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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS04080 [old locus tag: NWMN_0722 ]
  • pan locus tag?: SAUPAN002662000
  • symbol: NWMN_RS04080
  • pan gene symbol?: secA
  • synonym:
  • product: preprotein translocase subunit SecA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS04080 [old locus tag: NWMN_0722 ]
  • symbol: NWMN_RS04080
  • product: preprotein translocase subunit SecA
  • replicon: chromosome
  • strand: +
  • coordinates: 807905..810436
  • length: 2532
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (807905..810436) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
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    1981
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    2521
    ATGGGATTTTTATCAAAAATTCTTGATGGCAATAATAAAGAAATTAAACAGTTAGGTAAA
    CTTGCTGATAAAGTAATCGCTTTAGAAGAAAAAACGGCAATTTTAACTGATGAAGAAATT
    CGTAATAAAACGAAACAATTCCAAACAGAATTAGCTGACATTGATAATGTCAAAAAGCAA
    AATGATTATTTAGATAAAATTTTACCAGAAGCATATGCACTTGTTAGAGAAGGCTCTAAA
    CGTGTATTCAATATGACACCATATAAAGTTCAAATTATGGGTGGTATTGCAATTCATAAA
    GGTGATATCGCTGAGATGAGAACAGGTGAAGGTAAAACATTAACAGCGACAATGCCAACA
    TACTTAAATGCATTAGCTGGTAGAGGTGTTCACGTTATTACAGTCAATGAATACTTATCA
    AGTGTTCAAAGTGAAGAAATGGCTGAGTTATATAACTTCTTAGGTTTGACTGTCGGATTA
    AACTTAAACAGTAAGACGACAGAAGAAAAACGTGAAGCATACGCACAAGACATTACTTAC
    AGTACTAATAATGAGCTAGGTTTTGATTACTTACGAGATAACATGGTGAATTATTCTGAA
    GATAGAGTAATGCGTCCATTACATTTTGCAATCATTGATGAGGTTGACTCAATTTTAATC
    GACGAGGCACGTACGCCATTAATTATTTCTGGTGAAGCTGAAAAGTCAACGTCACTTTAT
    ACACAAGCAAATGTTTTTGCGAAAATGTTAAAACAGGACGAAGATTATAAATACGATGAA
    AAAACGAAAGCTGTACATTTAACAGAACAAGGTGCGGATAAAGCTGAACGTATGTTCAAA
    GTTGAAAACTTATATGATGTACAAAATGTTGATGTTATTAGTCATATCAACACAGCTTTA
    CGTGCGCACGTTACATTACAACGTGACGTAGACTATATGGTTGTTGATGGCGAAGTATTA
    ATTGTCGATCAATTTACAGGACGTACAATGCCAGGCCGTCGTTTCTCGGAAGGTTTACAC
    CAAGCTATTGAAGCGAAGGAAGGCGTTCAAATTCAAAATGAATCTAAAACTATGGCGTCT
    ATTACATTCCAAAACTATTTCAGAATGTACAATAAACTTGCGGGTATGACAGGTACAGCT
    AAAACTGAAGAAGAAGAATTTAGAAATATTTATAACATGACAGTAACTCAAATTCCGACA
    AATAAACCTGTGCAACGTAACGATAAGTCTGATTTAATTTACATTAGCCAAAAAGGTAAA
    TTTGATGCAGTAGTAGAAGATGTTGTTGAAAAACACAAGGCAGGGCAACCAGTGCTATTA
    GGTACTGTTGCAGTTGAGACTTCTGAATATATTTCAAATTTACTTAAAAAACGTGGTATC
    CGTCATGATGTGTTAAATGCGAAAAATCATGAACGTGAAGCTGAAATTGTTGCAGGCGCT
    GGACAAAAAGGTGCCGTTACTATTGCCACTAACATGGCTGGTCGTGGTACAGATATCAAA
    TTAGGTGAAGGCGTAGAGGAATTAGGCGGTTTAGCAGTAATAGGTACAGAGCGACATGAA
    TCTCGTCGTATTGATGACCAGTTACGTGGTCGTTCTGGACGTCAAGGTGATAAAGGGGAT
    AGTCGCTTCTATTTATCATTACAAGATGAATTAATGATTCGTTTTGGTTCTGAACGTTTA
    CAGAAAATGATGAGCCGACTAGGTTTAGATGACTCTACACCAATTGAATCAAAAATGGTA
    TCAAGAGCTGTAGAATCAGCACAAAAACGTGTAGAAGGTAATAACTTCGACGCGCGTAAA
    CGTATCTTAGAATACGATGAAGTATTACGTAAACAACGTGAAATTATCTATAACGAAAGA
    AATAGTATTATTGATGAAGAAGACAGCTCTCAAGTTGTAGATGCAATGCTACGTTCAACG
    TTACAACGTAGTATCAATTACTATATTAATACAGCAGATGACGAGCCTGAATATCAACCA
    TTCATCGACTACATTAATGACATCTTCTTACAAGAAGGTGACATTACAGAGGATGATATC
    AAAGGTAAAGATGCTGAAGATATTTTCGAAGTCGTTTGGGCTAAGATTGAAGCAGCATAT
