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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_03033
  • pan locus tag?: SAUPAN006459000
  • symbol: SAOUHSC_03033
  • pan gene symbol?: nixA
  • synonym:
  • product: high affinity nickel transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_03033
  • symbol: SAOUHSC_03033
  • product: high affinity nickel transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 2805006..2806022
  • length: 1017
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGACTGTTTTTAAAAATGAGCGCTTAAGCTGGTTACCATACATAGCTATTGTCATTTTG
    TTACACGTTATTGGGTTTAGTTTTTTATGGATTGCTGGAAAAGACCATCATATCTTATTT
    GGTATGGGGATTCTTGCATATACATTAGGTTTGCGTCATGCATTTGATGCAGATCACATT
    GCTGCAATAGATAATACGGTTCGCAAATTATTACAGCAACGCAAAGATCCATCTGGTGTG
    GGTTTCTATTTTTCAATTGGACATTCATCTGTCGTATTTTTAATGGCTGTTTTTTTAGGG
    GTATCTGTAAAATGGGCTAAAGATGAATTACCGCATTTCCAAGATATTGGTGGAACGATT
    GGTACACTAGTTTCAGGTTTCTTTTTAGTGCTTATCGGTGTGTTGAATCTAATTATTTTA
    ATCTCTTTAATTAACTTGTTCGCTAAATTACGTCGTGAACACATTGAAGAAGCTGAAGTC
    GATGCATTACTTGAATCTAGAGGATTGGTTTCTCGATTTGTAGGACCTTATTTCAAATTA
    ATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTGGACTTGGTTTTGATACA
    GCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTT
    ATCGGAATCTTATCTTTACCAATTTTATTCGCATCAGGTATGAGTTTATTGGATACATTA
    GATGGTGTTGTAATGAAGTATGCCTATAATTGGGCATTCTTCAATCCTATTCGCAAAATC
    TATTACAATATAACGATTACTGCGATATCAGTCATGGCAGCATTAGTGATTGGGATGATT
    GAATTGCTACAAATTCTTGCTGATAAGTTAGATTTACATGGTGCGTTTTGGGCATTCATT
    GGTTCGATCGAATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGG
    CTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1017

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_03033
  • symbol: SAOUHSC_03033
  • description: high affinity nickel transporter
  • length: 338
  • theoretical pI: 8.02909
  • theoretical MW: 37829.5
  • GRAVY: 0.765976

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundstransition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family (TIGR00802; HMM-score: 380.9)
  • TheSEED:  
    Membrane TransportMembrane Transport - no subcategoryTransport of Nickel and Cobalt HoxN/HupN/NixA family nickel/cobalt transporter 
  • PFAM:
    LysE (CL0292) NicO; High-affinity nickel-transport protein (PF03824; HMM-score: 335.3)
    no clan definedMHYT; Bacterial signalling protein N terminal repeat (PF03707; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 11
    • Cmax: 0.201
    • Cmax_pos: 35
    • Smax: 0.19
    • Smax_pos: 54
    • Smean: 0.114
    • D: 0.11
  • predicted transmembrane helices (TMHMM): 7

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGILAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVSVKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEVDALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAISFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALVIGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEHKWSR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SAOUHSC_02298 (SigB*) [1] [2] other strains
    SigB* (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  2. 2.0 2.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]