From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2595 [new locus tag: NWMN_RS14875 ]
  • pan locus tag?: SAUPAN006459000
  • symbol: NWMN_2595
  • pan gene symbol?: nixA
  • synonym:
  • product: high-affinity nickel transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_2595 [new locus tag: NWMN_RS14875 ]
  • symbol: NWMN_2595
  • product: high-affinity nickel transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 2862506..2863558
  • length: 1053
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATTAAATATCATGTTATGAAAGAAGGATATATTTTGACTGTTTTTAAAAATGAGCGC
    TTAAGCTGGTTACCATACATAGCTATTGTCATTTTGTTACACGTTATTGGGTTTAGTTTT
    TTATGGATTGCTGGAAAAGACCATCATATCTTATTTGGTATGGGGATTCTTGCATATACA
    TTAGGTTTGCGTCATGCATTTGATGCAGATCACATTGCTGCAATAGATAATACGGTTCGC
    AAATTATTACAGCAACGCAAAGATCCATCTGGTGTGGGTTTCTATTTTTCAATTGGACAT
    TCATCTGTCGTATTTTTAATGGCTGTTTTTTTAGGGGTATCTGTAAAATGGGCTAAAGAT
    GAATTACCGCATTTCCAAGATATTGGTGGAACGATTGGTACACTAGTTTCAGGTTTCTTT
    TTAGTGCTTATCGGTGTGTTGAATCTAATTATTTTAATCTCTTTAATTAACTTGTTCGCT
    AAATTACGTCGTGAACACATTGAAGAAGCTGAAGTCGATGCATTACTTGAATCTAGAGGA
    TTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTAATCACGCGTAGTTGGCACGTATTG
    CCACTTGGCTTTTTATTTGGACTTGGTTTTGATACAGCTAGTGAAATTGCGTTACTCGCT
    CTTTCTTCAGGCGCATCACAACAAGCCATTTCATTTATCGGAATCTTATCTTTACCAATT
    TTATTCGCATCAGGTATGAGTTTATTGGATACATTAGATGGTGTTGTAATGAAGTATGCC
    TATAATTGGGCATTCTTCAATCCTATTCGCAAAATCTATTACAATATAACGATTACTGCG
    ATATCAGTCATGGCAGCATTAGTGATTGGGATGATTGAATTGCTACAAATTCTTGCTGAT
    AAGTTAGATTTACATGGTGCGTTTTGGGCATTCATTGGTTCGATCGAATTTGATTATTTA
    GGCTATATTTTAGTTGCATTATTTTTAATTACTTGGCTTATTTCAAGTTTAATTTGGAAG
    TTTGGTCGCATAGAACACAAATGGTCTAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1053

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_2595 [new locus tag: NWMN_RS14875 ]
  • symbol: NWMN_2595
  • description: high-affinity nickel transporter
  • length: 350
  • theoretical pI: 8.66941
  • theoretical MW: 39305.3
  • GRAVY: 0.743714

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundstransition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family (TIGR00802; HMM-score: 380.8)
  • TheSEED:  
    Membrane TransportMembrane Transport - no subcategoryTransport of Nickel and Cobalt HoxN/HupN/NixA family nickel/cobalt transporter 
  • PFAM:
    LysE (CL0292) NicO; High-affinity nickel-transport protein (PF03824; HMM-score: 335.2)
    no clan definedMHYT; Bacterial signalling protein N terminal repeat (PF03707; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.095
    • Ymax_pos: 11
    • Cmax: 0.175
    • Cmax_pos: 47
    • Smax: 0.153
    • Smax_pos: 66
    • Smean: 0.092
    • D: 0.094
  • predicted transmembrane helices (TMHMM): 8

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIKYHVMKEGYILTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGILAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVSVKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEVDALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAISFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALVIGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEHKWSR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SigB [1] [2] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]