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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS12410 [old locus tag: NWMN_2149 ]
  • pan locus tag?: SAUPAN005699000
  • symbol: NWMN_RS12410
  • pan gene symbol?: rplB
  • synonym:
  • product: 50S ribosomal protein L2

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS12410 [old locus tag: NWMN_2149 ]
  • symbol: NWMN_RS12410
  • product: 50S ribosomal protein L2
  • replicon: chromosome
  • strand: -
  • coordinates: 2373078..2373911
  • length: 834
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (2373078..2373911) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTATTAAAAAGTATAAGCCAATAACAAATGGTCGTCGTAATATGACTTCGTTAGAT
    TTCGCAGAAATCACGAAAACTACACCTGAAAAGTCATTATTAAAACCGCTACCGAAAAAA
    GCGGGACGTAACAACCAAGGTAAATTGACTGTAAGACACCATGGTGGTGGACACAAACGT
    CAATACCGTGTTATCGATTTCAAACGTAACAAAGATGGTATCAATGCAAAAGTTGATTCT
    ATTCAATATGATCCAAACCGCTCAGCAAACATCGCTTTAGTTGTATATGCAGACGGTGAA
    AAACGATATATCATTGCTCCTAAAGGATTAGAAGTAGGTCAAATCGTTGAAAGTGGTGCT
    GAAGCTGACATCAAAGTTGGTAACGCATTACCATTACAAAACATTCCAGTTGGTACAGTA
    GTACACAACATCGAGCTTAAACCTGGTAAAGGTGGACAAATCGCTCGTTCAGCTGGTGCA
    AGTGCTCAAGTACTTGGTAAAGAAGGTAAATACGTATTAATCAGATTAAGATCTGGTGAA
    GTTCGTATGATCTTATCTACTTGCCGTGCTACAATCGGTCAAGTTGGTAACCTACAACAC
    GAATTAGTTAACGTTGGTAAAGCCGGACGTTCAAGATGGAAAGGTATCCGTCCAACAGTT
    CGTGGTTCTGTAATGAACCCTAACGATCACCCACACGGTGGTGGTGAAGGTCGTGCTCCT
    ATCGGTAGACCATCTCCAATGTCACCATGGGGTAAACCTACGCTTGGTAAGAAAACTCGT
    CGTGGTAAAAAATCATCAGACAAACTTATCGTTCGTGGACGTAAGAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    834

Protein[edit source | edit]

Protein Data Bank: 5LI0
Protein Data Bank: 4WCE
Protein Data Bank: 4WFB
Protein Data Bank: 4WF9
Protein Data Bank: 4WFA
Protein Data Bank: 5HL7

General[edit source | edit]

  • locus tag: NWMN_RS12410 [old locus tag: NWMN_2149 ]
  • symbol: NWMN_RS12410
  • description: 50S ribosomal protein L2
  • length: 277
  • theoretical pI: 11.4477
  • theoretical MW: 30154.8
  • GRAVY: -0.664621

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationribosomal protein uL2 (TIGR01171; HMM-score: 460.3)
  • TheSEED:
  • PFAM:
    KOW (CL0107) Ribosomal_L2_C; Ribosomal Proteins L2, C-terminal domain (PF03947; HMM-score: 192.1)
    OB (CL0021) Ribosomal_L2; Ribosomal Proteins L2, RNA binding domain (PF00181; HMM-score: 122.4)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.174
    • Ymax_pos: 11
    • Cmax: 0.129
    • Cmax_pos: 33
    • Smax: 0.412
    • Smax_pos: 1
    • Smean: 0.285
    • D: 0.217
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 446908216 NCBI
  • RefSeq: WP_000985472 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRNNQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKRYIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGASAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRPTVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKKK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS12410 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]