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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS08905 [old locus tag: NWMN_1587 ]
  • pan locus tag?: SAUPAN004319000
  • symbol: NWMN_RS08905
  • pan gene symbol?: citC
  • synonym:
  • product: isocitrate dehydrogenase (NADP(+))

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS08905 [old locus tag: NWMN_1587 ]
  • symbol: NWMN_RS08905
  • product: isocitrate dehydrogenase (NADP(+))
  • replicon: chromosome
  • strand: -
  • coordinates: 1758293..1759561
  • length: 1269
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1758293..1759561) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACTGCAGAAAAAATTACTCAAGGAACTGAAGGATTAAACGTACCTAATGAACCAATT
    ATCCCATTTATTATCGGTGATGGAATTGGACCGGATATTTGGAAGGCAGCAAGCCGAGTT
    ATAGATGCTGCTGTTGAGAAAGCCTATAATGGCGAAAAACGCATTGAATGGAAAGAAGTG
    CTAGCTGGCCAAAAAGCATTTGATACAACTGGTGAATGGTTACCTCAAGAAACACTTGAT
    ACAATTAAAGAATATTTAATTGCTGTTAAAGGACCTTTAACAACACCAATTGGTGGTGGT
    ATTAGATCATTAAATGTGGCTTTACGCCAAGAATTAGATTTATTTACTTGCTTAAGACCG
    GTACGTTGGTTTAAAGGAGTACCATCACCTGTTAAACGTCCACAAGATGTTGATATGGTT
    ATTTTCCGTGAAAATACTGAAGACATTTATGCTGGTATTGAATTTAAAGAAGGTACAACA
    GAAGTTAAAAAGGTAATTGACTTCTTACAAAACGAAATGGGTGCGACAAACATTCGATTC
    CCAGAAACTTCAGGTATTGGTATTAAACCAGTTTCTAAAGAAGGAACTGAGCGATTAGTT
    AGAGCAGCTATACAATATGCTATCGATAATAACCGTAAATCAGTTACTTTAGTTCATAAA
    GGTAATATTATGAAATTTACAGAAGGCTCATTTAAGCAGTGGGGTTACGATTTAGCATTA
    TCTGAATTTGGTGATCAAGTATTCACTTGGCAACAATATGACGAAATTGTTGAAAATGAA
    GGCAGAGATGCTGCTAATGCTGCTCAAGAAAAAGCTGAAAAAGAAGGCAAGATTATCATT
    AAAGATTCTATTGCTGACATTTTCTTACAACAAATTTTAACTCGTCCAGCTGAGCATGAT
    GTTGTAGCAACTATGAACTTGAATGGTGACTATATTTCAGATGCTTTAGCTGCACAAGTT
    GGTGGTATTGGTATTGCGCCAGGTGCAAACATTAATTATGAAACAGGTCATGCTATTTTT
    GAAGCAACACATGGTACAGCTCCAAAATATGCAGGTTTAAATAAAGTGAATCCATCTTCA
    GTAATTTTAAGTTCTGTATTAATGTTAGAACATTTAGGATGGCAAGAAGCGGCAGATAAG
    ATTACAGATTCAATTGAAGATACAATTGCTTCAAAAGTTGTTACTTATGACTTTGCCCGT
    TTAATGGATGGTGCTGAAGAAGTTTCTACATCAGCATTTGCAGATGAATTGATTAAAAAT
    TTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1269

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS08905 [old locus tag: NWMN_1587 ]
  • symbol: NWMN_RS08905
  • description: isocitrate dehydrogenase (NADP(+))
  • length: 422
  • theoretical pI: 4.56927
  • theoretical MW: 46422.3
  • GRAVY: -0.231043

Function[edit source | edit]

  • reaction:
    EC 1.1.1.42?  ExPASy
    Isocitrate dehydrogenase (NADP+)Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH
    EC 1.1.1.41?  ExPASy
    Isocitrate dehydrogenase (NAD+)Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH
  • TIGRFAM:
    MetabolismEnergy metabolismTCA cycleisocitrate dehydrogenase, NADP-dependent (TIGR00183; EC 1.1.1.42; HMM-score: 700)
    isopropylmalate/isohomocitrate dehydrogenases (TIGR02088; HMM-score: 229.8)
    MetabolismEnergy metabolismTCA cycleisocitrate dehydrogenase (TIGR02924; EC 1.1.1.-; HMM-score: 217.2)
    MetabolismEnergy metabolismTCA cycleisocitrate dehydrogenase, NAD-dependent (TIGR00175; EC 1.1.1.41; HMM-score: 198.8)
    MetabolismEnergy metabolismOthertartrate dehydrogenase (TIGR02089; EC 1.1.1.93; HMM-score: 154.7)
    MetabolismAmino acid biosynthesisPyruvate family3-isopropylmalate dehydrogenase (TIGR00169; EC 1.1.1.85; HMM-score: 124.3)
  • TheSEED:
  • PFAM:
    Iso_DH (CL0270) Iso_dh; Isocitrate/isopropylmalate dehydrogenase (PF00180; HMM-score: 404.4)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.126
    • Ymax_pos: 37
    • Cmax: 0.202
    • Cmax_pos: 37
    • Smax: 0.135
    • Smax_pos: 36
    • Smean: 0.069
    • D: 0.104
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446045310 NCBI
  • RefSeq: WP_000123165 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAAVEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIRSLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTTEVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLVHKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEGKIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYETGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASKVVTYDFARLMDGAEEVSTSAFADELIKNLK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS08905 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]