From AureoWiki
Jump to: navigation, search
NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS04730 [old locus tag: NWMN_0839 ]
  • pan locus tag?: SAUPAN003078000
  • symbol: NWMN_RS04730
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS04730 [old locus tag: NWMN_0839 ]
  • symbol: NWMN_RS04730
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 932451..933353
  • length: 903
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (932451..933353) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAATTCTTATCATTCAAGTATAATGACAAAACTTCATATGGCGTTAAAGTAAAACGC
    GAAGATGCTGTATGGGATTTAACACAAGTATTTGCTGACTTTGCAGAAGGAGATTTCCAT
    CCTAAAACATTGTTAGCTGGTTTACAACAAAATCATACTTTAGATTTTCAAGAACAAGTA
    CGTAAAGCAGTTGTAGCAGCAGAAGATAGCGGCAAAGCTGAAGACTATAAAATTTCATTT
    AATGACATTGAATTTTTACCACCAGTAACACCTCCGAATAATGTGATTGCTTTTGGTAGA
    AATTACAAAGATCATGCGAACGAATTAAATCATGAAGTGGAAAAATTATATGTATTTACA
    AAAGCAGCGTCATCTTTAACAGGAGATAATGCAACAATTCCAAATCATAAAGATATTACT
    GATCAATTAGATTATGAAGGTGAATTAGGTATTGTTATTGGTAAGTCTGGTGAAAAGATT
    CCAAAAGCATTAGCTTTAGATTATGTTTACGGCTATACAATTATTAACGATATCACTGAT
    CGCAAAGCACAAAGTGAACAAGATCAAGCATTTTTATCAAAAAGTTTAACTGGCGGTTGC
    CCAATGGGTCCTTATATCGTTACTAAAGACGAACTACCATTACCTGAAAATGTAAATATT
    GTTACAAAAGTTAACAATGAAATTAGACAAGATGGTAACACTGGCGAAATGATTCTTAAA
    ATTGATGAATTAATAGAAGAAATTTCAAAATATGTTGCACTACATCCGGGAGATATTATT
    GCAACTGGTACACCAGCAGGCGTTGGTGCAGGTATGCAACCACCTAAATTTTTACAACCA
    GGTGATGAAGTTAAAGTGACTATTGATAATATTGGAACGCTGACAACTTATATCGCTAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    903

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS04730 [old locus tag: NWMN_0839 ]
  • symbol: NWMN_RS04730
  • description: hypothetical protein
  • length: 300
  • theoretical pI: 4.60314
  • theoretical MW: 33113.1
  • GRAVY: -0.368667

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, C-terminal subunit (TIGR02303; EC 4.1.1.68,5.3.3.10; HMM-score: 174.5)
    4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, N-terminal subunit (TIGR02305; EC 4.1.1.68,5.3.3.10; HMM-score: 96.8)
    MetabolismEnergy metabolismAmino acids and aminesfumarylacetoacetase (TIGR01266; EC 3.7.1.2; HMM-score: 18.5)
  • TheSEED:
  • PFAM:
    FAH (CL0377) FAA_hydrolase; Fumarylacetoacetate (FAA) hydrolase family (PF01557; HMM-score: 206)
    KH (CL0007) KH_2; KH domain (PF07650; HMM-score: 14.4)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.142
    • Ymax_pos: 11
    • Cmax: 0.152
    • Cmax_pos: 33
    • Smax: 0.244
    • Smax_pos: 7
    • Smean: 0.202
    • D: 0.165
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446593406 NCBI
  • RefSeq: WP_000670752 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTLLAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNYKDHANELNHEVEKLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKIPKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENVNIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPKFLQPGDEVKVTIDNIGTLTTYIAK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS04730 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]