From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2489 [new locus tag: SA_RS14235 ]
  • pan locus tag?: SAUPAN006459000
  • symbol: SA2489
  • pan gene symbol?: nixA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA2489 [new locus tag: SA_RS14235 ]
  • symbol: SA2489
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2800211..2801227
  • length: 1017
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGACTGTTTTTAAAAATGAGCGCTTAAGCTGGTTACCATACATAGCTATTGTCATTTTG
    TTACACGTTATTGGGTTTAGTTTTTTATGGATTGCTGGAAAAGACCATCATATCTTATTT
    GGTATGGGGATTCTTGCATACACATTAGGTTTGCGTCATGCATTTGATGCAGATCATATC
    GCCGCGATAGACAATACCGTTCGTAAATTATTACAACAACGCAAAGATCCATCTGGTGTG
    GGTTTCTATTTTTCAATTGGACATTCATCTGTTGTATTTTTAATGGCTGTTTTTTTAGGG
    ATATCTGTAAAATGGGCTAAAGATGAATTACCACATTTCCAAGATATTGGTGGAACGATT
    GGTACACTAGTTTCAGGTTTCTTTTTAGTGCTTATCGGTGTGTTGAATCTAATTATTTTA
    ATCTCTTTAATTAACTTGTTCGCTAAATTACGTCGTGAACACATTGAAGAAGCTGAAGTC
    GATGTATTACTTGAATCTAGAGGATTTGTTTCTCGATTTGTAGGACCTTATTTCAAGTTA
    ATCACGCGTAGTTGGCACGTATTGCCACTTGGCTTTTTATTTGGACTTGGTTTTGATACA
    GCTAGTGAAATTGCGTTACTCGCTCTTTCTTCAGGCGCATCACAACAAGCCATTTCATTT
    ATCGGAATCTTATCTTTACCAATTTTATTCGCATCAGGTATGAGTTTATTGGATACATTA
    GATGGTGTTGTAATGAAGTATGCCTATAATTGGGCATTCTTCAATCCTATTCGCAAAATC
    TATTACAATATAACGATTACTGCGATATCAGTCATGGCAGCATTAGTGATTGGGATGATT
    GAATTGCTACAAATTCTTGCTGATAAGCTAGATTTACATGGTGCGTTTTGGGCATTCATT
    GGTTCGATCGAATTTGATTATTTAGGCTATATTTTAGTTGCATTATTTTTAATTACTTGG
    CTTATTTCAAGTTTAATTTGGAAGTTTGGTCGCATAGAACACAAATGGTCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1017

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA2489 [new locus tag: SA_RS14235 ]
  • symbol: SA2489
  • description: hypothetical protein
  • length: 338
  • theoretical pI: 8.02785
  • theoretical MW: 37877.6
  • GRAVY: 0.772781

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundstransition metal uptake transporter, Ni2+-Co2+ transporter (NiCoT) family (TIGR00802; HMM-score: 378.6)
  • TheSEED:  
    Membrane TransportMembrane Transport - no subcategoryTransport of Nickel and Cobalt HoxN/HupN/NixA family nickel/cobalt transporter 
  • PFAM:
    LysE (CL0292) NicO; High-affinity nickel-transport protein (PF03824; HMM-score: 332.5)
    no clan definedMHYT; Bacterial signalling protein N terminal repeat (PF03707; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 11
    • Cmax: 0.201
    • Cmax_pos: 35
    • Smax: 0.19
    • Smax_pos: 54
    • Smean: 0.114
    • D: 0.11
  • predicted transmembrane helices (TMHMM): 7

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTVFKNERLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGILAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGISVKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEVDVLLESRGFVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAISFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALVIGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEHKWSK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]