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NCBI: 02-MAR-2017

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS06295 [old locus tag: NWMN_1114 ]
  • pan locus tag?: SAUPAN003483000
  • symbol: NWMN_RS06295
  • pan gene symbol?: carB
  • synonym:
  • product: carbamoyl-phosphate synthase large chain

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_RS06295 [old locus tag: NWMN_1114 ]
  • symbol: NWMN_RS06295
  • product: carbamoyl-phosphate synthase large chain
  • replicon: chromosome
  • strand: +
  • coordinates: 1218907..1222080
  • length: 3174
  • essential: unknown

Accession numbers[edit source | edit]

  • Location: NC_009641 (1218907..1222080) NCBI
  • MicrobesOnline:

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

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    ATGCCTAAACGTAATGATATCAAAACAATTTTAGTAATAGGGTCTGGGCCAATTATCATA
    GGTCAAGCAGCTGAATTTGATTATGCTGGAACACAAGCATGTCTAGCTTTAAAAGAAGAG
    GGATATCGAGTTATTCTTGTAAATTCAAATCCAGCGACAATCATGACTGATAAGGAAATT
    GCGGATAAAGTATATATCGAACCGTTAACTCATGATTTTATAGCGCGAATTATACGTAAA
    GAGCAACCTGACGCTTTACTTCCAACTTTAGGTGGTCAAACAGGTTTAAACATGGCGATT
    CAACTACACGAAAGTGGTGTGCTTCAAGATAATAACGTCCAATTATTAGGAACTGAGCTA
    ACATCAATTCAACAAGCAGAAGACCGTGAAATGTTTAGAACATTAATGAATGATTTAAAC
    GTTCCTGTACCAGAGAGTGACATTGTAAATACAGTAGAGCAAGCCTTTAAATTCAAAGAG
    CAAGTGGGATACCCGCTAATTGTTAGACCGGCATTTACGATGGGTGGTACCGGAGGCGGT
    ATTTGTCATAATGATGAAGAATTACATGAAATCGTCTCAAATGGTCTTCATTATAGTCCA
    GCAACGCAATGTTTATTAGAAAAATCTATCGCAGGTTTTAAAGAAATCGAATACGAAGTA
    ATGCGTGATAAAAACGATAATGCCATCGTTGTATGTAACATGGAAAATATTGATCCAGTT
    GGTATTCATACAGGCGATTCAATTGTTGTGGCTCCTAGTCAAACATTATCAGATGTTGAG
    TATCAAATGTTACGTGATGTTTCATTAAAAGTTATTCGAGCTTTAGGTATCGAAGGTGGT
    TGTAATGTTCAATTAGCATTAGATCCCCATTCATTCGATTATTATATTATAGAAGTAAAT
    CCGCGTGTATCACGTTCATCAGCGTTAGCTTCAAAAGCAACAGGATATCCTATTGCAAAA
    TTAGCTGCTAAAATCGCGGTTGGTCTAACATTAGATGAAATGTTAAATCCAATTACAGGA
    ACATCTTATGCAGCGTTTGAACCAACTTTAGACTATGTGATTTCAAAAATACCAAGATTT
    CCTTTTGATAAATTTGAAAAAGGAGAACGAGAGCTTGGCACACAAATGAAAGCAACAGGT
    GAAGTTATGGCCATTGGTCGAACTTACGAAGAATCATTGTTAAAAGCAATTCGATCACTT
    GAGTATGGTGTGCATCACTTAGGATTACCAAATGGTGAAAGCTTCGATCTTGATTATATT
    AAAGAACGTATTTCACACCAAGATGATGAACGATTATTTTTCATCGGCGAAGCAATTAGA
    AGAGGCACAACATTAGAAGAAATTCATAATATGACTCAGATTGATTACTTCTTCTTACAC