    CAAAGTCAAAAAGATATCTTAGAAGAACAAATGAATGAGTTTGAGCGTATGATTTTACTT
    CGTTCTATTGATAGCCATTGGACTGATCATATCGACACAATGGATCAATTACGTCAAGGT
    ATTCACTTACGTTCTTATGCACAACAAAATCCATTACGTGACTATCAAAATGAAGGTCAT
    GAATTATTTGATATCATGATGCAAAATATTGAAGAAGATACTTGTAAATTCATTTTAAAA
    TCTGTAGTACAAGTTGAAGATAATATTGAACGTGAAAAAACAACAGAGTTTGGTGAAGCG
    AAGCACGTTTCAGCTGAAGATGGTAAAGAAAAAGTGAAACCGAAACCAATCGTTAAAGGC
    GATCAAGTTGGTCGTAACGATGATTGTCCATGTGGTAGTGGTAAAAAATTCAAAAATTGC
    CATGGAAAATAA
    60
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    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2532

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS04080 [old locus tag: NWMN_0722 ]
  • symbol: NWMN_RS04080
  • description: preprotein translocase subunit SecA
  • length: 843
  • theoretical pI: 4.86612
  • theoretical MW: 95959.5
  • GRAVY: -0.62242

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein and peptide secretion and traffickingpreprotein translocase, SecA subunit (TIGR00963; HMM-score: 1129)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingaccessory Sec system translocase SecA2 (TIGR04397; HMM-score: 933.2)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingaccessory Sec system translocase SecA2 (TIGR03714; HMM-score: 852)
    accessory Sec system translocase SecA2 (TIGR04221; HMM-score: 615.2)
    SWIM/SEC-C metal-binding motif protein, PBPRA1643 family (TIGR04102; HMM-score: 25)
  • TheSEED:
  • PFAM:
    P-loop_NTPase (CL0023) SecA_DEAD; SecA DEAD-like domain (PF07517; HMM-score: 397.2)
    no clan definedSecA_SW; SecA Wing and Scaffold domain (PF07516; HMM-score: 193.4)
    SecA_PP_bind; SecA preprotein cross-linking domain (PF01043; HMM-score: 140.4)
    SEC-C; SEC-C motif (PF02810; HMM-score: 35.2)
    P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 20.3)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 15.9)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.118
    • Ymax_pos: 22
    • Cmax: 0.105
    • Cmax_pos: 22
    • Smax: 0.159
    • Smax_pos: 12
    • Smean: 0.125
    • D: 0.121
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446428676 NCBI
  • RefSeq: WP_000506531 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKTKQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGDIAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGLNLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSILIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAERMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRRFSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYNMTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEYISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEELGGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSRLGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSIIDEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKGKDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQGIHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFGEAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS04080 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]