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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS05110 [old locus tag: NWMN_0912 ]
  • pan locus tag?: SAUPAN003244000
  • symbol: NWMN_RS05110
  • pan gene symbol?: menF
  • synonym:
  • product: isochorismate synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS05110 [old locus tag: NWMN_0912 ]
  • symbol: NWMN_RS05110
  • product: isochorismate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 1009845..1011056
  • length: 1212
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1009845..1011056) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCGTTTGAATGATAATCGAACGTCATATTTTGGCTACAAAGCAATTCAATTATTCAAA
    AATAATTCTAAAAATAAACAATCTATTTTTAAAGACTGGGAAAAATTAAAACATAACATC
    ACATTTATACATCCGCAATCTGAGAAACATCATCTTCGAGTTGTTGGAGGGTTCCAATTT
    TCAAGTCATAAATCAGATGATGAATGGCGAGAGTTTGGACTAAATCATTTTGTATTACCT
    GAAGTTTTAATTTCAACTGATAATAATGGGACATTTTTAACTTATACAGTTAAAAGGGAA
    AGTTTTACTGTTGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATA
    GATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGG
    CGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTAGCA
    CGTAGACGGTTAATAAAGTTCGATAAAGATATCAGTATTCCATATATTCTAAAGCAAGCA
    TATTCTAAAGAAAAAAACAGTTATATATTCTTGTTAGAATCACAAGATTCTATATTCTTT
    TCACAAACACCTGAACAATTAATAAAGGTCAATAATAAAATACTATCGACTAAAGCTGTA
    GCAGGTACAATTAAACGTTCACAAGATGAGGACGAAGATACAAAAAATGTTGAAGCATTT
    TTAAAAGATAATAAAAACTTAATCGAACATCGATTTGTTGTTGACAGTATTTTACATGAT
    ATTAAACCTTATATCACTGAATTACATTATGATAAGACGCCTAAAATTCTAAAAAATGAT
    CATTTATATCACTTGTACACTGAAATAAAGGCGCCACTGAAGGATGATTCGTATATTAGT
    TTAATTGATAATTTACATCCAACACCTGCTTTAGGTGGCTATCCAAAAGAATTTGCAATG
    GATTTTATTGAGCAGAAAGAATTTGGTACGCGAGGATTATATGGTGCACCGGTTGGCTAT
    ATAGATATATACGATGATTGTGAATTTATTGTTGCAATTCGTTCGATGCTTATTAAGAAA
    GCACAAGCAACTTTATTTGCTGGGTGTGGCATTGTTAAAGATTCTGATCCAGATAGTGAA
    TTGGCAGAAACGAACCTTAAGTTCACACCTATGATGAATGCATTAGGAGTCGATATGAAT
    GGGAAATCATAA
    60
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    540
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    840
    900
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    1020
    1080
    1140
    1200
    1212

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS05110 [old locus tag: NWMN_0912 ]
  • symbol: NWMN_RS05110
  • description: isochorismate synthase
  • length: 403
  • theoretical pI: 5.48308
  • theoretical MW: 46618.5
  • GRAVY: -0.481141

Function[edit source | edit]

  • reaction:
    EC 5.4.4.2?  ExPASy
    Isochorismate synthaseChorismate = isochorismate
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneisochorismate synthase (TIGR00543; EC 5.4.4.2; HMM-score: 411.7)
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase component I (TIGR01820; EC 4.1.3.27; HMM-score: 153.5)
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase component I (TIGR00564; EC 4.1.3.27; HMM-score: 139.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersFolic acidaminodeoxychorismate synthase, component I (TIGR00553; EC 2.6.1.85; HMM-score: 119)
    aminodeoxychorismate synthase, component I (TIGR01824; EC 2.6.1.85; HMM-score: 107.4)
    salicylate synthase (TIGR03494; EC 4.1.3.-,5.4.4.2; HMM-score: 90.2)
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase component I (TIGR00565; EC 4.1.3.27; HMM-score: 83.5)
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 60.4)
    aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 59.4)
  • TheSEED:
  • PFAM:
    no clan definedChorismate_bind; chorismate binding enzyme (PF00425; HMM-score: 217.5)
    ATP_synthase (CL0255) vATP-synt_E; ATP synthase (E/31 kDa) subunit (PF01991; HMM-score: 16.5)
    no clan definedSinI; Anti-repressor SinI (PF08671; HMM-score: 9.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.154
    • Ymax_pos: 11
    • Cmax: 0.117
    • Cmax_pos: 29
    • Smax: 0.32
    • Smax_pos: 7
    • Smean: 0.254
    • D: 0.193
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI:
  • RefSeq: WP_001804449 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS05110 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]