    AAGTTCCAAAACATTATTGATATTGAGCATCAATTAAAAGAGCATCAAGGTGATTTAGAA
    TATCTTAAATATGCAAAAGATTATGGATTTAGTGATAAAACAATAGCGCATCGCTTTAAT
    ATGACGGAAGAAGAAGTATATCAATTGCGTATGGAAAATGATATTAAACCTGTTTACAAG
    ATGGTTGATACTTGCGCAGCTGAATTTGAATCTTCAACACCATATTATTATGGTACATAC
    GAAACTGAAAATGAATCCATAGTTACTGACAAAGAAAAAATCTTAGTATTAGGCTCTGGA
    CCAATTCGAATCGGCCAAGGTGTAGAATTTGACTATGCGACAGTTCATGCCGTTTGGGCA
    ATTCAAAAAGCAGGGTACGAAGCGATAATTGTGAATAACAATCCAGAAACAGTTTCAACA
    GACTTCTCAATTTCTGACAAATTATACTTTGAACCTTTAACTGAAGAAGATGTGATGAAT
    ATCATTAATTTAGAAAAACCTAAAGGTGTCGTTGTACAATTTGGAGGACAAACAGCGATT
    AATTTAGCAGACAAATTGGCTAAACATGGTGTTAAAATACTTGGTACTTCACTAGAAAAT
    CTAAATCGTGCTGAAGATAGAAAAGAATTTGAAGCACTATTAAGAAAAATTAACGTGCCA
    CAGCCACAAGGGAAAACAGCTACATCACCTGAGGAAGCATTAGCGAATGCTGCAGAAATC
    GGATATCCGGTTGTAGTAAGACCTTCTTATGTATTAGGTGGTCGCGCAATGGAAATTGTA
    GACAATGACAAAGAGTTAGAAAACTATATGACCCAGGCTGTAAAAGCGAGTCCGGAACAT
    CCGGTACTAGTCGATAGATATTTAACTGGTAAAGAAATTGAAGTTGATGCGATTTGTGAT
    GGAGAAACGGTCATTATTCCAGGAATCATGGAACATATTGAACGTGCTGGTGTGCATAGT
    GGTGACTCAATCGCTGTATATCCACCACAAACTTTGACAGAAGACGAGTTAGCAACACTT
    GAGGACTATACTATAAAATTAGCTAAAGGTTTAAACATCATTGGCTTAATCAACATTCAA
    TTCGTTATAGCTCACGATGGTGTGTATGTTTTAGAAGTAAATCCACGTTCTAGTAGAACG
    GTACCATTCTTAAGTAAAATTACTGATATTCCAATGGCACAATTAGCTATGCGAGCAATC
    ATTGGGGAAAAACTAACAGATATGGGTTATCAAGAAGGGGTTCAACCATATGCTGAGGGT
    GTCTTTGTGAAAGCACCAGTATTTAGTTTTAATAAATTGAAAAATGTTGATATTACTTTA
    GGACCTGAAATGAAGTCAACAGGTGAAGTGATGGGGAAAGATACTACATTAGAAAAGGCG
    TTATTCAAAGGGTTAACAGGTAGTGGCGTTGAAGTTAAAGATCACGGTACAGTATTAATG
    ACCGTCAGTGACAAAGATAAAGAGGAAGTTGTTAAATTGGCACAACGCTTAAATGAAGTT
    GGCTATAAAATTTTAGCAACGTCTGGAACAGCTAATAAATTAGCTGAGTATGACATACCT
    GCAGAAGTAGTAGGCAAAATTGGTGGCGAAAATGATTTATTAACACGTATTCAAAATGGT
    GATGTTCAAATCGTTATAAATACAATGACTAAAGGTAAAGAAGTAGAAAGGGATGGCTTC
    CAAATTAGACGTACTACAGTTGAAAATGGTATTCCATGTTTGACATCTTTAGATACAGCT
    AATGCCTTAACGAATGTAATTGAAAGTATGACATTTACAATGCGTCAAATGTAA
    60
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Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_RS06295 [old locus tag: NWMN_1114 ]
  • symbol: NWMN_RS06295
  • description: carbamoyl-phosphate synthase large chain
  • length: 1057
  • theoretical pI: 4.60041
  • theoretical MW: 117185
  • GRAVY: -0.194229

