)arginine deiminase  [1] (data from MRSA252)
    NWMN_1587(citC)isocitrate dehydrogenase  [1] (data from MRSA252)
    NWMN_0745(eno)phosphopyruvate hydratase  [1] (data from MRSA252)
    NWMN_1096(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_0741(gapA)glyceraldehyde 3-phosphate dehydrogenase 1  [1] (data from MRSA252)
    NWMN_1580(gapB)glyceraldehyde 3-phosphate dehydrogenase 2  [1] (data from MRSA252)
    NWMN_2056(glmS)glucosamine--fructose-6-phosphate aminotransferase  [1] (data from MRSA252)
    NWMN_0176(ldh1)L-lactate dehydrogenase  [1] (data from MRSA252)
    NWMN_1680(metK)S-adenosylmethionine synthetase  [1] (data from MRSA252)
    NWMN_2446(mvaS)3-hydroxy-3-methylglutaryl coenzyme A synthase  [1] (data from MRSA252)
    NWMN_0961(pdhC)branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    NWMN_0962(pdhD)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    NWMN_0162(pflB)formate acetyltransferase  [1] (data from MRSA252)
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_0500(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_2149(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    NWMN_2137(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    NWMN_0501(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    NWMN_0499(rplK)50S ribosomal protein L11  [1] (data from MRSA252)
    NWMN_0502(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    NWMN_2133(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_1549(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    NWMN_2147(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    NWMN_2150(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    NWMN_1166(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_2146(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    NWMN_1613(rpsD)30S ribosomal protein S4  [1] (data from MRSA252)
    NWMN_2135(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    NWMN_2143(rpsQ)30S ribosomal protein S17  [1] (data from MRSA252)
    NWMN_1325(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    NWMN_1167(tsf)elongation factor Ts  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)
    NWMN_0641hypothetical protein  [1] (data from MRSA252)
    NWMN_0811hypothetical protein  [1] (data from MRSA252)
    NWMN_1382DNA-binding protein HU  [1] (data from MRSA252)
    NWMN_1604universal stress protein family protein  [1] (data from MRSA252)
    NWMN_2086alkaline shock protein 23  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.78
    • Cytoplasmic Membrane Score: 8.16
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.228
    • Ymax_pos: 25
    • Cmax: 0.312
    • Cmax_pos: 25
    • Smax: 0.208
    • Smax_pos: 21
    • Smean: 0.151
    • D: 0.198
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQSITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKLLGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYDDKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSENVHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAMKDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHGLNIADTSKGVVG

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]