Function[edit source | edit]

  • reaction:
    EC 6.3.5.5?  ExPASy
    Carbamoyl-phosphate synthase (glutamine-hydrolyzing)2 ATP + L-glutamine + HCO3(-) + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesiscarbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 1581.7)
    MetabolismEnergy metabolismGlycolysis/gluconeogenesispyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 128)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisacetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 99.8)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 96.2)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanD-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 91.7)
    MetabolismCentral intermediary metabolismNitrogen metabolismurea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 81.9)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylamine--glycine ligase (TIGR00877; EC 6.3.4.13; HMM-score: 74.7)
    lysine biosynthesis enzyme LysX (TIGR02144; HMM-score: 68.1)
    Cellular processesCellular processesBiosynthesis of natural productscyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 67.6)
    MetabolismAmino acid biosynthesisOtherpyrrolysine biosynthesis protein PylC (TIGR03909; HMM-score: 66.4)
    alpha-L-glutamate ligase, RimK family (TIGR00768; EC 6.3.2.-; HMM-score: 57.8)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 52.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercoenzyme gamma-F420-2:alpha-L-glutamate ligase (TIGR04443; EC 6.3.2.32; HMM-score: 35.6)
    ATP-grasp enzyme, GAK system (TIGR04356; HMM-score: 27.1)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (TIGR00355; EC 2.1.2.3,3.5.4.10; HMM-score: 21.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminputative heme d1 biosynthesis radical SAM protein NirJ2 (TIGR04055; EC 1.3.99.-; HMM-score: 13.8)
  • TheSEED:
  • PFAM:
    ATP-grasp (CL0179) CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 404.7)
    no clan definedCPSase_L_D3; Carbamoyl-phosphate synthetase large chain, oligomerisation domain (PF02787; HMM-score: 139.6)
    ATP-grasp (CL0179) ATPgrasp_Ter; ATP-grasp in the biosynthetic pathway with Ter operon (PF15632; HMM-score: 96.9)
    no clan definedMGS; MGS-like domain (PF02142; HMM-score: 75.1)
    ATP-grasp (CL0179) ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 73.5)
    Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 67.1)
    ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 64.5)
    GARS_A; Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (PF01071; HMM-score: 56.4)
    ATP-grasp_4; ATP-grasp domain (PF13535; HMM-score: 35.3)
    RimK; RimK-like ATP-grasp domain (PF08443; HMM-score: 30.3)
    ATP-grasp_5; ATP-grasp domain (PF13549; HMM-score: 30.2)
    ATP-grasp_2; ATP-grasp domain (PF08442; HMM-score: 23.7)
    no clan definedPrpR_N; Propionate catabolism activator (PF06506; HMM-score: 12.9)
    TORC_N; Transducer of regulated CREB activity, N terminus (PF12884; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+
  • effectors:
  • protein partners:
    NWMN_RS02965elongation factor Tu  [1] (data from MRSA252)
    NWMN_RS03640LysR family transcriptional regulator  [1] (data from MRSA252)
    NWMN_RS05380pyruvate dehydrogenase E1 component subunit alpha  [1] (data from MRSA252)
    NWMN_RS0657530S ribosomal protein S2  [1] (data from MRSA252)
    NWMN_RS08995universal stress protein UspA  [1] (data from MRSA252)
    NWMN_RS1234030S ribosomal protein S5  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • Ymax: 0.28
    • Ymax_pos: 24
    • Cmax: 0.299
    • Cmax_pos: 24
    • Smax: 0.476
    • Smax_pos: 20
    • Smean: 0.235
    • D: 0.262
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 447049008 NCBI
  • RefSeq: WP_001126264 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRVILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQLHESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNVPVPESDIVNTVEQAFKFKEQVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEYEVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALGIEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEMLNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEESLLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHNMTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQLRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQGVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINLEKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQPQGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEHPVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELATLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLAMRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKDTTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTANKLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENGIPCLTSLDTANALTNVIESMTFTMRQM

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • NWMN_RS06295 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]